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Three-Fluorophore FRET Enables the Analysis of Ternary Protein Association in Living Plant Cells

Protein-protein interaction studies provide valuable insights into cellular signaling. Brassinosteroid (BR) signaling is initiated by the hormone-binding receptor Brassinosteroid Insensitive 1 (BRI1) and its co-receptor BRI1 Associated Kinase 1 (BAK1). BRI1 and BAK1 were shown to interact independen...

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Autores principales: Glöckner, Nina, zur Oven-Krockhaus, Sven, Rohr, Leander, Wackenhut, Frank, Burmeister, Moritz, Wanke, Friederike, Holzwart, Eleonore, Meixner, Alfred J., Wolf, Sebastian, Harter, Klaus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9571070/
https://www.ncbi.nlm.nih.gov/pubmed/36235497
http://dx.doi.org/10.3390/plants11192630
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author Glöckner, Nina
zur Oven-Krockhaus, Sven
Rohr, Leander
Wackenhut, Frank
Burmeister, Moritz
Wanke, Friederike
Holzwart, Eleonore
Meixner, Alfred J.
Wolf, Sebastian
Harter, Klaus
author_facet Glöckner, Nina
zur Oven-Krockhaus, Sven
Rohr, Leander
Wackenhut, Frank
Burmeister, Moritz
Wanke, Friederike
Holzwart, Eleonore
Meixner, Alfred J.
Wolf, Sebastian
Harter, Klaus
author_sort Glöckner, Nina
collection PubMed
description Protein-protein interaction studies provide valuable insights into cellular signaling. Brassinosteroid (BR) signaling is initiated by the hormone-binding receptor Brassinosteroid Insensitive 1 (BRI1) and its co-receptor BRI1 Associated Kinase 1 (BAK1). BRI1 and BAK1 were shown to interact independently with the Receptor-Like Protein 44 (RLP44), which is implicated in BRI1/BAK1-dependent cell wall integrity perception. To demonstrate the proposed complex formation of BRI1, BAK1 and RLP44, we established three-fluorophore intensity-based spectral Förster resonance energy transfer (FRET) and FRET-fluorescence lifetime imaging microscopy (FLIM) for living plant cells. Our evidence indicates that RLP44, BRI1 and BAK1 form a ternary complex in a distinct plasma membrane nanodomain. In contrast, although the immune receptor Flagellin Sensing 2 (FLS2) also forms a heteromer with BAK1, the FLS2/BAK1 complexes are localized to other nanodomains. In conclusion, both three-fluorophore FRET approaches provide a feasible basis for studying the in vivo interaction and sub-compartmentalization of proteins in great detail.
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spelling pubmed-95710702022-10-17 Three-Fluorophore FRET Enables the Analysis of Ternary Protein Association in Living Plant Cells Glöckner, Nina zur Oven-Krockhaus, Sven Rohr, Leander Wackenhut, Frank Burmeister, Moritz Wanke, Friederike Holzwart, Eleonore Meixner, Alfred J. Wolf, Sebastian Harter, Klaus Plants (Basel) Article Protein-protein interaction studies provide valuable insights into cellular signaling. Brassinosteroid (BR) signaling is initiated by the hormone-binding receptor Brassinosteroid Insensitive 1 (BRI1) and its co-receptor BRI1 Associated Kinase 1 (BAK1). BRI1 and BAK1 were shown to interact independently with the Receptor-Like Protein 44 (RLP44), which is implicated in BRI1/BAK1-dependent cell wall integrity perception. To demonstrate the proposed complex formation of BRI1, BAK1 and RLP44, we established three-fluorophore intensity-based spectral Förster resonance energy transfer (FRET) and FRET-fluorescence lifetime imaging microscopy (FLIM) for living plant cells. Our evidence indicates that RLP44, BRI1 and BAK1 form a ternary complex in a distinct plasma membrane nanodomain. In contrast, although the immune receptor Flagellin Sensing 2 (FLS2) also forms a heteromer with BAK1, the FLS2/BAK1 complexes are localized to other nanodomains. In conclusion, both three-fluorophore FRET approaches provide a feasible basis for studying the in vivo interaction and sub-compartmentalization of proteins in great detail. MDPI 2022-10-06 /pmc/articles/PMC9571070/ /pubmed/36235497 http://dx.doi.org/10.3390/plants11192630 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Glöckner, Nina
zur Oven-Krockhaus, Sven
Rohr, Leander
Wackenhut, Frank
Burmeister, Moritz
Wanke, Friederike
Holzwart, Eleonore
Meixner, Alfred J.
Wolf, Sebastian
Harter, Klaus
Three-Fluorophore FRET Enables the Analysis of Ternary Protein Association in Living Plant Cells
title Three-Fluorophore FRET Enables the Analysis of Ternary Protein Association in Living Plant Cells
title_full Three-Fluorophore FRET Enables the Analysis of Ternary Protein Association in Living Plant Cells
title_fullStr Three-Fluorophore FRET Enables the Analysis of Ternary Protein Association in Living Plant Cells
title_full_unstemmed Three-Fluorophore FRET Enables the Analysis of Ternary Protein Association in Living Plant Cells
title_short Three-Fluorophore FRET Enables the Analysis of Ternary Protein Association in Living Plant Cells
title_sort three-fluorophore fret enables the analysis of ternary protein association in living plant cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9571070/
https://www.ncbi.nlm.nih.gov/pubmed/36235497
http://dx.doi.org/10.3390/plants11192630
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