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Characterization of the ABC Transporter G Subfamily in Pomegranate and Function Analysis of PgrABCG14

ATP-binding cassette subfamily G (ABCG) proteins play important roles in plant growth and development by transporting metabolites across cell membranes. To date, the genetic characteristics and potential functions of pomegranate ABCG proteins (PgrABCGs) have remained largely unknown. In this study,...

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Autores principales: Yu, Qing, Li, Jiyu, Qin, Gaihua, Liu, Chunyan, Cao, Zhen, Jia, Botao, Xu, Yiliu, Li, Guixiang, Yang, Yuan, Su, Ying, Zhang, Huping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9570063/
https://www.ncbi.nlm.nih.gov/pubmed/36232964
http://dx.doi.org/10.3390/ijms231911661
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author Yu, Qing
Li, Jiyu
Qin, Gaihua
Liu, Chunyan
Cao, Zhen
Jia, Botao
Xu, Yiliu
Li, Guixiang
Yang, Yuan
Su, Ying
Zhang, Huping
author_facet Yu, Qing
Li, Jiyu
Qin, Gaihua
Liu, Chunyan
Cao, Zhen
Jia, Botao
Xu, Yiliu
Li, Guixiang
Yang, Yuan
Su, Ying
Zhang, Huping
author_sort Yu, Qing
collection PubMed
description ATP-binding cassette subfamily G (ABCG) proteins play important roles in plant growth and development by transporting metabolites across cell membranes. To date, the genetic characteristics and potential functions of pomegranate ABCG proteins (PgrABCGs) have remained largely unknown. In this study, we found that 47 PgrABCGs were divided into five groups according to a phylogenetic analysis; groups I, II, III, and IV members are half-size proteins, and group V members are full-size proteins. PgrABCG14, PgrABCG21, and PgrABCG47 were highly expressed in the inner seed coat but had very low expression levels in the outer seed coat, and the expression levels of these three PgrABCG genes in the inner seed coats of hard-seeded pomegranate ‘Dabenzi’ were higher than those of soft-seeded pomegranate ‘Tunisia’. In addition, the expression of these three PgrABCG genes was highly correlated with the expression of genes involved in lignin biosynthesis and hormone signaling pathways. The evolution of PgrABCG14 presents a highly similar trend to the origin and evolution of lignin biosynthesis during land plant evolution. Ectopic expression of PgrABCG14 in Arabidopsis promoted plant growth and lignin accumulation compared to wild type plants; meanwhile, the expression levels of lignin biosynthesis-related genes (CAD5, C4H, and Prx71) and cytokinin response marker genes (ARR5 and ARR15) were significantly upregulated in transgenic plants, which suggests the potential role of PgrABCG14 in promoting plant growth and lignin accumulation. Taken together, these findings not only provide insight into the characteristics and evolution of PgrABCGs, but also shed a light on the potential functions of PgrABCGs in seed hardness development.
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spelling pubmed-95700632022-10-17 Characterization of the ABC Transporter G Subfamily in Pomegranate and Function Analysis of PgrABCG14 Yu, Qing Li, Jiyu Qin, Gaihua Liu, Chunyan Cao, Zhen Jia, Botao Xu, Yiliu Li, Guixiang Yang, Yuan Su, Ying Zhang, Huping Int J Mol Sci Article ATP-binding cassette subfamily G (ABCG) proteins play important roles in plant growth and development by transporting metabolites across cell membranes. To date, the genetic characteristics and potential functions of pomegranate ABCG proteins (PgrABCGs) have remained largely unknown. In this study, we found that 47 PgrABCGs were divided into five groups according to a phylogenetic analysis; groups I, II, III, and IV members are half-size proteins, and group V members are full-size proteins. PgrABCG14, PgrABCG21, and PgrABCG47 were highly expressed in the inner seed coat but had very low expression levels in the outer seed coat, and the expression levels of these three PgrABCG genes in the inner seed coats of hard-seeded pomegranate ‘Dabenzi’ were higher than those of soft-seeded pomegranate ‘Tunisia’. In addition, the expression of these three PgrABCG genes was highly correlated with the expression of genes involved in lignin biosynthesis and hormone signaling pathways. The evolution of PgrABCG14 presents a highly similar trend to the origin and evolution of lignin biosynthesis during land plant evolution. Ectopic expression of PgrABCG14 in Arabidopsis promoted plant growth and lignin accumulation compared to wild type plants; meanwhile, the expression levels of lignin biosynthesis-related genes (CAD5, C4H, and Prx71) and cytokinin response marker genes (ARR5 and ARR15) were significantly upregulated in transgenic plants, which suggests the potential role of PgrABCG14 in promoting plant growth and lignin accumulation. Taken together, these findings not only provide insight into the characteristics and evolution of PgrABCGs, but also shed a light on the potential functions of PgrABCGs in seed hardness development. MDPI 2022-10-01 /pmc/articles/PMC9570063/ /pubmed/36232964 http://dx.doi.org/10.3390/ijms231911661 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yu, Qing
Li, Jiyu
Qin, Gaihua
Liu, Chunyan
Cao, Zhen
Jia, Botao
Xu, Yiliu
Li, Guixiang
Yang, Yuan
Su, Ying
Zhang, Huping
Characterization of the ABC Transporter G Subfamily in Pomegranate and Function Analysis of PgrABCG14
title Characterization of the ABC Transporter G Subfamily in Pomegranate and Function Analysis of PgrABCG14
title_full Characterization of the ABC Transporter G Subfamily in Pomegranate and Function Analysis of PgrABCG14
title_fullStr Characterization of the ABC Transporter G Subfamily in Pomegranate and Function Analysis of PgrABCG14
title_full_unstemmed Characterization of the ABC Transporter G Subfamily in Pomegranate and Function Analysis of PgrABCG14
title_short Characterization of the ABC Transporter G Subfamily in Pomegranate and Function Analysis of PgrABCG14
title_sort characterization of the abc transporter g subfamily in pomegranate and function analysis of pgrabcg14
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9570063/
https://www.ncbi.nlm.nih.gov/pubmed/36232964
http://dx.doi.org/10.3390/ijms231911661
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