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Macropore Regulation of Hydroxyapatite Osteoinduction via Microfluidic Pathway

Macroporous characteristics have been shown to play a key role in the osteoinductivity of hydroxyapatite ceramics, but the physics underlying the new bone formation and distribution in such scaffolds still remain elusive. The work here has emphasized the osteoinductive capacity of porous hydroxyapat...

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Autores principales: Shi, Feng, Fang, Xin, Zhou, Teng, Huang, Xu, Duan, Ke, Wang, Jianxin, Qu, Shuxin, Zhi, Wei, Weng, Jie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9570064/
https://www.ncbi.nlm.nih.gov/pubmed/36232757
http://dx.doi.org/10.3390/ijms231911459
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author Shi, Feng
Fang, Xin
Zhou, Teng
Huang, Xu
Duan, Ke
Wang, Jianxin
Qu, Shuxin
Zhi, Wei
Weng, Jie
author_facet Shi, Feng
Fang, Xin
Zhou, Teng
Huang, Xu
Duan, Ke
Wang, Jianxin
Qu, Shuxin
Zhi, Wei
Weng, Jie
author_sort Shi, Feng
collection PubMed
description Macroporous characteristics have been shown to play a key role in the osteoinductivity of hydroxyapatite ceramics, but the physics underlying the new bone formation and distribution in such scaffolds still remain elusive. The work here has emphasized the osteoinductive capacity of porous hydroxyapatite scaffolds containing different macroporous sizes (200–400 μm, 1200–1500 μm) and geometries (star shape, spherical shape). The assumption is that both the size and shape of a macropore structure may affect the microfluidic pathways in the scaffolds, which results in the different bone formations and distribution. Herein, a mathematical model and an animal experiment were proposed to support this hypothesis. The results showed that the porous scaffolds with the spherical macropores and large pore sizes (1200–1500 μm) had higher new bone production and more uniform new bone distribution than others. A finite element analysis suggested that the macropore shape affected the distribution of the medium–high velocity flow field, while the macropore size effected microfluid speed and the value of the shear stress in the scaffolds. Additionally, the result of scaffolds implanted into the dorsal muscle having a higher new bone mass than the abdominal cavity suggested that the mechanical load of the host tissue could play a key role in the microfluidic pathway mechanism. All these findings suggested that the osteoinduction of these scaffolds depends on both the microfluid velocity and shear stress generated by the macropore size and shape. This study, therefore, provides new insights into the inherent osteoinductive mechanisms of bioceramics, and may offer clues toward a rational design of bioceramic scaffolds with improved osteoinductivity.
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spelling pubmed-95700642022-10-17 Macropore Regulation of Hydroxyapatite Osteoinduction via Microfluidic Pathway Shi, Feng Fang, Xin Zhou, Teng Huang, Xu Duan, Ke Wang, Jianxin Qu, Shuxin Zhi, Wei Weng, Jie Int J Mol Sci Article Macroporous characteristics have been shown to play a key role in the osteoinductivity of hydroxyapatite ceramics, but the physics underlying the new bone formation and distribution in such scaffolds still remain elusive. The work here has emphasized the osteoinductive capacity of porous hydroxyapatite scaffolds containing different macroporous sizes (200–400 μm, 1200–1500 μm) and geometries (star shape, spherical shape). The assumption is that both the size and shape of a macropore structure may affect the microfluidic pathways in the scaffolds, which results in the different bone formations and distribution. Herein, a mathematical model and an animal experiment were proposed to support this hypothesis. The results showed that the porous scaffolds with the spherical macropores and large pore sizes (1200–1500 μm) had higher new bone production and more uniform new bone distribution than others. A finite element analysis suggested that the macropore shape affected the distribution of the medium–high velocity flow field, while the macropore size effected microfluid speed and the value of the shear stress in the scaffolds. Additionally, the result of scaffolds implanted into the dorsal muscle having a higher new bone mass than the abdominal cavity suggested that the mechanical load of the host tissue could play a key role in the microfluidic pathway mechanism. All these findings suggested that the osteoinduction of these scaffolds depends on both the microfluid velocity and shear stress generated by the macropore size and shape. This study, therefore, provides new insights into the inherent osteoinductive mechanisms of bioceramics, and may offer clues toward a rational design of bioceramic scaffolds with improved osteoinductivity. MDPI 2022-09-28 /pmc/articles/PMC9570064/ /pubmed/36232757 http://dx.doi.org/10.3390/ijms231911459 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Shi, Feng
Fang, Xin
Zhou, Teng
Huang, Xu
Duan, Ke
Wang, Jianxin
Qu, Shuxin
Zhi, Wei
Weng, Jie
Macropore Regulation of Hydroxyapatite Osteoinduction via Microfluidic Pathway
title Macropore Regulation of Hydroxyapatite Osteoinduction via Microfluidic Pathway
title_full Macropore Regulation of Hydroxyapatite Osteoinduction via Microfluidic Pathway
title_fullStr Macropore Regulation of Hydroxyapatite Osteoinduction via Microfluidic Pathway
title_full_unstemmed Macropore Regulation of Hydroxyapatite Osteoinduction via Microfluidic Pathway
title_short Macropore Regulation of Hydroxyapatite Osteoinduction via Microfluidic Pathway
title_sort macropore regulation of hydroxyapatite osteoinduction via microfluidic pathway
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9570064/
https://www.ncbi.nlm.nih.gov/pubmed/36232757
http://dx.doi.org/10.3390/ijms231911459
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