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Genome-Wide Identification and Characterization of Banana Ca(2+)-ATPase Genes and Expression Analysis under Different Concentrations of Ca(2+) Treatments

Ca(2+)-ATPases have been confirmed to play very important roles in plant growth and development and in stress responses. However, studies on banana (Musa acuminata) Ca(2+)-ATPases are very limited. In this study, we identified 18 Ca(2+)-ATPase genes from banana, including 6 P-IIA or ER (Endoplasmic...

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Autores principales: Ma, Ronghui, Tian, Na, Wang, Jiashui, Fan, Minlei, Wang, Bin, Qu, Pengyan, Xu, Shiyao, Xu, Yanbing, Cheng, Chunzhen, Lü, Peitao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9570239/
https://www.ncbi.nlm.nih.gov/pubmed/36233216
http://dx.doi.org/10.3390/ijms231911914
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author Ma, Ronghui
Tian, Na
Wang, Jiashui
Fan, Minlei
Wang, Bin
Qu, Pengyan
Xu, Shiyao
Xu, Yanbing
Cheng, Chunzhen
Lü, Peitao
author_facet Ma, Ronghui
Tian, Na
Wang, Jiashui
Fan, Minlei
Wang, Bin
Qu, Pengyan
Xu, Shiyao
Xu, Yanbing
Cheng, Chunzhen
Lü, Peitao
author_sort Ma, Ronghui
collection PubMed
description Ca(2+)-ATPases have been confirmed to play very important roles in plant growth and development and in stress responses. However, studies on banana (Musa acuminata) Ca(2+)-ATPases are very limited. In this study, we identified 18 Ca(2+)-ATPase genes from banana, including 6 P-IIA or ER (Endoplasmic Reticulum) type Ca(2+)-ATPases (MaEACs) and 12 P-IIB or Auto-Inhibited Ca(2+)-ATPases (MaACAs). The MaEACs and MaACAs could be further classified into two and three subfamilies, respectively. This classification is well supported by their gene structures, which are encoded by protein motif distributions. The banana Ca(2+)-ATPases were all predicted to be plasma membrane-located. The promoter regions of banana Ca(2+)-ATPases contain many cis-acting elements and transcription factor binding sites (TFBS). A gene expression analysis showed that banana Ca(2+)-ATPases were differentially expressed in different organs. By investigating their expression patterns in banana roots under different concentrations of Ca(2+) treatments, we found that most banana Ca(2+)-ATPase members were highly expressed under 4 mM and 2 mM Ca(2+) treatments, but their expression decreased under 1 mM and 0 mM Ca(2+) treatments, suggesting that their downregulation might be closely related to reduced Ca accumulation and retarded growth under low Ca(2+) and Ca(2+) deficiency conditions. Our study will contribute to the understanding of the roles of Ca(2+)-ATPases in banana growth and Ca management.
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spelling pubmed-95702392022-10-17 Genome-Wide Identification and Characterization of Banana Ca(2+)-ATPase Genes and Expression Analysis under Different Concentrations of Ca(2+) Treatments Ma, Ronghui Tian, Na Wang, Jiashui Fan, Minlei Wang, Bin Qu, Pengyan Xu, Shiyao Xu, Yanbing Cheng, Chunzhen Lü, Peitao Int J Mol Sci Article Ca(2+)-ATPases have been confirmed to play very important roles in plant growth and development and in stress responses. However, studies on banana (Musa acuminata) Ca(2+)-ATPases are very limited. In this study, we identified 18 Ca(2+)-ATPase genes from banana, including 6 P-IIA or ER (Endoplasmic Reticulum) type Ca(2+)-ATPases (MaEACs) and 12 P-IIB or Auto-Inhibited Ca(2+)-ATPases (MaACAs). The MaEACs and MaACAs could be further classified into two and three subfamilies, respectively. This classification is well supported by their gene structures, which are encoded by protein motif distributions. The banana Ca(2+)-ATPases were all predicted to be plasma membrane-located. The promoter regions of banana Ca(2+)-ATPases contain many cis-acting elements and transcription factor binding sites (TFBS). A gene expression analysis showed that banana Ca(2+)-ATPases were differentially expressed in different organs. By investigating their expression patterns in banana roots under different concentrations of Ca(2+) treatments, we found that most banana Ca(2+)-ATPase members were highly expressed under 4 mM and 2 mM Ca(2+) treatments, but their expression decreased under 1 mM and 0 mM Ca(2+) treatments, suggesting that their downregulation might be closely related to reduced Ca accumulation and retarded growth under low Ca(2+) and Ca(2+) deficiency conditions. Our study will contribute to the understanding of the roles of Ca(2+)-ATPases in banana growth and Ca management. MDPI 2022-10-07 /pmc/articles/PMC9570239/ /pubmed/36233216 http://dx.doi.org/10.3390/ijms231911914 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ma, Ronghui
Tian, Na
Wang, Jiashui
Fan, Minlei
Wang, Bin
Qu, Pengyan
Xu, Shiyao
Xu, Yanbing
Cheng, Chunzhen
Lü, Peitao
Genome-Wide Identification and Characterization of Banana Ca(2+)-ATPase Genes and Expression Analysis under Different Concentrations of Ca(2+) Treatments
title Genome-Wide Identification and Characterization of Banana Ca(2+)-ATPase Genes and Expression Analysis under Different Concentrations of Ca(2+) Treatments
title_full Genome-Wide Identification and Characterization of Banana Ca(2+)-ATPase Genes and Expression Analysis under Different Concentrations of Ca(2+) Treatments
title_fullStr Genome-Wide Identification and Characterization of Banana Ca(2+)-ATPase Genes and Expression Analysis under Different Concentrations of Ca(2+) Treatments
title_full_unstemmed Genome-Wide Identification and Characterization of Banana Ca(2+)-ATPase Genes and Expression Analysis under Different Concentrations of Ca(2+) Treatments
title_short Genome-Wide Identification and Characterization of Banana Ca(2+)-ATPase Genes and Expression Analysis under Different Concentrations of Ca(2+) Treatments
title_sort genome-wide identification and characterization of banana ca(2+)-atpase genes and expression analysis under different concentrations of ca(2+) treatments
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9570239/
https://www.ncbi.nlm.nih.gov/pubmed/36233216
http://dx.doi.org/10.3390/ijms231911914
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