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High-Risk Human Papillomavirus (HR-HPV) DNA Detection in Mouthwashes for Diagnosis of HPV-Driven Oropharynx Cancer and Its Curative Therapy—A Feasibility Study

Detection of p16 through immunohistochemistry (IHC) is the standard for determining the HPV status of the tumor according the TNM eighth edition released in 2017 and has become crucial for determining the HPV status of oropharyngeal squamous cell carcinomas (OPSCC) with direct impact on staging and...

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Autores principales: Loermann, Gera, Kolb, Marlen, Prascevic, Dusan, Siemert, Julia, Wiegand, Susanne, Zebralla, Veit, Pirlich, Markus, Stöhr, Matthäus, Dietz, Andreas, Wald, Theresa, Wichmann, Gunnar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9570661/
https://www.ncbi.nlm.nih.gov/pubmed/36233378
http://dx.doi.org/10.3390/jcm11195509
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author Loermann, Gera
Kolb, Marlen
Prascevic, Dusan
Siemert, Julia
Wiegand, Susanne
Zebralla, Veit
Pirlich, Markus
Stöhr, Matthäus
Dietz, Andreas
Wald, Theresa
Wichmann, Gunnar
author_facet Loermann, Gera
Kolb, Marlen
Prascevic, Dusan
Siemert, Julia
Wiegand, Susanne
Zebralla, Veit
Pirlich, Markus
Stöhr, Matthäus
Dietz, Andreas
Wald, Theresa
Wichmann, Gunnar
author_sort Loermann, Gera
collection PubMed
description Detection of p16 through immunohistochemistry (IHC) is the standard for determining the HPV status of the tumor according the TNM eighth edition released in 2017 and has become crucial for determining the HPV status of oropharyngeal squamous cell carcinomas (OPSCC) with direct impact on staging and prognostication. In recent years, detection of HPV DNA in mouthwashes has been proposed as a noninvasive alternative, both for OPSCCs and for other head and neck squamous cell carcinomas (HNSCCs). However, the prospect of using the mouthwashes to monitor the response to therapy is unclear. To evaluate the effect of curative therapy on the detection of HPV DNA, we performed a prospective study comparing the detection frequency of high-risk HPV DNA (HR-HPV-DNA) in pre- and post-therapy mouthwashes. We collected 137 mouthwashes from 88 pathologically confirmed HNSCC patients for DNA isolation and HPV genotyping with the Inno-LiPA assay. We show that HPV DNA in pretherapeutic mouthwashes can detect HPV-driven HNSCCs with a sensitivity of 50.0% and specificity of 85.4%, alongside a high negative predictive value of 79.5% and an accuracy of 74.5%. Furthermore, we observed a notable decrease in the detection frequency of HR-HPV-DNA after successful treatment (pre-therapy 50.0% (9/18) versus post-therapy 9.7% (3/28)). However, the comparatively low sensitivity regarding detection of HPV-driven OPSCC argues against its use in clinical routine.
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spelling pubmed-95706612022-10-17 High-Risk Human Papillomavirus (HR-HPV) DNA Detection in Mouthwashes for Diagnosis of HPV-Driven Oropharynx Cancer and Its Curative Therapy—A Feasibility Study Loermann, Gera Kolb, Marlen Prascevic, Dusan Siemert, Julia Wiegand, Susanne Zebralla, Veit Pirlich, Markus Stöhr, Matthäus Dietz, Andreas Wald, Theresa Wichmann, Gunnar J Clin Med Article Detection of p16 through immunohistochemistry (IHC) is the standard for determining the HPV status of the tumor according the TNM eighth edition released in 2017 and has become crucial for determining the HPV status of oropharyngeal squamous cell carcinomas (OPSCC) with direct impact on staging and prognostication. In recent years, detection of HPV DNA in mouthwashes has been proposed as a noninvasive alternative, both for OPSCCs and for other head and neck squamous cell carcinomas (HNSCCs). However, the prospect of using the mouthwashes to monitor the response to therapy is unclear. To evaluate the effect of curative therapy on the detection of HPV DNA, we performed a prospective study comparing the detection frequency of high-risk HPV DNA (HR-HPV-DNA) in pre- and post-therapy mouthwashes. We collected 137 mouthwashes from 88 pathologically confirmed HNSCC patients for DNA isolation and HPV genotyping with the Inno-LiPA assay. We show that HPV DNA in pretherapeutic mouthwashes can detect HPV-driven HNSCCs with a sensitivity of 50.0% and specificity of 85.4%, alongside a high negative predictive value of 79.5% and an accuracy of 74.5%. Furthermore, we observed a notable decrease in the detection frequency of HR-HPV-DNA after successful treatment (pre-therapy 50.0% (9/18) versus post-therapy 9.7% (3/28)). However, the comparatively low sensitivity regarding detection of HPV-driven OPSCC argues against its use in clinical routine. MDPI 2022-09-20 /pmc/articles/PMC9570661/ /pubmed/36233378 http://dx.doi.org/10.3390/jcm11195509 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Loermann, Gera
Kolb, Marlen
Prascevic, Dusan
Siemert, Julia
Wiegand, Susanne
Zebralla, Veit
Pirlich, Markus
Stöhr, Matthäus
Dietz, Andreas
Wald, Theresa
Wichmann, Gunnar
High-Risk Human Papillomavirus (HR-HPV) DNA Detection in Mouthwashes for Diagnosis of HPV-Driven Oropharynx Cancer and Its Curative Therapy—A Feasibility Study
title High-Risk Human Papillomavirus (HR-HPV) DNA Detection in Mouthwashes for Diagnosis of HPV-Driven Oropharynx Cancer and Its Curative Therapy—A Feasibility Study
title_full High-Risk Human Papillomavirus (HR-HPV) DNA Detection in Mouthwashes for Diagnosis of HPV-Driven Oropharynx Cancer and Its Curative Therapy—A Feasibility Study
title_fullStr High-Risk Human Papillomavirus (HR-HPV) DNA Detection in Mouthwashes for Diagnosis of HPV-Driven Oropharynx Cancer and Its Curative Therapy—A Feasibility Study
title_full_unstemmed High-Risk Human Papillomavirus (HR-HPV) DNA Detection in Mouthwashes for Diagnosis of HPV-Driven Oropharynx Cancer and Its Curative Therapy—A Feasibility Study
title_short High-Risk Human Papillomavirus (HR-HPV) DNA Detection in Mouthwashes for Diagnosis of HPV-Driven Oropharynx Cancer and Its Curative Therapy—A Feasibility Study
title_sort high-risk human papillomavirus (hr-hpv) dna detection in mouthwashes for diagnosis of hpv-driven oropharynx cancer and its curative therapy—a feasibility study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9570661/
https://www.ncbi.nlm.nih.gov/pubmed/36233378
http://dx.doi.org/10.3390/jcm11195509
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