Cargando…

Chain-End Functionalization of Poly(ε-caprolactone) for Chemical Binding with Gelatin: Binary Electrospun Scaffolds with Improved Physico-Mechanical Characteristics and Cell Adhesive Properties

Composite biocompatible scaffolds, obtained using the electrospinning (ES) technique, are highly promising for biomedical application thanks to their high surface area, porosity, adjustable fiber diameter, and permeability. However, the combination of synthetic biodegradable (such as poly(ε-caprolac...

Descripción completa

Detalles Bibliográficos
Autores principales: Nifant’ev, Ilya, Besprozvannykh, Victoria, Shlyakhtin, Andrey, Tavtorkin, Alexander, Legkov, Sergei, Chinova, Maria, Arutyunyan, Irina, Soboleva, Anna, Fatkhudinov, Timur, Ivchenko, Pavel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9570970/
https://www.ncbi.nlm.nih.gov/pubmed/36236153
http://dx.doi.org/10.3390/polym14194203
_version_ 1784810245444337664
author Nifant’ev, Ilya
Besprozvannykh, Victoria
Shlyakhtin, Andrey
Tavtorkin, Alexander
Legkov, Sergei
Chinova, Maria
Arutyunyan, Irina
Soboleva, Anna
Fatkhudinov, Timur
Ivchenko, Pavel
author_facet Nifant’ev, Ilya
Besprozvannykh, Victoria
Shlyakhtin, Andrey
Tavtorkin, Alexander
Legkov, Sergei
Chinova, Maria
Arutyunyan, Irina
Soboleva, Anna
Fatkhudinov, Timur
Ivchenko, Pavel
author_sort Nifant’ev, Ilya
collection PubMed
description Composite biocompatible scaffolds, obtained using the electrospinning (ES) technique, are highly promising for biomedical application thanks to their high surface area, porosity, adjustable fiber diameter, and permeability. However, the combination of synthetic biodegradable (such as poly(ε-caprolactone) PCL) and natural (such as gelatin Gt) polymers is complicated by the problem of low compatibility of the components. Previously, this problem was solved by PCL grafting and/or Gt cross-linking after ES molding. In the present study, composite fibrous scaffolds consisting of PCL and Gt were fabricated by the electrospinning (ES) method using non-functionalized PCL1 or NHS-functionalized PCL2 and hexafluoroisopropanol as a solvent. To provide covalent binding between PCL2 and Gt macromolecules, NHS-functionalized methyl glutarate was synthesized and studied in model reactions with components of spinning solution. It was found that selective formation of amide bonds, which provide complete covalent bonding of Gt in PCL/Gt composite, requires the presence of weak acid. With the use of the optimized ES method, fibrous mats with different PCL/Gt ratios were prepared. The sample morphology (SEM), hydrolytic resistance (FT-IR), cell adhesion and viability (MTT assay), cell penetration (fluorescent microscopy), and mechanical characteristics of the samples were studied. PCL2-based films with a Gt content of 20 wt% have demonstrated the best set of properties.
format Online
Article
Text
id pubmed-9570970
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-95709702022-10-17 Chain-End Functionalization of Poly(ε-caprolactone) for Chemical Binding with Gelatin: Binary Electrospun Scaffolds with Improved Physico-Mechanical Characteristics and Cell Adhesive Properties Nifant’ev, Ilya Besprozvannykh, Victoria Shlyakhtin, Andrey Tavtorkin, Alexander Legkov, Sergei Chinova, Maria Arutyunyan, Irina Soboleva, Anna Fatkhudinov, Timur Ivchenko, Pavel Polymers (Basel) Article Composite biocompatible scaffolds, obtained using the electrospinning (ES) technique, are highly promising for biomedical application thanks to their high surface area, porosity, adjustable fiber diameter, and permeability. However, the combination of synthetic biodegradable (such as poly(ε-caprolactone) PCL) and natural (such as gelatin Gt) polymers is complicated by the problem of low compatibility of the components. Previously, this problem was solved by PCL grafting and/or Gt cross-linking after ES molding. In the present study, composite fibrous scaffolds consisting of PCL and Gt were fabricated by the electrospinning (ES) method using non-functionalized PCL1 or NHS-functionalized PCL2 and hexafluoroisopropanol as a solvent. To provide covalent binding between PCL2 and Gt macromolecules, NHS-functionalized methyl glutarate was synthesized and studied in model reactions with components of spinning solution. It was found that selective