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Diversity of Extracellular Vesicles in Human Follicular Fluid: Morphological Analysis and Quantification

The oocyte microenvironment constituted by the follicular fluid (FF) is a key for the optimal development of female gametes. Its composition reflects the physiological state of the ovarian follicle. The particularity of FF is to contain a huge diversity of extracellular vesicles specific to women, i...

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Autores principales: Neyroud, Anne-Sophie, Chiechio, Regina Maria, Moulin, Gregory, Ducarre, Solène, Heichette, Claire, Dupont, Aurélien, Budzynski, Mathieu, Even-Hernandez, Pascale, Faro, Maria Jose Lo, Yefimova, Marina, Marchi, Valérie, Ravel, Célia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9570429/
https://www.ncbi.nlm.nih.gov/pubmed/36232981
http://dx.doi.org/10.3390/ijms231911676
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author Neyroud, Anne-Sophie
Chiechio, Regina Maria
Moulin, Gregory
Ducarre, Solène
Heichette, Claire
Dupont, Aurélien
Budzynski, Mathieu
Even-Hernandez, Pascale
Faro, Maria Jose Lo
Yefimova, Marina
Marchi, Valérie
Ravel, Célia
author_facet Neyroud, Anne-Sophie
Chiechio, Regina Maria
Moulin, Gregory
Ducarre, Solène
Heichette, Claire
Dupont, Aurélien
Budzynski, Mathieu
Even-Hernandez, Pascale
Faro, Maria Jose Lo
Yefimova, Marina
Marchi, Valérie
Ravel, Célia
author_sort Neyroud, Anne-Sophie
collection PubMed
description The oocyte microenvironment constituted by the follicular fluid (FF) is a key for the optimal development of female gametes. Its composition reflects the physiological state of the ovarian follicle. The particularity of FF is to contain a huge diversity of extracellular vesicles specific to women, in the same way as seminal plasma in men. Here, we described and compared morphological aspects of broad subcategories of human FF-related Extracellular Vesicles (EVs). EVs participate in physiological and pathological processes and have potential applications in diagnostics or therapeutics. EVs isolated from FF are involved in different biological functions related to follicular growth, oocyte maturation, and embryo development. However, knowledge on the morphology of FF-derived EVs is limited, mainly due to their sub-micrometer size and to intrinsic limitations in methods applied for their characterization. The aim of this study was to provide a comprehensive morphological description of EVs from FF of healthy subjects and quantification. EVs separation was realized by centrifugation, with comparison of the EV yield obtained from differential centrifugation and one-step ultracentrifugation. Cryo-Transmission Electron Microscopy was used to reveal the morphology, size, and phenotype of EVs. Dynamic Light Scattering (DLS) and Nanoparticle Tracking Analysis (NTA) were used to quantify and analyze the size distribution for each centrifugation step. We performed a comprehensive inventory of human follicular fluid EVs. We show that human FF contains a huge diversity of EVs. This study brings novel insights on EVs from normal FF and provides a reference for further studies of EVs in ovarian diseases.
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spelling pubmed-95704292022-10-17 Diversity of Extracellular Vesicles in Human Follicular Fluid: Morphological Analysis and Quantification Neyroud, Anne-Sophie Chiechio, Regina Maria Moulin, Gregory Ducarre, Solène Heichette, Claire Dupont, Aurélien Budzynski, Mathieu Even-Hernandez, Pascale Faro, Maria Jose Lo Yefimova, Marina Marchi, Valérie Ravel, Célia Int J Mol Sci Article The oocyte microenvironment constituted by the follicular fluid (FF) is a key for the optimal development of female gametes. Its composition reflects the physiological state of the ovarian follicle. The particularity of FF is to contain a huge diversity of extracellular vesicles specific to women, in the same way as seminal plasma in men. Here, we described and compared morphological aspects of broad subcategories of human FF-related Extracellular Vesicles (EVs). EVs participate in physiological and pathological processes and have potential applications in diagnostics or therapeutics. EVs isolated from FF are involved in different biological functions related to follicular growth, oocyte maturation, and embryo development. However, knowledge on the morphology of FF-derived EVs is limited, mainly due to their sub-micrometer size and to intrinsic limitations in methods applied for their characterization. The aim of this study was to provide a comprehensive morphological description of EVs from FF of healthy subjects and quantification. EVs separation was realized by centrifugation, with comparison of the EV yield obtained from differential centrifugation and one-step ultracentrifugation. Cryo-Transmission Electron Microscopy was used to reveal the morphology, size, and phenotype of EVs. Dynamic Light Scattering (DLS) and Nanoparticle Tracking Analysis (NTA) were used to quantify and analyze the size distribution for each centrifugation step. We performed a comprehensive inventory of human follicular fluid EVs. We show that human FF contains a huge diversity of EVs. This study brings novel insights on EVs from normal FF and provides a reference for further studies of EVs in ovarian diseases. MDPI 2022-10-02 /pmc/articles/PMC9570429/ /pubmed/36232981 http://dx.doi.org/10.3390/ijms231911676 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Neyroud, Anne-Sophie
Chiechio, Regina Maria
Moulin, Gregory
Ducarre, Solène
Heichette, Claire
Dupont, Aurélien
Budzynski, Mathieu
Even-Hernandez, Pascale
Faro, Maria Jose Lo
Yefimova, Marina
Marchi, Valérie
Ravel, Célia
Diversity of Extracellular Vesicles in Human Follicular Fluid: Morphological Analysis and Quantification
title Diversity of Extracellular Vesicles in Human Follicular Fluid: Morphological Analysis and Quantification
title_full Diversity of Extracellular Vesicles in Human Follicular Fluid: Morphological Analysis and Quantification
title_fullStr Diversity of Extracellular Vesicles in Human Follicular Fluid: Morphological Analysis and Quantification
title_full_unstemmed Diversity of Extracellular Vesicles in Human Follicular Fluid: Morphological Analysis and Quantification
title_short Diversity of Extracellular Vesicles in Human Follicular Fluid: Morphological Analysis and Quantification
title_sort diversity of extracellular vesicles in human follicular fluid: morphological analysis and quantification
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9570429/
https://www.ncbi.nlm.nih.gov/pubmed/36232981
http://dx.doi.org/10.3390/ijms231911676
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