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Tumor-Informed Approach Improved ctDNA Detection Rate in Resected Pancreatic Cancer

Pancreatic cancer is one of the cancers with very poor prognosis; there is an urgent need to identify novel biomarkers to improve its clinical outcomes. Circulating tumor DNA (ctDNA) from liquid biopsy has arisen as a promising biomarker for cancer detection and surveillance. However, it is known th...

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Autores principales: Watanabe, Kazunori, Nakamura, Toru, Kimura, Yasutoshi, Motoya, Masayo, Kojima, Shigeyuki, Kuraya, Tomotaka, Murakami, Takeshi, Kaneko, Tsukasa, Shinohara, Yoshihito, Kitayama, Yosuke, Fukuda, Keito, Hatanaka, Kanako C., Mitsuhashi, Tomoko, Pittella-Silva, Fabio, Yamaguchi, Toshikazu, Hirano, Satoshi, Nakamura, Yusuke, Low, Siew-Kee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9570468/
https://www.ncbi.nlm.nih.gov/pubmed/36232820
http://dx.doi.org/10.3390/ijms231911521
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author Watanabe, Kazunori
Nakamura, Toru
Kimura, Yasutoshi
Motoya, Masayo
Kojima, Shigeyuki
Kuraya, Tomotaka
Murakami, Takeshi
Kaneko, Tsukasa
Shinohara, Yoshihito
Kitayama, Yosuke
Fukuda, Keito
Hatanaka, Kanako C.
Mitsuhashi, Tomoko
Pittella-Silva, Fabio
Yamaguchi, Toshikazu
Hirano, Satoshi
Nakamura, Yusuke
Low, Siew-Kee
author_facet Watanabe, Kazunori
Nakamura, Toru
Kimura, Yasutoshi
Motoya, Masayo
Kojima, Shigeyuki
Kuraya, Tomotaka
Murakami, Takeshi
Kaneko, Tsukasa
Shinohara, Yoshihito
Kitayama, Yosuke
Fukuda, Keito
Hatanaka, Kanako C.
Mitsuhashi, Tomoko
Pittella-Silva, Fabio
Yamaguchi, Toshikazu
Hirano, Satoshi
Nakamura, Yusuke
Low, Siew-Kee
author_sort Watanabe, Kazunori
collection PubMed
description Pancreatic cancer is one of the cancers with very poor prognosis; there is an urgent need to identify novel biomarkers to improve its clinical outcomes. Circulating tumor DNA (ctDNA) from liquid biopsy has arisen as a promising biomarker for cancer detection and surveillance. However, it is known that the ctDNA detection rate in resected pancreatic cancer is low compared with other types of cancer. In this study, we collected paired tumor and plasma samples from 145 pancreatic cancer patients. Plasma samples were collected from 71 patients of treatment-naïve status and from 74 patients after neoadjuvant therapy (NAT). Genomic profiling of tumor DNA and plasma samples was conducted using targeted next-generation sequencing (NGS). Somatic mutations were detected in 85% (123/145) of tumors. ctDNA was detected in 39% (28/71) and 31% (23/74) of treatment-naïve and after-NAT groups, respectively, without referring to the information of tumor profiles. With a tumor-informed approach (TIA), ctDNA detection rate improved to 56% (40/71) and 36% (27/74) in treatment-naïve and after-NAT groups, respectively, with the detection rate significantly improved (p = 0.0165) among the treatment-naïve group compared to the after-NAT group. Cases who had detectable plasma ctDNA concordant to the corresponding tumor showed significantly shorter recurrence-free survival (RFS) (p = 0.0010). We demonstrated that TIA improves ctDNA detection rate in pancreatic cancer, and that ctDNA could be a potential prognostic biomarker for recurrence risk prediction
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spelling pubmed-95704682022-10-17 Tumor-Informed Approach Improved ctDNA Detection Rate in Resected Pancreatic Cancer Watanabe, Kazunori Nakamura, Toru Kimura, Yasutoshi Motoya, Masayo Kojima, Shigeyuki Kuraya, Tomotaka Murakami, Takeshi Kaneko, Tsukasa Shinohara, Yoshihito Kitayama, Yosuke Fukuda, Keito Hatanaka, Kanako C. Mitsuhashi, Tomoko Pittella-Silva, Fabio Yamaguchi, Toshikazu Hirano, Satoshi Nakamura, Yusuke Low, Siew-Kee Int J Mol Sci Article Pancreatic cancer is one of the cancers with very poor prognosis; there is an urgent need to identify novel biomarkers to improve its clinical outcomes. Circulating tumor DNA (ctDNA) from liquid biopsy has arisen as a promising biomarker for cancer detection and surveillance. However, it is known that the ctDNA detection rate in resected pancreatic cancer is low compared with other types of cancer. In this study, we collected paired tumor and plasma samples from 145 pancreatic cancer patients. Plasma samples were collected from 71 patients of treatment-naïve status and from 74 patients after neoadjuvant therapy (NAT). Genomic profiling of tumor DNA and plasma samples was conducted using targeted next-generation sequencing (NGS). Somatic mutations were detected in 85% (123/145) of tumors. ctDNA was detected in 39% (28/71) and 31% (23/74) of treatment-naïve and after-NAT groups, respectively, without referring to the information of tumor profiles. With a tumor-informed approach (TIA), ctDNA detection rate improved to 56% (40/71) and 36% (27/74) in treatment-naïve and after-NAT groups, respectively, with the detection rate significantly improved (p = 0.0165) among the treatment-naïve group compared to the after-NAT group. Cases who had detectable plasma ctDNA concordant to the corresponding tumor showed significantly shorter recurrence-free survival (RFS) (p = 0.0010). We demonstrated that TIA improves ctDNA detection rate in pancreatic cancer, and that ctDNA could be a potential prognostic biomarker for recurrence risk prediction MDPI 2022-09-29 /pmc/articles/PMC9570468/ /pubmed/36232820 http://dx.doi.org/10.3390/ijms231911521 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Watanabe, Kazunori
Nakamura, Toru
Kimura, Yasutoshi
Motoya, Masayo
Kojima, Shigeyuki
Kuraya, Tomotaka
Murakami, Takeshi
Kaneko, Tsukasa
Shinohara, Yoshihito
Kitayama, Yosuke
Fukuda, Keito
Hatanaka, Kanako C.
Mitsuhashi, Tomoko
Pittella-Silva, Fabio
Yamaguchi, Toshikazu
Hirano, Satoshi
Nakamura, Yusuke
Low, Siew-Kee
Tumor-Informed Approach Improved ctDNA Detection Rate in Resected Pancreatic Cancer
title Tumor-Informed Approach Improved ctDNA Detection Rate in Resected Pancreatic Cancer
title_full Tumor-Informed Approach Improved ctDNA Detection Rate in Resected Pancreatic Cancer
title_fullStr Tumor-Informed Approach Improved ctDNA Detection Rate in Resected Pancreatic Cancer
title_full_unstemmed Tumor-Informed Approach Improved ctDNA Detection Rate in Resected Pancreatic Cancer
title_short Tumor-Informed Approach Improved ctDNA Detection Rate in Resected Pancreatic Cancer
title_sort tumor-informed approach improved ctdna detection rate in resected pancreatic cancer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9570468/
https://www.ncbi.nlm.nih.gov/pubmed/36232820
http://dx.doi.org/10.3390/ijms231911521
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