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Nuclear Functions of KaeA, a Subunit of the KEOPS Complex in Aspergillus nidulans
Kae1 is a subunit of the highly evolutionarily conserved KEOPS/EKC complex, which is involved in universal (t6A(37)) tRNA modification. Several reports have discussed the participation of this complex in transcription regulation in yeast and human cells, including our previous observations of KaeA,...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9570407/ https://www.ncbi.nlm.nih.gov/pubmed/36232439 http://dx.doi.org/10.3390/ijms231911138 |
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author | Gawlik, Joanna Koper, Michal Bogdanowicz, Albert Weglenski, Piotr Dzikowska, Agnieszka |
author_facet | Gawlik, Joanna Koper, Michal Bogdanowicz, Albert Weglenski, Piotr Dzikowska, Agnieszka |
author_sort | Gawlik, Joanna |
collection | PubMed |
description | Kae1 is a subunit of the highly evolutionarily conserved KEOPS/EKC complex, which is involved in universal (t6A(37)) tRNA modification. Several reports have discussed the participation of this complex in transcription regulation in yeast and human cells, including our previous observations of KaeA, an Aspergillus nidulans homologue of Kae1p. The aim of this project was to confirm the role of KaeA in transcription, employing high-throughput transcriptomic (RNA-Seq and ChIP-Seq) and proteomic (LC-MS) analysis. We confirmed that KaeA is a subunit of the KEOPS complex in A. nidulans. An analysis of kaeA19 and kaeA25 mutants showed that, although the (t6A(37)) tRNA modification is unaffected in both mutants, they reveal significantly altered transcriptomes compared to the wild type. The finding that KaeA is localized in chromatin and identifying its protein partners allows us to postulate an additional nuclear function for the protein. Our data shed light on the universal bi-functional role of this factor and proves that the activity of this protein is not limited to tRNA modification in cytoplasm, but also affects the transcriptional activity of a number of nuclear genes. Data are available via the NCBI’s GEO database under identifiers GSE206830 (RNA-Seq) and GSE206874 (ChIP-Seq), and via ProteomeXchange with identifier PXD034554 (proteomic). |
format | Online Article Text |
id | pubmed-9570407 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-95704072022-10-17 Nuclear Functions of KaeA, a Subunit of the KEOPS Complex in Aspergillus nidulans Gawlik, Joanna Koper, Michal Bogdanowicz, Albert Weglenski, Piotr Dzikowska, Agnieszka Int J Mol Sci Article Kae1 is a subunit of the highly evolutionarily conserved KEOPS/EKC complex, which is involved in universal (t6A(37)) tRNA modification. Several reports have discussed the participation of this complex in transcription regulation in yeast and human cells, including our previous observations of KaeA, an Aspergillus nidulans homologue of Kae1p. The aim of this project was to confirm the role of KaeA in transcription, employing high-throughput transcriptomic (RNA-Seq and ChIP-Seq) and proteomic (LC-MS) analysis. We confirmed that KaeA is a subunit of the KEOPS complex in A. nidulans. An analysis of kaeA19 and kaeA25 mutants showed that, although the (t6A(37)) tRNA modification is unaffected in both mutants, they reveal significantly altered transcriptomes compared to the wild type. The finding that KaeA is localized in chromatin and identifying its protein partners allows us to postulate an additional nuclear function for the protein. Our data shed light on the universal bi-functional role of this factor and proves that the activity of this protein is not limited to tRNA modification in cytoplasm, but also affects the transcriptional activity of a number of nuclear genes. Data are available via the NCBI’s GEO database under identifiers GSE206830 (RNA-Seq) and GSE206874 (ChIP-Seq), and via ProteomeXchange with identifier PXD034554 (proteomic). MDPI 2022-09-22 /pmc/articles/PMC9570407/ /pubmed/36232439 http://dx.doi.org/10.3390/ijms231911138 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Gawlik, Joanna Koper, Michal Bogdanowicz, Albert Weglenski, Piotr Dzikowska, Agnieszka Nuclear Functions of KaeA, a Subunit of the KEOPS Complex in Aspergillus nidulans |
title | Nuclear Functions of KaeA, a Subunit of the KEOPS Complex in Aspergillus nidulans |
title_full | Nuclear Functions of KaeA, a Subunit of the KEOPS Complex in Aspergillus nidulans |
title_fullStr | Nuclear Functions of KaeA, a Subunit of the KEOPS Complex in Aspergillus nidulans |
title_full_unstemmed | Nuclear Functions of KaeA, a Subunit of the KEOPS Complex in Aspergillus nidulans |
title_short | Nuclear Functions of KaeA, a Subunit of the KEOPS Complex in Aspergillus nidulans |
title_sort | nuclear functions of kaea, a subunit of the keops complex in aspergillus nidulans |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9570407/ https://www.ncbi.nlm.nih.gov/pubmed/36232439 http://dx.doi.org/10.3390/ijms231911138 |
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