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Temperature-Biased miRNA Expression Patterns during European Sea Bass (Dicentrarchus labrax) Development
Environmental effects and, particularly, temperature changes have been demonstrated to influence the activity, function, and well-being of teleosts. Temperature may change seasonally in the wild, and in captivity under aquaculture operations. Moreover, climate change is expected to shift temperature...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9570215/ https://www.ncbi.nlm.nih.gov/pubmed/36232462 http://dx.doi.org/10.3390/ijms231911164 |
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author | Papadaki, Maria Kaitetzidou, Elisavet Papadakis, Ioannis E. Sfakianakis, Dimitris G. Papandroulakis, Nikos Mylonas, Constantinos C. Sarropoulou, Elena |
author_facet | Papadaki, Maria Kaitetzidou, Elisavet Papadakis, Ioannis E. Sfakianakis, Dimitris G. Papandroulakis, Nikos Mylonas, Constantinos C. Sarropoulou, Elena |
author_sort | Papadaki, Maria |
collection | PubMed |
description | Environmental effects and, particularly, temperature changes have been demonstrated to influence the activity, function, and well-being of teleosts. Temperature may change seasonally in the wild, and in captivity under aquaculture operations. Moreover, climate change is expected to shift temperature profiles worldwide. MicroRNAs (miRNA) are important temperature-sensitive gene-expression regulators acting at the post-transcriptional level. They are known to be key regulators in development, reproduction, and immune responses. Therefore, early larval development of the European sea bass (Dicentrarchus labrax), one of the most extensively cultured species in Mediterranean aquaculture, was investigated at early rearing temperatures, i.e., 15, 17.5, and 20 °C, in regard to the impact of temperatures on miRNAs through sncRNA high-throughput sequencing but also at the phenotypic level in terms of growth, sex, vision, and skeletal deformities. Expression profiling revealed stage- and temperature-specific miRNA expression targeting genes with roles in reproduction and immune response mainly at the flexion and all-fins stages. Similar stage- and temperature-specific results were also observed concerning the number of rod cells and lower jaw elongation. The present work presents for the first time highly promising results on the influence of early rearing temperature at the post-transcriptional level during European sea bass development, with a putative impact on reproduction and immune response, as well as regarding teleost vision and larval development. |
format | Online Article Text |
id | pubmed-9570215 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-95702152022-10-17 Temperature-Biased miRNA Expression Patterns during European Sea Bass (Dicentrarchus labrax) Development Papadaki, Maria Kaitetzidou, Elisavet Papadakis, Ioannis E. Sfakianakis, Dimitris G. Papandroulakis, Nikos Mylonas, Constantinos C. Sarropoulou, Elena Int J Mol Sci Article Environmental effects and, particularly, temperature changes have been demonstrated to influence the activity, function, and well-being of teleosts. Temperature may change seasonally in the wild, and in captivity under aquaculture operations. Moreover, climate change is expected to shift temperature profiles worldwide. MicroRNAs (miRNA) are important temperature-sensitive gene-expression regulators acting at the post-transcriptional level. They are known to be key regulators in development, reproduction, and immune responses. Therefore, early larval development of the European sea bass (Dicentrarchus labrax), one of the most extensively cultured species in Mediterranean aquaculture, was investigated at early rearing temperatures, i.e., 15, 17.5, and 20 °C, in regard to the impact of temperatures on miRNAs through sncRNA high-throughput sequencing but also at the phenotypic level in terms of growth, sex, vision, and skeletal deformities. Expression profiling revealed stage- and temperature-specific miRNA expression targeting genes with roles in reproduction and immune response mainly at the flexion and all-fins stages. Similar stage- and temperature-specific results were also observed concerning the number of rod cells and lower jaw elongation. The present work presents for the first time highly promising results on the influence of early rearing temperature at the post-transcriptional level during European sea bass development, with a putative impact on reproduction and immune response, as well as regarding teleost vision and larval development. MDPI 2022-09-22 /pmc/articles/PMC9570215/ /pubmed/36232462 http://dx.doi.org/10.3390/ijms231911164 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Papadaki, Maria Kaitetzidou, Elisavet Papadakis, Ioannis E. Sfakianakis, Dimitris G. Papandroulakis, Nikos Mylonas, Constantinos C. Sarropoulou, Elena Temperature-Biased miRNA Expression Patterns during European Sea Bass (Dicentrarchus labrax) Development |
title | Temperature-Biased miRNA Expression Patterns during European Sea Bass (Dicentrarchus labrax) Development |
title_full | Temperature-Biased miRNA Expression Patterns during European Sea Bass (Dicentrarchus labrax) Development |
title_fullStr | Temperature-Biased miRNA Expression Patterns during European Sea Bass (Dicentrarchus labrax) Development |
title_full_unstemmed | Temperature-Biased miRNA Expression Patterns during European Sea Bass (Dicentrarchus labrax) Development |
title_short | Temperature-Biased miRNA Expression Patterns during European Sea Bass (Dicentrarchus labrax) Development |
title_sort | temperature-biased mirna expression patterns during european sea bass (dicentrarchus labrax) development |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9570215/ https://www.ncbi.nlm.nih.gov/pubmed/36232462 http://dx.doi.org/10.3390/ijms231911164 |
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