Cargando…
Diversity of Extracellular Vesicles in Human Follicular Fluid: Morphological Analysis and Quantification
The oocyte microenvironment constituted by the follicular fluid (FF) is a key for the optimal development of female gametes. Its composition reflects the physiological state of the ovarian follicle. The particularity of FF is to contain a huge diversity of extracellular vesicles specific to women, i...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9570429/ https://www.ncbi.nlm.nih.gov/pubmed/36232981 http://dx.doi.org/10.3390/ijms231911676 |
_version_ | 1784810104288182272 |
---|---|
author | Neyroud, Anne-Sophie Chiechio, Regina Maria Moulin, Gregory Ducarre, Solène Heichette, Claire Dupont, Aurélien Budzynski, Mathieu Even-Hernandez, Pascale Faro, Maria Jose Lo Yefimova, Marina Marchi, Valérie Ravel, Célia |
author_facet | Neyroud, Anne-Sophie Chiechio, Regina Maria Moulin, Gregory Ducarre, Solène Heichette, Claire Dupont, Aurélien Budzynski, Mathieu Even-Hernandez, Pascale Faro, Maria Jose Lo Yefimova, Marina Marchi, Valérie Ravel, Célia |
author_sort | Neyroud, Anne-Sophie |
collection | PubMed |
description | The oocyte microenvironment constituted by the follicular fluid (FF) is a key for the optimal development of female gametes. Its composition reflects the physiological state of the ovarian follicle. The particularity of FF is to contain a huge diversity of extracellular vesicles specific to women, in the same way as seminal plasma in men. Here, we described and compared morphological aspects of broad subcategories of human FF-related Extracellular Vesicles (EVs). EVs participate in physiological and pathological processes and have potential applications in diagnostics or therapeutics. EVs isolated from FF are involved in different biological functions related to follicular growth, oocyte maturation, and embryo development. However, knowledge on the morphology of FF-derived EVs is limited, mainly due to their sub-micrometer size and to intrinsic limitations in methods applied for their characterization. The aim of this study was to provide a comprehensive morphological description of EVs from FF of healthy subjects and quantification. EVs separation was realized by centrifugation, with comparison of the EV yield obtained from differential centrifugation and one-step ultracentrifugation. Cryo-Transmission Electron Microscopy was used to reveal the morphology, size, and phenotype of EVs. Dynamic Light Scattering (DLS) and Nanoparticle Tracking Analysis (NTA) were used to quantify and analyze the size distribution for each centrifugation step. We performed a comprehensive inventory of human follicular fluid EVs. We show that human FF contains a huge diversity of EVs. This study brings novel insights on EVs from normal FF and provides a reference for further studies of EVs in ovarian diseases. |
format | Online Article Text |
id | pubmed-9570429 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-95704292022-10-17 Diversity of Extracellular Vesicles in Human Follicular Fluid: Morphological Analysis and Quantification Neyroud, Anne-Sophie Chiechio, Regina Maria Moulin, Gregory Ducarre, Solène Heichette, Claire Dupont, Aurélien Budzynski, Mathieu Even-Hernandez, Pascale Faro, Maria Jose Lo Yefimova, Marina Marchi, Valérie Ravel, Célia Int J Mol Sci Article The oocyte microenvironment constituted by the follicular fluid (FF) is a key for the optimal development of female gametes. Its composition reflects the physiological state of the ovarian follicle. The particularity of FF is to contain a huge diversity of extracellular vesicles specific to women, in the same way as seminal plasma in men. Here, we described and compared morphological aspects of broad subcategories of human FF-related Extracellular Vesicles (EVs). EVs participate in physiological and pathological processes and have potential applications in diagnostics or therapeutics. EVs isolated from FF are involved in different biological functions related to follicular growth, oocyte maturation, and embryo development. However, knowledge on the morphology of FF-derived EVs is limited, mainly due to their sub-micrometer size and to intrinsic limitations in methods applied for their characterization. The aim of this study was to provide a comprehensive morphological description of EVs from FF of healthy subjects and quantification. EVs separation was realized by centrifugation, with comparison of the EV yield obtained from differential centrifugation and one-step ultracentrifugation. Cryo-Transmission Electron Microscopy was used to reveal the morphology, size, and phenotype of EVs. Dynamic Light Scattering (DLS) and Nanoparticle Tracking Analysis (NTA) were used to quantify and analyze the size distribution for each centrifugation step. We performed a comprehensive inventory of human follicular fluid EVs. We show that human FF contains a huge diversity of EVs. This study brings novel insights on EVs from normal FF and provides a reference for further studies of EVs in ovarian diseases. MDPI 2022-10-02 /pmc/articles/PMC9570429/ /pubmed/36232981 http://dx.doi.org/10.3390/ijms231911676 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Neyroud, Anne-Sophie Chiechio, Regina Maria Moulin, Gregory Ducarre, Solène Heichette, Claire Dupont, Aurélien Budzynski, Mathieu Even-Hernandez, Pascale Faro, Maria Jose Lo Yefimova, Marina Marchi, Valérie Ravel, Célia Diversity of Extracellular Vesicles in Human Follicular Fluid: Morphological Analysis and Quantification |
title | Diversity of Extracellular Vesicles in Human Follicular Fluid: Morphological Analysis and Quantification |
title_full | Diversity of Extracellular Vesicles in Human Follicular Fluid: Morphological Analysis and Quantification |
title_fullStr | Diversity of Extracellular Vesicles in Human Follicular Fluid: Morphological Analysis and Quantification |
title_full_unstemmed | Diversity of Extracellular Vesicles in Human Follicular Fluid: Morphological Analysis and Quantification |
title_short | Diversity of Extracellular Vesicles in Human Follicular Fluid: Morphological Analysis and Quantification |
title_sort | diversity of extracellular vesicles in human follicular fluid: morphological analysis and quantification |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9570429/ https://www.ncbi.nlm.nih.gov/pubmed/36232981 http://dx.doi.org/10.3390/ijms231911676 |
work_keys_str_mv | AT neyroudannesophie diversityofextracellularvesiclesinhumanfollicularfluidmorphologicalanalysisandquantification AT chiechioreginamaria diversityofextracellularvesiclesinhumanfollicularfluidmorphologicalanalysisandquantification AT moulingregory diversityofextracellularvesiclesinhumanfollicularfluidmorphologicalanalysisandquantification AT ducarresolene diversityofextracellularvesiclesinhumanfollicularfluidmorphologicalanalysisandquantification AT heichetteclaire diversityofextracellularvesiclesinhumanfollicularfluidmorphologicalanalysisandquantification AT dupontaurelien diversityofextracellularvesiclesinhumanfollicularfluidmorphologicalanalysisandquantification AT budzynskimathieu diversityofextracellularvesiclesinhumanfollicularfluidmorphologicalanalysisandquantification AT evenhernandezpascale diversityofextracellularvesiclesinhumanfollicularfluidmorphologicalanalysisandquantification AT faromariajoselo diversityofextracellularvesiclesinhumanfollicularfluidmorphologicalanalysisandquantification AT yefimovamarina diversityofextracellularvesiclesinhumanfollicularfluidmorphologicalanalysisandquantification AT marchivalerie diversityofextracellularvesiclesinhumanfollicularfluidmorphologicalanalysisandquantification AT ravelcelia diversityofextracellularvesiclesinhumanfollicularfluidmorphologicalanalysisandquantification |