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Microsporum gypseum Isolated from Ailuropoda melanoleuca Provokes Inflammation and Triggers Th17 Adaptive Immunity Response
Microsporum gypseum causes dermatomycoses in giant pandas (Ailuropoda melanoleuca). This study aimed to investigate the immune response of M. gypseum following deep infection. The degree of damage to the heart, liver, spleen, lungs, and kidneys was evaluated using tissue fungal load, organ index, an...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9570494/ https://www.ncbi.nlm.nih.gov/pubmed/36233337 http://dx.doi.org/10.3390/ijms231912037 |
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author | Ma, Xiaoping Liu, Zhen Yu, Yan Jiang, Yaozhang Wang, Chengdong Zuo, Zhicai Ling, Shanshan He, Ming Cao, Sanjie Wen, Yiping Zhao, Qin Wu, Rui Huang, Xiaobo Zhong, Zhijun Peng, Guangneng Gu, Yu |
author_facet | Ma, Xiaoping Liu, Zhen Yu, Yan Jiang, Yaozhang Wang, Chengdong Zuo, Zhicai Ling, Shanshan He, Ming Cao, Sanjie Wen, Yiping Zhao, Qin Wu, Rui Huang, Xiaobo Zhong, Zhijun Peng, Guangneng Gu, Yu |
author_sort | Ma, Xiaoping |
collection | PubMed |
description | Microsporum gypseum causes dermatomycoses in giant pandas (Ailuropoda melanoleuca). This study aimed to investigate the immune response of M. gypseum following deep infection. The degree of damage to the heart, liver, spleen, lungs, and kidneys was evaluated using tissue fungal load, organ index, and histopathological methods. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) detected the mRNA expression of receptors and cytokines in the lung, and immunofluorescence staining and flow cytometry, were used to assess immune cells in the lung. The results indicated that conidia mainly colonized the lungs and caused serious injury with M. gypseum infection. Furthermore, dectin-1, TLR-2, and TLR-4 played a role in recognizing M. gypseum cells. Numerous inflammatory cells, mainly macrophages, dendritic cells, polymorphonuclear neutrophils, and inflammatory cytokines (TGF-β, TNF-α, IL-1β, IL-6, IL-10, IL-12, and IL-23), were activated in the early stages of infection. With the high expression of IL-22, IL-17A, and IL-17F, the Th17 pathway exerted an adaptive immune response to M. gypseum infection. These results can potentially aid in the diagnosis and treatment of diseases caused by M. gypseum in giant pandas. |
format | Online Article Text |
id | pubmed-9570494 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-95704942022-10-17 Microsporum gypseum Isolated from Ailuropoda melanoleuca Provokes Inflammation and Triggers Th17 Adaptive Immunity Response Ma, Xiaoping Liu, Zhen Yu, Yan Jiang, Yaozhang Wang, Chengdong Zuo, Zhicai Ling, Shanshan He, Ming Cao, Sanjie Wen, Yiping Zhao, Qin Wu, Rui Huang, Xiaobo Zhong, Zhijun Peng, Guangneng Gu, Yu Int J Mol Sci Article Microsporum gypseum causes dermatomycoses in giant pandas (Ailuropoda melanoleuca). This study aimed to investigate the immune response of M. gypseum following deep infection. The degree of damage to the heart, liver, spleen, lungs, and kidneys was evaluated using tissue fungal load, organ index, and histopathological methods. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) detected the mRNA expression of receptors and cytokines in the lung, and immunofluorescence staining and flow cytometry, were used to assess immune cells in the lung. The results indicated that conidia mainly colonized the lungs and caused serious injury with M. gypseum infection. Furthermore, dectin-1, TLR-2, and TLR-4 played a role in recognizing M. gypseum cells. Numerous inflammatory cells, mainly macrophages, dendritic cells, polymorphonuclear neutrophils, and inflammatory cytokines (TGF-β, TNF-α, IL-1β, IL-6, IL-10, IL-12, and IL-23), were activated in the early stages of infection. With the high expression of IL-22, IL-17A, and IL-17F, the Th17 pathway exerted an adaptive immune response to M. gypseum infection. These results can potentially aid in the diagnosis and treatment of diseases caused by M. gypseum in giant pandas. MDPI 2022-10-10 /pmc/articles/PMC9570494/ /pubmed/36233337 http://dx.doi.org/10.3390/ijms231912037 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ma, Xiaoping Liu, Zhen Yu, Yan Jiang, Yaozhang Wang, Chengdong Zuo, Zhicai Ling, Shanshan He, Ming Cao, Sanjie Wen, Yiping Zhao, Qin Wu, Rui Huang, Xiaobo Zhong, Zhijun Peng, Guangneng Gu, Yu Microsporum gypseum Isolated from Ailuropoda melanoleuca Provokes Inflammation and Triggers Th17 Adaptive Immunity Response |
title | Microsporum gypseum Isolated from Ailuropoda melanoleuca Provokes Inflammation and Triggers Th17 Adaptive Immunity Response |
title_full | Microsporum gypseum Isolated from Ailuropoda melanoleuca Provokes Inflammation and Triggers Th17 Adaptive Immunity Response |
title_fullStr | Microsporum gypseum Isolated from Ailuropoda melanoleuca Provokes Inflammation and Triggers Th17 Adaptive Immunity Response |
title_full_unstemmed | Microsporum gypseum Isolated from Ailuropoda melanoleuca Provokes Inflammation and Triggers Th17 Adaptive Immunity Response |
title_short | Microsporum gypseum Isolated from Ailuropoda melanoleuca Provokes Inflammation and Triggers Th17 Adaptive Immunity Response |
title_sort | microsporum gypseum isolated from ailuropoda melanoleuca provokes inflammation and triggers th17 adaptive immunity response |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9570494/ https://www.ncbi.nlm.nih.gov/pubmed/36233337 http://dx.doi.org/10.3390/ijms231912037 |
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