formation of amide bonds, which provide complete covalent bonding of Gt in PCL/Gt composite, requires the presence of weak acid. With the use of the optimized ES method, fibrous mats with different PCL/Gt ratios were prepared. The sample morphology (SEM), hydrolytic resistance (FT-IR), cell adhesion and viability (MTT assay), cell penetration (fluorescent microscopy), and mechanical characteristics of the samples were studied. PCL2-based films with a Gt content of 20 wt% have demonstrated the best set of properties. MDPI 2022-10-07 /pmc/articles/PMC9570970/ /pubmed/36236153 http://dx.doi.org/10.3390/polym14194203 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nifant’ev, Ilya
Besprozvannykh, Victoria
Shlyakhtin, Andrey
Tavtorkin, Alexander
Legkov, Sergei
Chinova, Maria
Arutyunyan, Irina
Soboleva, Anna
Fatkhudinov, Timur
Ivchenko, Pavel
Chain-End Functionalization of Poly(ε-caprolactone) for Chemical Binding with Gelatin: Binary Electrospun Scaffolds with Improved Physico-Mechanical Characteristics and Cell Adhesive Properties
title Chain-End Functionalization of Poly(ε-caprolactone) for Chemical Binding with Gelatin: Binary Electrospun Scaffolds with Improved Physico-Mechanical Characteristics and Cell Adhesive Properties
title_full Chain-End Functionalization of Poly(ε-caprolactone) for Chemical Binding with Gelatin: Binary Electrospun Scaffolds with Improved Physico-Mechanical Characteristics and Cell Adhesive Properties
title_fullStr Chain-End Functionalization of Poly(ε-caprolactone) for Chemical Binding with Gelatin: Binary Electrospun Scaffolds with Improved Physico-Mechanical Characteristics and Cell Adhesive Properties
title_full_unstemmed Chain-End Functionalization of Poly(ε-caprolactone) for Chemical Binding with Gelatin: Binary Electrospun Scaffolds with Improved Physico-Mechanical Characteristics and Cell Adhesive Properties
title_short Chain-End Functionalization of Poly(ε-caprolactone) for Chemical Binding with Gelatin: Binary Electrospun Scaffolds with Improved Physico-Mechanical Characteristics and Cell Adhesive Properties
title_sort chain-end functionalization of poly(ε-caprolactone) for chemical binding with gelatin: binary electrospun scaffolds with improved physico-mechanical characteristics and cell adhesive properties
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9570970/
https://www.ncbi.nlm.nih.gov/pubmed/36236153
http://dx.doi.org/10.3390/polym14194203
work_keys_str_mv AT nifantevilya chainendfunctionalizationofpolyecaprolactoneforchemicalbindingwithgelatinbinaryelectrospunscaffoldswithimprovedphysicomechanicalcharacteristicsandcelladhesiveproperties
AT besprozvannykhvictoria chainendfunctionalizationofpolyecaprolactoneforchemicalbindingwithgelatinbinaryelectrospunscaffoldswithimprovedphysicomechanicalcharacteristicsandcelladhesiveproperties
AT shlyakhtinandrey chainendfunctionalizationofpolyecaprolactoneforchemicalbindingwithgelatinbinaryelectrospunscaffoldswithimprovedphysicomechanicalcharacteristicsandcelladhesiveproperties
AT tavtorkinalexander chainendfunctionalizationofpolyecaprolactoneforchemicalbindingwithgelatinbinaryelectrospunscaffoldswithimprovedphysicomechanicalcharacteristicsandcelladhesiveproperties
AT legkovsergei chainendfunctionalizationofpolyecaprolactoneforchemicalbindingwithgelatinbinaryelectrospunscaffoldswithimprovedphysicomechanicalcharacteristicsandcelladhesiveproperties
AT chinovamaria chainendfunctionalizationofpolyecaprolactoneforchemicalbindingwithgelatinbinaryelectrospunscaffoldswithimprovedphysicomechanicalcharacteristicsandcelladhesiveproperties
AT arutyunyanirina chainendfunctionalizationofpolyecaprolactoneforchemicalbindingwithgelatinbinaryelectrospunscaffoldswithimprovedphysicomechanicalcharacteristicsandcelladhesiveproperties
AT sobolevaanna chainendfunctionalizationofpolyecaprolactoneforchemicalbindingwithgelatinbinaryelectrospunscaffoldswithimprovedphysicomechanicalcharacteristicsandcelladhesiveproperties
AT fatkhudinovtimur chainendfunctionalizationofpolyecaprolactoneforchemicalbindingwithgelatinbinaryelectrospunscaffoldswithimprovedphysicomechanicalcharacteristicsandcelladhesiveproperties
AT ivchenkopavel chainendfunctionalizationofpolyecaprolactoneforchemicalbindingwithgelatinbinaryelectrospunscaffoldswithimprovedphysicomechanicalcharacteristicsandcelladhesiveproperties