Cargando…

Cultivation of Cryopreserved Human Dental Pulp Stem Cells—A New Approach to Maintaining Dental Pulp Tissue

Human dental pulp stem cells (hDPSCs) are multipotent mesenchymal stem cells (MSCs) that are capable of self-renewal with multilineage differentiation potential. After being cryopreserved, hDPSCs were reported to maintain a high level of proliferation and multi-differentiation abilities. In order to...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Wang, Yan, Ming, Aarabi, Ghazal, Peters, Ulrike, Freytag, Marcus, Gosau, Martin, Smeets, Ralf, Beikler, Thomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9570360/
https://www.ncbi.nlm.nih.gov/pubmed/36232787
http://dx.doi.org/10.3390/ijms231911485
_version_ 1784810086923763712
author Wang, Wang
Yan, Ming
Aarabi, Ghazal
Peters, Ulrike
Freytag, Marcus
Gosau, Martin
Smeets, Ralf
Beikler, Thomas
author_facet Wang, Wang
Yan, Ming
Aarabi, Ghazal
Peters, Ulrike
Freytag, Marcus
Gosau, Martin
Smeets, Ralf
Beikler, Thomas
author_sort Wang, Wang
collection PubMed
description Human dental pulp stem cells (hDPSCs) are multipotent mesenchymal stem cells (MSCs) that are capable of self-renewal with multilineage differentiation potential. After being cryopreserved, hDPSCs were reported to maintain a high level of proliferation and multi-differentiation abilities. In order to optimize cryopreservation techniques, decrease storage requirements and lower contamination risks, the feasibility of new whole-tooth cryopreservation and its effects on hDPSCs were tested. The survival rates, morphology, proliferation rates, cell activity, surface antigens and differentiation abilities of hDPSCs isolated from fresh teeth were compared with those of one-month cryopreserved teeth in 5% and 10% DMSO. The data of the present study indicated that the new cryopreservation approach did not reduce the capabilities or stemness of hDPSCs, with the exception that it extended the first appearance time of hDPSCs in the teeth that were cryopreserved in 10% DMSO, and reduced their recovery rate. With the novel strategy of freezing, the hDPSCs still expressed the typical surface markers of MSCs and maintained excellent proliferation capacity. Three consecutive weeks of osteogenic and adipogenic induction also showed that the expression of the key genes in hDPSCs, including lipoprotein lipase (LPL), peroxisome proliferator-activated receptor-γ (PPAR-γ), alkaline phosphatase (ALP), runt-related transcription factor 2 (RUNX2), type I collagen (COL I) and osteocalcin (OSC) was not affected, indicating that their differentiation abilities remained intact, which are crucial parameters for hDPSCs as cell-therapy candidates. These results demonstrated that the new cryopreservation method is low-cost and effective for the good preservation of hDPSCs without compromising cell performance, and can provide ideas and evidence for the future application of stem-cell therapies and the establishment of dental banks.
format Online
Article
Text
id pubmed-9570360
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-95703602022-10-17 Cultivation of Cryopreserved Human Dental Pulp Stem Cells—A New Approach to Maintaining Dental Pulp Tissue Wang, Wang Yan, Ming Aarabi, Ghazal Peters, Ulrike Freytag, Marcus Gosau, Martin Smeets, Ralf Beikler, Thomas Int J Mol Sci Article Human dental pulp stem cells (hDPSCs) are multipotent mesenchymal stem cells (MSCs) that are capable of self-renewal with multilineage differentiation potential. After being cryopreserved, hDPSCs were reported to maintain a high level of proliferation and multi-differentiation abilities. In order to optimize cryopreservation techniques, decrease storage requirements and lower contamination risks, the feasibility of new whole-tooth cryopreservation and its effects on hDPSCs were tested. The survival rates, morphology, proliferation rates, cell activity, surface antigens and differentiation abilities of hDPSCs isolated from fresh teeth were compared with those of one-month cryopreserved teeth in 5% and 10% DMSO. The data of the present study indicated that the new cryopreservation approach did not reduce the capabilities or stemness of hDPSCs, with the exception that it extended the first appearance time of hDPSCs in the teeth that were cryopreserved in 10% DMSO, and reduced their recovery rate. With the novel strategy of freezing, the hDPSCs still expressed the typical surface markers of MSCs and maintained excellent proliferation capacity. Three consecutive weeks of osteogenic and adipogenic induction also showed that the expression of the key genes in hDPSCs, including lipoprotein lipase (LPL), peroxisome proliferator-activated receptor-γ (PPAR-γ), alkaline phosphatase (ALP), runt-related transcription factor 2 (RUNX2), type I collagen (COL I) and osteocalcin (OSC) was not affected, indicating that their differentiation abilities remained intact, which are crucial parameters for hDPSCs as cell-therapy candidates. These results demonstrated that the new cryopreservation method is low-cost and effective for the good preservation of hDPSCs without compromising cell performance, and can provide ideas and evidence for the future application of stem-cell therapies and the establishment of dental banks. MDPI 2022-09-29 /pmc/articles/PMC9570360/ /pubmed/36232787 http://dx.doi.org/10.3390/ijms231911485 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Wang
Yan, Ming
Aarabi, Ghazal
Peters, Ulrike
Freytag, Marcus
Gosau, Martin
Smeets, Ralf
Beikler, Thomas
Cultivation of Cryopreserved Human Dental Pulp Stem Cells—A New Approach to Maintaining Dental Pulp Tissue
title Cultivation of Cryopreserved Human Dental Pulp Stem Cells—A New Approach to Maintaining Dental Pulp Tissue
title_full Cultivation of Cryopreserved Human Dental Pulp Stem Cells—A New Approach to Maintaining Dental Pulp Tissue
title_fullStr Cultivation of Cryopreserved Human Dental Pulp Stem Cells—A New Approach to Maintaining Dental Pulp Tissue
title_full_unstemmed Cultivation of Cryopreserved Human Dental Pulp Stem Cells—A New Approach to Maintaining Dental Pulp Tissue
title_short Cultivation of Cryopreserved Human Dental Pulp Stem Cells—A New Approach to Maintaining Dental Pulp Tissue
title_sort cultivation of cryopreserved human dental pulp stem cells—a new approach to maintaining dental pulp tissue
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9570360/
https://www.ncbi.nlm.nih.gov/pubmed/36232787
http://dx.doi.org/10.3390/ijms231911485
work_keys_str_mv AT wangwang cultivationofcryopreservedhumandentalpulpstemcellsanewapproachtomaintainingdentalpulptissue
AT yanming cultivationofcryopreservedhumandentalpulpstemcellsanewapproachtomaintainingdentalpulptissue
AT aarabighazal cultivationofcryopreservedhumandentalpulpstemcellsanewapproachtomaintainingdentalpulptissue
AT petersulrike cultivationofcryopreservedhumandentalpulpstemcellsanewapproachtomaintainingdentalpulptissue
AT freytagmarcus cultivationofcryopreservedhumandentalpulpstemcellsanewapproachtomaintainingdentalpulptissue
AT gosaumartin cultivationofcryopreservedhumandentalpulpstemcellsanewapproachtomaintainingdentalpulptissue
AT smeetsralf cultivationofcryopreservedhumandentalpulpstemcellsanewapproachtomaintainingdentalpulptissue
AT beiklerthomas cultivationofcryopreservedhumandentalpulpstemcellsanewapproachtomaintainingdentalpulptissue