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Studying Synaptic Connectivity and Strength with Optogenetics and Patch-Clamp Electrophysiology

Over the last two decades the combination of brain slice patch clamp electrophysiology with optogenetic stimulation has proven to be a powerful approach to analyze the architecture of neural circuits and (experience-dependent) synaptic plasticity in such networks. Using this combination of methods,...

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Autores principales: Linders, Louisa E., Supiot, Laura. F., Du, Wenjie, D’Angelo, Roberto, Adan, Roger A. H., Riga, Danai, Meye, Frank J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9570045/
https://www.ncbi.nlm.nih.gov/pubmed/36232917
http://dx.doi.org/10.3390/ijms231911612
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author Linders, Louisa E.
Supiot, Laura. F.
Du, Wenjie
D’Angelo, Roberto
Adan, Roger A. H.
Riga, Danai
Meye, Frank J.
author_facet Linders, Louisa E.
Supiot, Laura. F.
Du, Wenjie
D’Angelo, Roberto
Adan, Roger A. H.
Riga, Danai
Meye, Frank J.
author_sort Linders, Louisa E.
collection PubMed
description Over the last two decades the combination of brain slice patch clamp electrophysiology with optogenetic stimulation has proven to be a powerful approach to analyze the architecture of neural circuits and (experience-dependent) synaptic plasticity in such networks. Using this combination of methods, originally termed channelrhodopsin-assisted circuit mapping (CRACM), a multitude of measures of synaptic functioning can be taken. The current review discusses their rationale, current applications in the field, and their associated caveats. Specifically, the review addresses: (1) How to assess the presence of synaptic connections, both in terms of ionotropic versus metabotropic receptor signaling, and in terms of mono- versus polysynaptic connectivity. (2) How to acquire and interpret measures for synaptic strength and function, like AMPAR/NMDAR, AMPAR rectification, paired-pulse ratio (PPR), coefficient of variance and input-specific quantal sizes. We also address how synaptic modulation by G protein-coupled receptors can be studied with pharmacological approaches and advanced technology. (3) Finally, we elaborate on advances on the use of dual color optogenetics in concurrent investigation of multiple synaptic pathways. Overall, with this review we seek to provide practical insights into the methods used to study neural circuits and synapses, by combining optogenetics and patch-clamp electrophysiology.
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spelling pubmed-95700452022-10-17 Studying Synaptic Connectivity and Strength with Optogenetics and Patch-Clamp Electrophysiology Linders, Louisa E. Supiot, Laura. F. Du, Wenjie D’Angelo, Roberto Adan, Roger A. H. Riga, Danai Meye, Frank J. Int J Mol Sci Review Over the last two decades the combination of brain slice patch clamp electrophysiology with optogenetic stimulation has proven to be a powerful approach to analyze the architecture of neural circuits and (experience-dependent) synaptic plasticity in such networks. Using this combination of methods, originally termed channelrhodopsin-assisted circuit mapping (CRACM), a multitude of measures of synaptic functioning can be taken. The current review discusses their rationale, current applications in the field, and their associated caveats. Specifically, the review addresses: (1) How to assess the presence of synaptic connections, both in terms of ionotropic versus metabotropic receptor signaling, and in terms of mono- versus polysynaptic connectivity. (2) How to acquire and interpret measures for synaptic strength and function, like AMPAR/NMDAR, AMPAR rectification, paired-pulse ratio (PPR), coefficient of variance and input-specific quantal sizes. We also address how synaptic modulation by G protein-coupled receptors can be studied with pharmacological approaches and advanced technology. (3) Finally, we elaborate on advances on the use of dual color optogenetics in concurrent investigation of multiple synaptic pathways. Overall, with this review we seek to provide practical insights into the methods used to study neural circuits and synapses, by combining optogenetics and patch-clamp electrophysiology. MDPI 2022-10-01 /pmc/articles/PMC9570045/ /pubmed/36232917 http://dx.doi.org/10.3390/ijms231911612 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Linders, Louisa E.
Supiot, Laura. F.
Du, Wenjie
D’Angelo, Roberto
Adan, Roger A. H.
Riga, Danai
Meye, Frank J.
Studying Synaptic Connectivity and Strength with Optogenetics and Patch-Clamp Electrophysiology
title Studying Synaptic Connectivity and Strength with Optogenetics and Patch-Clamp Electrophysiology
title_full Studying Synaptic Connectivity and Strength with Optogenetics and Patch-Clamp Electrophysiology
title_fullStr Studying Synaptic Connectivity and Strength with Optogenetics and Patch-Clamp Electrophysiology
title_full_unstemmed Studying Synaptic Connectivity and Strength with Optogenetics and Patch-Clamp Electrophysiology
title_short Studying Synaptic Connectivity and Strength with Optogenetics and Patch-Clamp Electrophysiology
title_sort studying synaptic connectivity and strength with optogenetics and patch-clamp electrophysiology
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9570045/
https://www.ncbi.nlm.nih.gov/pubmed/36232917
http://dx.doi.org/10.3390/ijms231911612
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