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Directional Submicrofiber Hydrogel Composite Scaffolds Supporting Neuron Differentiation and Enabling Neurite Alignment

Cell cultures aiming at tissue regeneration benefit from scaffolds with physiologically relevant elastic moduli to optimally trigger cell attachment, proliferation and promote differentiation, guidance and tissue maturation. Complex scaffolds designed with guiding cues can mimic the anisotropic natu...

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Autores principales: Mungenast, Lena, Züger, Fabian, Selvi, Jasmin, Faia-Torres, Ana Bela, Rühe, Jürgen, Suter-Dick, Laura, Gullo, Maurizio R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9569964/
https://www.ncbi.nlm.nih.gov/pubmed/36232822
http://dx.doi.org/10.3390/ijms231911525
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author Mungenast, Lena
Züger, Fabian
Selvi, Jasmin
Faia-Torres, Ana Bela
Rühe, Jürgen
Suter-Dick, Laura
Gullo, Maurizio R.
author_facet Mungenast, Lena
Züger, Fabian
Selvi, Jasmin
Faia-Torres, Ana Bela
Rühe, Jürgen
Suter-Dick, Laura
Gullo, Maurizio R.
author_sort Mungenast, Lena
collection PubMed
description Cell cultures aiming at tissue regeneration benefit from scaffolds with physiologically relevant elastic moduli to optimally trigger cell attachment, proliferation and promote differentiation, guidance and tissue maturation. Complex scaffolds designed with guiding cues can mimic the anisotropic nature of neural tissues, such as spinal cord or brain, and recall the ability of human neural progenitor cells to differentiate and align. This work introduces a cost-efficient gelatin-based submicron patterned hydrogel–fiber composite with tuned stiffness, able to support cell attachment, differentiation and alignment of neurons derived from human progenitor cells. The enzymatically crosslinked gelatin-based hydrogels were generated with stiffnesses from 8 to 80 kPa, onto which poly(ε-caprolactone) (PCL) alignment cues were electrospun such that the fibers had a preferential alignment. The fiber–hydrogel composites with a modulus of about 20 kPa showed the strongest cell attachment and highest cell proliferation, rendering them an ideal differentiation support. Differentiated neurons aligned and bundled their neurites along the aligned PCL filaments, which is unique to this cell type on a fiber–hydrogel composite. This novel scaffold relies on robust and inexpensive technology and is suitable for neural tissue engineering where directional neuron alignment is required, such as in the spinal cord.
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spelling pubmed-95699642022-10-17 Directional Submicrofiber Hydrogel Composite Scaffolds Supporting Neuron Differentiation and Enabling Neurite Alignment Mungenast, Lena Züger, Fabian Selvi, Jasmin Faia-Torres, Ana Bela Rühe, Jürgen Suter-Dick, Laura Gullo, Maurizio R. Int J Mol Sci Article Cell cultures aiming at tissue regeneration benefit from scaffolds with physiologically relevant elastic moduli to optimally trigger cell attachment, proliferation and promote differentiation, guidance and tissue maturation. Complex scaffolds designed with guiding cues can mimic the anisotropic nature of neural tissues, such as spinal cord or brain, and recall the ability of human neural progenitor cells to differentiate and align. This work introduces a cost-efficient gelatin-based submicron patterned hydrogel–fiber composite with tuned stiffness, able to support cell attachment, differentiation and alignment of neurons derived from human progenitor cells. The enzymatically crosslinked gelatin-based hydrogels were generated with stiffnesses from 8 to 80 kPa, onto which poly(ε-caprolactone) (PCL) alignment cues were electrospun such that the fibers had a preferential alignment. The fiber–hydrogel composites with a modulus of about 20 kPa showed the strongest cell attachment and highest cell proliferation, rendering them an ideal differentiation support. Differentiated neurons aligned and bundled their neurites along the aligned PCL filaments, which is unique to this cell type on a fiber–hydrogel composite. This novel scaffold relies on robust and inexpensive technology and is suitable for neural tissue engineering where directional neuron alignment is required, such as in the spinal cord. MDPI 2022-09-29 /pmc/articles/PMC9569964/ /pubmed/36232822 http://dx.doi.org/10.3390/ijms231911525 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mungenast, Lena
Züger, Fabian
Selvi, Jasmin
Faia-Torres, Ana Bela
Rühe, Jürgen
Suter-Dick, Laura
Gullo, Maurizio R.
Directional Submicrofiber Hydrogel Composite Scaffolds Supporting Neuron Differentiation and Enabling Neurite Alignment
title Directional Submicrofiber Hydrogel Composite Scaffolds Supporting Neuron Differentiation and Enabling Neurite Alignment
title_full Directional Submicrofiber Hydrogel Composite Scaffolds Supporting Neuron Differentiation and Enabling Neurite Alignment
title_fullStr Directional Submicrofiber Hydrogel Composite Scaffolds Supporting Neuron Differentiation and Enabling Neurite Alignment
title_full_unstemmed Directional Submicrofiber Hydrogel Composite Scaffolds Supporting Neuron Differentiation and Enabling Neurite Alignment
title_short Directional Submicrofiber Hydrogel Composite Scaffolds Supporting Neuron Differentiation and Enabling Neurite Alignment
title_sort directional submicrofiber hydrogel composite scaffolds supporting neuron differentiation and enabling neurite alignment
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9569964/
https://www.ncbi.nlm.nih.gov/pubmed/36232822
http://dx.doi.org/10.3390/ijms231911525
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