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Utilization of a Novel Immunofluorescence Instrument Prototype for the Determination of the Herbicide Glyphosate

An enzyme-linked fluorescent immunoassay (ELFIA) method has been developed for the quantitative analytical determination of the herbicide active ingredient glyphosate in environmental matrices (surface water, soil, and plant tissues). Glyphosate, as a ubiquitous agricultural pollutant, is a xenobiot...

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Autores principales: Takács, Eszter, Gémes, Borbála, Szendrei, Fanni, Keszei, Csaba, Barócsi, Attila, Lenk, Sándor, Domján, László, Mörtl, Mária, Székács, András
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9570942/
https://www.ncbi.nlm.nih.gov/pubmed/36235051
http://dx.doi.org/10.3390/molecules27196514
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author Takács, Eszter
Gémes, Borbála
Szendrei, Fanni
Keszei, Csaba
Barócsi, Attila
Lenk, Sándor
Domján, László
Mörtl, Mária
Székács, András
author_facet Takács, Eszter
Gémes, Borbála
Szendrei, Fanni
Keszei, Csaba
Barócsi, Attila
Lenk, Sándor
Domján, László
Mörtl, Mária
Székács, András
author_sort Takács, Eszter
collection PubMed
description An enzyme-linked fluorescent immunoassay (ELFIA) method has been developed for the quantitative analytical determination of the herbicide active ingredient glyphosate in environmental matrices (surface water, soil, and plant tissues). Glyphosate, as a ubiquitous agricultural pollutant, is a xenobiotic substance with exposure in aquatic and terrestrial ecosystems due its extremely high worldwide application rate. The immunoassay developed in Project Aquafluosense is part of a fluorescence-based instrumentation setup for the in situ determination of several characteristic water quality parameters. The 96-well microplate-based competitive immunoassay method applies fluorescence signal detection in the concentration range of 0–100 ng/mL glyphosate. Application of the fluorescent signal provides a limit of detection of 0.09 ng/mL, which is 2.5-fold lower than that obtained with a visual absorbance signal. Beside the improved limit of detection, determination by fluorescence provided a wider and steeper dynamic range for glyphosate detection. No matrix effect appeared for the undiluted surface water samples, while plant tissues and soil samples required dilution rates of 1:10 and 1:100, respectively. No cross-reaction was determined with the main metabolite of glyphosate, N-aminomethylphosphonic acid, and related compounds.
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spelling pubmed-95709422022-10-17 Utilization of a Novel Immunofluorescence Instrument Prototype for the Determination of the Herbicide Glyphosate Takács, Eszter Gémes, Borbála Szendrei, Fanni Keszei, Csaba Barócsi, Attila Lenk, Sándor Domján, László Mörtl, Mária Székács, András Molecules Article An enzyme-linked fluorescent immunoassay (ELFIA) method has been developed for the quantitative analytical determination of the herbicide active ingredient glyphosate in environmental matrices (surface water, soil, and plant tissues). Glyphosate, as a ubiquitous agricultural pollutant, is a xenobiotic substance with exposure in aquatic and terrestrial ecosystems due its extremely high worldwide application rate. The immunoassay developed in Project Aquafluosense is part of a fluorescence-based instrumentation setup for the in situ determination of several characteristic water quality parameters. The 96-well microplate-based competitive immunoassay method applies fluorescence signal detection in the concentration range of 0–100 ng/mL glyphosate. Application of the fluorescent signal provides a limit of detection of 0.09 ng/mL, which is 2.5-fold lower than that obtained with a visual absorbance signal. Beside the improved limit of detection, determination by fluorescence provided a wider and steeper dynamic range for glyphosate detection. No matrix effect appeared for the undiluted surface water samples, while plant tissues and soil samples required dilution rates of 1:10 and 1:100, respectively. No cross-reaction was determined with the main metabolite of glyphosate, N-aminomethylphosphonic acid, and related compounds. MDPI 2022-10-02 /pmc/articles/PMC9570942/ /pubmed/36235051 http://dx.doi.org/10.3390/molecules27196514 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Takács, Eszter
Gémes, Borbála
Szendrei, Fanni
Keszei, Csaba
Barócsi, Attila
Lenk, Sándor
Domján, László
Mörtl, Mária
Székács, András
Utilization of a Novel Immunofluorescence Instrument Prototype for the Determination of the Herbicide Glyphosate
title Utilization of a Novel Immunofluorescence Instrument Prototype for the Determination of the Herbicide Glyphosate
title_full Utilization of a Novel Immunofluorescence Instrument Prototype for the Determination of the Herbicide Glyphosate
title_fullStr Utilization of a Novel Immunofluorescence Instrument Prototype for the Determination of the Herbicide Glyphosate
title_full_unstemmed Utilization of a Novel Immunofluorescence Instrument Prototype for the Determination of the Herbicide Glyphosate
title_short Utilization of a Novel Immunofluorescence Instrument Prototype for the Determination of the Herbicide Glyphosate
title_sort utilization of a novel immunofluorescence instrument prototype for the determination of the herbicide glyphosate
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9570942/
https://www.ncbi.nlm.nih.gov/pubmed/36235051
http://dx.doi.org/10.3390/molecules27196514
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