Cargando…

Experimental Research Models to Assess the Cross-Reactivity between Can f 5 and Human PSA—Two Different Perspectives

The similarity in antigenic structures, including the degree of correspondence between the amino acid sequence and the spatial structure, is not always reflected in the actual cross-reactivity of allergens. Inhibition tests appear to be an invaluable tool for assessing potential cross-reactivity bet...

Descripción completa

Detalles Bibliográficos
Autores principales: Lis, Kinga, Ukleja-Sokołowska, Natalia, Adamczak, Rafał, Bartuzi, Zbigniew
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9570149/
https://www.ncbi.nlm.nih.gov/pubmed/36232522
http://dx.doi.org/10.3390/ijms231911223
_version_ 1784810034717261824
author Lis, Kinga
Ukleja-Sokołowska, Natalia
Adamczak, Rafał
Bartuzi, Zbigniew
author_facet Lis, Kinga
Ukleja-Sokołowska, Natalia
Adamczak, Rafał
Bartuzi, Zbigniew
author_sort Lis, Kinga
collection PubMed
description The similarity in antigenic structures, including the degree of correspondence between the amino acid sequence and the spatial structure, is not always reflected in the actual cross-reactivity of allergens. Inhibition tests appear to be an invaluable tool for assessing potential cross-reactivity between allergens under natural conditions. In this publication, we present two experimental models of inhibition tests: solid phase (SP-IT) and liquid phase (LP-IT). As an exemplary research model, we used the cross-reactivity between human PSA and anti-Can f 5 IgE antibodies. We performed an SP-IT model using a microplate coated with human PSA. The LP-IT model was based on mixing anti-Can f 5 IgE positive serum with a material containing human PSA. Anti-Can f 5 IgE and PSA concentrations before and after inhibition were measured to evaluate inhibition effectiveness. The results of the performed experiments showed that both proposed models of inhibition tests are an effective tools for studying cross-reactive relationships between antigens. In the SP-IT, the concentration of anti-Can f 5 decreased by 21.6% and, in the LP-IT, it decreased by 34.51%. In turn, the PSA concentration in the SP-IT decreased by 11.25% and, in the LP-IT, it decreased by 15.49%. In conclusion, both the SP-IT and the LP-IT seem to be effective tools for assessing the actual cross-reactivity between different allergens.
format Online
Article
Text
id pubmed-9570149
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-95701492022-10-17 Experimental Research Models to Assess the Cross-Reactivity between Can f 5 and Human PSA—Two Different Perspectives Lis, Kinga Ukleja-Sokołowska, Natalia Adamczak, Rafał Bartuzi, Zbigniew Int J Mol Sci Article The similarity in antigenic structures, including the degree of correspondence between the amino acid sequence and the spatial structure, is not always reflected in the actual cross-reactivity of allergens. Inhibition tests appear to be an invaluable tool for assessing potential cross-reactivity between allergens under natural conditions. In this publication, we present two experimental models of inhibition tests: solid phase (SP-IT) and liquid phase (LP-IT). As an exemplary research model, we used the cross-reactivity between human PSA and anti-Can f 5 IgE antibodies. We performed an SP-IT model using a microplate coated with human PSA. The LP-IT model was based on mixing anti-Can f 5 IgE positive serum with a material containing human PSA. Anti-Can f 5 IgE and PSA concentrations before and after inhibition were measured to evaluate inhibition effectiveness. The results of the performed experiments showed that both proposed models of inhibition tests are an effective tools for studying cross-reactive relationships between antigens. In the SP-IT, the concentration of anti-Can f 5 decreased by 21.6% and, in the LP-IT, it decreased by 34.51%. In turn, the PSA concentration in the SP-IT decreased by 11.25% and, in the LP-IT, it decreased by 15.49%. In conclusion, both the SP-IT and the LP-IT seem to be effective tools for assessing the actual cross-reactivity between different allergens. MDPI 2022-09-23 /pmc/articles/PMC9570149/ /pubmed/36232522 http://dx.doi.org/10.3390/ijms231911223 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lis, Kinga
Ukleja-Sokołowska, Natalia
Adamczak, Rafał
Bartuzi, Zbigniew
Experimental Research Models to Assess the Cross-Reactivity between Can f 5 and Human PSA—Two Different Perspectives
title Experimental Research Models to Assess the Cross-Reactivity between Can f 5 and Human PSA—Two Different Perspectives
title_full Experimental Research Models to Assess the Cross-Reactivity between Can f 5 and Human PSA—Two Different Perspectives
title_fullStr Experimental Research Models to Assess the Cross-Reactivity between Can f 5 and Human PSA—Two Different Perspectives
title_full_unstemmed Experimental Research Models to Assess the Cross-Reactivity between Can f 5 and Human PSA—Two Different Perspectives
title_short Experimental Research Models to Assess the Cross-Reactivity between Can f 5 and Human PSA—Two Different Perspectives
title_sort experimental research models to assess the cross-reactivity between can f 5 and human psa—two different perspectives
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9570149/
https://www.ncbi.nlm.nih.gov/pubmed/36232522
http://dx.doi.org/10.3390/ijms231911223
work_keys_str_mv AT liskinga experimentalresearchmodelstoassessthecrossreactivitybetweencanf5andhumanpsatwodifferentperspectives
AT uklejasokołowskanatalia experimentalresearchmodelstoassessthecrossreactivitybetweencanf5andhumanpsatwodifferentperspectives
AT adamczakrafał experimentalresearchmodelstoassessthecrossreactivitybetweencanf5andhumanpsatwodifferentperspectives
AT bartuzizbigniew experimentalresearchmodelstoassessthecrossreactivitybetweencanf5andhumanpsatwodifferentperspectives