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Utilization of a Novel Immunofluorescence Instrument Prototype for the Determination of the Herbicide Glyphosate
An enzyme-linked fluorescent immunoassay (ELFIA) method has been developed for the quantitative analytical determination of the herbicide active ingredient glyphosate in environmental matrices (surface water, soil, and plant tissues). Glyphosate, as a ubiquitous agricultural pollutant, is a xenobiot...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9570942/ https://www.ncbi.nlm.nih.gov/pubmed/36235051 http://dx.doi.org/10.3390/molecules27196514 |
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author | Takács, Eszter Gémes, Borbála Szendrei, Fanni Keszei, Csaba Barócsi, Attila Lenk, Sándor Domján, László Mörtl, Mária Székács, András |
author_facet | Takács, Eszter Gémes, Borbála Szendrei, Fanni Keszei, Csaba Barócsi, Attila Lenk, Sándor Domján, László Mörtl, Mária Székács, András |
author_sort | Takács, Eszter |
collection | PubMed |
description | An enzyme-linked fluorescent immunoassay (ELFIA) method has been developed for the quantitative analytical determination of the herbicide active ingredient glyphosate in environmental matrices (surface water, soil, and plant tissues). Glyphosate, as a ubiquitous agricultural pollutant, is a xenobiotic substance with exposure in aquatic and terrestrial ecosystems due its extremely high worldwide application rate. The immunoassay developed in Project Aquafluosense is part of a fluorescence-based instrumentation setup for the in situ determination of several characteristic water quality parameters. The 96-well microplate-based competitive immunoassay method applies fluorescence signal detection in the concentration range of 0–100 ng/mL glyphosate. Application of the fluorescent signal provides a limit of detection of 0.09 ng/mL, which is 2.5-fold lower than that obtained with a visual absorbance signal. Beside the improved limit of detection, determination by fluorescence provided a wider and steeper dynamic range for glyphosate detection. No matrix effect appeared for the undiluted surface water samples, while plant tissues and soil samples required dilution rates of 1:10 and 1:100, respectively. No cross-reaction was determined with the main metabolite of glyphosate, N-aminomethylphosphonic acid, and related compounds. |
format | Online Article Text |
id | pubmed-9570942 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-95709422022-10-17 Utilization of a Novel Immunofluorescence Instrument Prototype for the Determination of the Herbicide Glyphosate Takács, Eszter Gémes, Borbála Szendrei, Fanni Keszei, Csaba Barócsi, Attila Lenk, Sándor Domján, László Mörtl, Mária Székács, András Molecules Article An enzyme-linked fluorescent immunoassay (ELFIA) method has been developed for the quantitative analytical determination of the herbicide active ingredient glyphosate in environmental matrices (surface water, soil, and plant tissues). Glyphosate, as a ubiquitous agricultural pollutant, is a xenobiotic substance with exposure in aquatic and terrestrial ecosystems due its extremely high worldwide application rate. The immunoassay developed in Project Aquafluosense is part of a fluorescence-based instrumentation setup for the in situ determination of several characteristic water quality parameters. The 96-well microplate-based competitive immunoassay method applies fluorescence signal detection in the concentration range of 0–100 ng/mL glyphosate. Application of the fluorescent signal provides a limit of detection of 0.09 ng/mL, which is 2.5-fold lower than that obtained with a visual absorbance signal. Beside the improved limit of detection, determination by fluorescence provided a wider and steeper dynamic range for glyphosate detection. No matrix effect appeared for the undiluted surface water samples, while plant tissues and soil samples required dilution rates of 1:10 and 1:100, respectively. No cross-reaction was determined with the main metabolite of glyphosate, N-aminomethylphosphonic acid, and related compounds. MDPI 2022-10-02 /pmc/articles/PMC9570942/ /pubmed/36235051 http://dx.doi.org/10.3390/molecules27196514 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Takács, Eszter Gémes, Borbála Szendrei, Fanni Keszei, Csaba Barócsi, Attila Lenk, Sándor Domján, László Mörtl, Mária Székács, András Utilization of a Novel Immunofluorescence Instrument Prototype for the Determination of the Herbicide Glyphosate |
title | Utilization of a Novel Immunofluorescence Instrument Prototype for the Determination of the Herbicide Glyphosate |
title_full | Utilization of a Novel Immunofluorescence Instrument Prototype for the Determination of the Herbicide Glyphosate |
title_fullStr | Utilization of a Novel Immunofluorescence Instrument Prototype for the Determination of the Herbicide Glyphosate |
title_full_unstemmed | Utilization of a Novel Immunofluorescence Instrument Prototype for the Determination of the Herbicide Glyphosate |
title_short | Utilization of a Novel Immunofluorescence Instrument Prototype for the Determination of the Herbicide Glyphosate |
title_sort | utilization of a novel immunofluorescence instrument prototype for the determination of the herbicide glyphosate |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9570942/ https://www.ncbi.nlm.nih.gov/pubmed/36235051 http://dx.doi.org/10.3390/molecules27196514 |
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