Super-Resolution Imaging Reveals Dynamic Reticular Cytoophidia

CTP synthase (CTPS) can form filamentous structures termed cytoophidia in cells in all three domains of life. In order to study the mesoscale structure of cytoophidia, we perform fluorescence recovery after photobleaching (FRAP) and stimulated emission depletion (STED) microscopy in human cells. By...

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Detalles Bibliográficos
Autores principales: Fang, Yi-Fan, Li, Yi-Lan, Li, Xiao-Ming, Liu, Ji-Long
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9569780/
https://www.ncbi.nlm.nih.gov/pubmed/36233000
http://dx.doi.org/10.3390/ijms231911698
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author Fang, Yi-Fan
Li, Yi-Lan
Li, Xiao-Ming
Liu, Ji-Long
author_facet Fang, Yi-Fan
Li, Yi-Lan
Li, Xiao-Ming
Liu, Ji-Long
author_sort Fang, Yi-Fan
collection PubMed
description CTP synthase (CTPS) can form filamentous structures termed cytoophidia in cells in all three domains of life. In order to study the mesoscale structure of cytoophidia, we perform fluorescence recovery after photobleaching (FRAP) and stimulated emission depletion (STED) microscopy in human cells. By using an EGFP dimeric tag as a tool to explore the physical properties of cytoophidia, we find that cytoophidia are dynamic and reticular. The reticular structure of CTPS cytoophidia may provide space for other components, such as IMPDH. In addition, we observe CTPS granules with tentacles.
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spelling pubmed-95697802022-10-17 Super-Resolution Imaging Reveals Dynamic Reticular Cytoophidia Fang, Yi-Fan Li, Yi-Lan Li, Xiao-Ming Liu, Ji-Long Int J Mol Sci Article CTP synthase (CTPS) can form filamentous structures termed cytoophidia in cells in all three domains of life. In order to study the mesoscale structure of cytoophidia, we perform fluorescence recovery after photobleaching (FRAP) and stimulated emission depletion (STED) microscopy in human cells. By using an EGFP dimeric tag as a tool to explore the physical properties of cytoophidia, we find that cytoophidia are dynamic and reticular. The reticular structure of CTPS cytoophidia may provide space for other components, such as IMPDH. In addition, we observe CTPS granules with tentacles. MDPI 2022-10-02 /pmc/articles/PMC9569780/ /pubmed/36233000 http://dx.doi.org/10.3390/ijms231911698 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Fang, Yi-Fan
Li, Yi-Lan
Li, Xiao-Ming
Liu, Ji-Long
Super-Resolution Imaging Reveals Dynamic Reticular Cytoophidia
title Super-Resolution Imaging Reveals Dynamic Reticular Cytoophidia
title_full Super-Resolution Imaging Reveals Dynamic Reticular Cytoophidia
title_fullStr Super-Resolution Imaging Reveals Dynamic Reticular Cytoophidia
title_full_unstemmed Super-Resolution Imaging Reveals Dynamic Reticular Cytoophidia
title_short Super-Resolution Imaging Reveals Dynamic Reticular Cytoophidia
title_sort super-resolution imaging reveals dynamic reticular cytoophidia
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9569780/
https://www.ncbi.nlm.nih.gov/pubmed/36233000
http://dx.doi.org/10.3390/ijms231911698
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AT liyilan superresolutionimagingrevealsdynamicreticularcytoophidia
AT lixiaoming superresolutionimagingrevealsdynamicreticularcytoophidia
AT liujilong superresolutionimagingrevealsdynamicreticularcytoophidia

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