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Microsporum gypseum Isolated from Ailuropoda melanoleuca Provokes Inflammation and Triggers Th17 Adaptive Immunity Response

Microsporum gypseum causes dermatomycoses in giant pandas (Ailuropoda melanoleuca). This study aimed to investigate the immune response of M. gypseum following deep infection. The degree of damage to the heart, liver, spleen, lungs, and kidneys was evaluated using tissue fungal load, organ index, an...

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Autores principales: Ma, Xiaoping, Liu, Zhen, Yu, Yan, Jiang, Yaozhang, Wang, Chengdong, Zuo, Zhicai, Ling, Shanshan, He, Ming, Cao, Sanjie, Wen, Yiping, Zhao, Qin, Wu, Rui, Huang, Xiaobo, Zhong, Zhijun, Peng, Guangneng, Gu, Yu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9570494/
https://www.ncbi.nlm.nih.gov/pubmed/36233337
http://dx.doi.org/10.3390/ijms231912037
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author Ma, Xiaoping
Liu, Zhen
Yu, Yan
Jiang, Yaozhang
Wang, Chengdong
Zuo, Zhicai
Ling, Shanshan
He, Ming
Cao, Sanjie
Wen, Yiping
Zhao, Qin
Wu, Rui
Huang, Xiaobo
Zhong, Zhijun
Peng, Guangneng
Gu, Yu
author_facet Ma, Xiaoping
Liu, Zhen
Yu, Yan
Jiang, Yaozhang
Wang, Chengdong
Zuo, Zhicai
Ling, Shanshan
He, Ming
Cao, Sanjie
Wen, Yiping
Zhao, Qin
Wu, Rui
Huang, Xiaobo
Zhong, Zhijun
Peng, Guangneng
Gu, Yu
author_sort Ma, Xiaoping
collection PubMed
description Microsporum gypseum causes dermatomycoses in giant pandas (Ailuropoda melanoleuca). This study aimed to investigate the immune response of M. gypseum following deep infection. The degree of damage to the heart, liver, spleen, lungs, and kidneys was evaluated using tissue fungal load, organ index, and histopathological methods. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) detected the mRNA expression of receptors and cytokines in the lung, and immunofluorescence staining and flow cytometry, were used to assess immune cells in the lung. The results indicated that conidia mainly colonized the lungs and caused serious injury with M. gypseum infection. Furthermore, dectin-1, TLR-2, and TLR-4 played a role in recognizing M. gypseum cells. Numerous inflammatory cells, mainly macrophages, dendritic cells, polymorphonuclear neutrophils, and inflammatory cytokines (TGF-β, TNF-α, IL-1β, IL-6, IL-10, IL-12, and IL-23), were activated in the early stages of infection. With the high expression of IL-22, IL-17A, and IL-17F, the Th17 pathway exerted an adaptive immune response to M. gypseum infection. These results can potentially aid in the diagnosis and treatment of diseases caused by M. gypseum in giant pandas.
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spelling pubmed-95704942022-10-17 Microsporum gypseum Isolated from Ailuropoda melanoleuca Provokes Inflammation and Triggers Th17 Adaptive Immunity Response Ma, Xiaoping Liu, Zhen Yu, Yan Jiang, Yaozhang Wang, Chengdong Zuo, Zhicai Ling, Shanshan He, Ming Cao, Sanjie Wen, Yiping Zhao, Qin Wu, Rui Huang, Xiaobo Zhong, Zhijun Peng, Guangneng Gu, Yu Int J Mol Sci Article Microsporum gypseum causes dermatomycoses in giant pandas (Ailuropoda melanoleuca). This study aimed to investigate the immune response of M. gypseum following deep infection. The degree of damage to the heart, liver, spleen, lungs, and kidneys was evaluated using tissue fungal load, organ index, and histopathological methods. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) detected the mRNA expression of receptors and cytokines in the lung, and immunofluorescence staining and flow cytometry, were used to assess immune cells in the lung. The results indicated that conidia mainly colonized the lungs and caused serious injury with M. gypseum infection. Furthermore, dectin-1, TLR-2, and TLR-4 played a role in recognizing M. gypseum cells. Numerous inflammatory cells, mainly macrophages, dendritic cells, polymorphonuclear neutrophils, and inflammatory cytokines (TGF-β, TNF-α, IL-1β, IL-6, IL-10, IL-12, and IL-23), were activated in the early stages of infection. With the high expression of IL-22, IL-17A, and IL-17F, the Th17 pathway exerted an adaptive immune response to M. gypseum infection. These results can potentially aid in the diagnosis and treatment of diseases caused by M. gypseum in giant pandas. MDPI 2022-10-10 /pmc/articles/PMC9570494/ /pubmed/36233337 http://dx.doi.org/10.3390/ijms231912037 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ma, Xiaoping
Liu, Zhen
Yu, Yan
Jiang, Yaozhang
Wang, Chengdong
Zuo, Zhicai
Ling, Shanshan
He, Ming
Cao, Sanjie
Wen, Yiping
Zhao, Qin
Wu, Rui
Huang, Xiaobo
Zhong, Zhijun
Peng, Guangneng
Gu, Yu
Microsporum gypseum Isolated from Ailuropoda melanoleuca Provokes Inflammation and Triggers Th17 Adaptive Immunity Response
title Microsporum gypseum Isolated from Ailuropoda melanoleuca Provokes Inflammation and Triggers Th17 Adaptive Immunity Response
title_full Microsporum gypseum Isolated from Ailuropoda melanoleuca Provokes Inflammation and Triggers Th17 Adaptive Immunity Response
title_fullStr Microsporum gypseum Isolated from Ailuropoda melanoleuca Provokes Inflammation and Triggers Th17 Adaptive Immunity Response
title_full_unstemmed Microsporum gypseum Isolated from Ailuropoda melanoleuca Provokes Inflammation and Triggers Th17 Adaptive Immunity Response
title_short Microsporum gypseum Isolated from Ailuropoda melanoleuca Provokes Inflammation and Triggers Th17 Adaptive Immunity Response
title_sort microsporum gypseum isolated from ailuropoda melanoleuca provokes inflammation and triggers th17 adaptive immunity response
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9570494/
https://www.ncbi.nlm.nih.gov/pubmed/36233337
http://dx.doi.org/10.3390/ijms231912037
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