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Interaction of the Fungal Metabolite Harzianic Acid with Rare-Earth Cations (Pr(3+), Eu(3+), Ho(3+), Tm(3+))

Rare-earth elements (REEs) are in all respect a class of new contaminants that may have toxic effects on organisms and microorganisms and information on their interactions with natural ligands should be of value to predict and control their diffusion in natural environments. In the current study, we...

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Autores principales: Salvatore, Maria Michela, Siciliano, Antonietta, Staropoli, Alessia, Vinale, Francesco, Nicoletti, Rosario, DellaGreca, Marina, Guida, Marco, Salvatore, Francesco, Iuliano, Mauro, Andolfi, Anna, De Tommaso, Gaetano
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9571137/
https://www.ncbi.nlm.nih.gov/pubmed/36235005
http://dx.doi.org/10.3390/molecules27196468
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author Salvatore, Maria Michela
Siciliano, Antonietta
Staropoli, Alessia
Vinale, Francesco
Nicoletti, Rosario
DellaGreca, Marina
Guida, Marco
Salvatore, Francesco
Iuliano, Mauro
Andolfi, Anna
De Tommaso, Gaetano
author_facet Salvatore, Maria Michela
Siciliano, Antonietta
Staropoli, Alessia
Vinale, Francesco
Nicoletti, Rosario
DellaGreca, Marina
Guida, Marco
Salvatore, Francesco
Iuliano, Mauro
Andolfi, Anna
De Tommaso, Gaetano
author_sort Salvatore, Maria Michela
collection PubMed
description Rare-earth elements (REEs) are in all respect a class of new contaminants that may have toxic effects on organisms and microorganisms and information on their interactions with natural ligands should be of value to predict and control their diffusion in natural environments. In the current study, we investigate interactions of tripositive cations of praseodymium, europium, holmium, and thulium with harzianic acid (H(2)L), a secondary metabolite produced by selected strains of fungi belonging to the Trichoderma genus. We applied the same techniques and workflow previously employed in an analogous study concerning lanthanum, neodymium, samarium, and gadolinium tripositive cations. Therefore, in the current study, HPLC-ESI-HRMS experiments, circular dichroism (CD), and UV-Vis spectrophotometric absorption data, as well as accurate pH measurements, were applied to characterize bonding interactions between harzianic acid and Pr(3+), Eu(3+), Ho(3+), and Tm(3+) cations. Problems connected to the low solubility of harzianic acid in water were overcome by employing a 0.1 M NaClO(4)/(CH(3)OH + H(2)O 50/50 w/w) mixed solvent. For Pr(3+), Ho(3+), and Tm(3+), only the mono complexes PrL(+), HoL(+), and TmL(+) were detected and their formation constant determined. Eu(3+) forms almost exclusively the bis complex [Formula: see text] for which the corresponding formation constant is reported; under our experimental conditions, the mono complex EuL(+) is irrelevant. Combining the results of the present and previous studies, a picture of interactions of harzianic acid with rare-earth cations extending over 8 of the 17 REEs can be composed. In order to complement chemical information with toxicological information, a battery of bioassays was applied to evaluate the effects of praseodymium, europium, holmium, and thulium tripositive cations on a suite of bioindicators including Aliivibrio fischeri (Gram-negative bacterium), Raphidocelis subcapitata (green alga), and Daphnia magna (microcrustacean), and median effective concentration (EC50) values of Pr(3+), Eu(3+), Ho(3+), and Tm(3+) for the tested species were assessed.
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spelling pubmed-95711372022-10-17 Interaction of the Fungal Metabolite Harzianic Acid with Rare-Earth Cations (Pr(3+), Eu(3+), Ho(3+), Tm(3+)) Salvatore, Maria Michela Siciliano, Antonietta Staropoli, Alessia Vinale, Francesco Nicoletti, Rosario DellaGreca, Marina Guida, Marco Salvatore, Francesco Iuliano, Mauro Andolfi, Anna De Tommaso, Gaetano Molecules Article Rare-earth elements (REEs) are in all respect a class of new contaminants that may have toxic effects on organisms and microorganisms and information on their interactions with natural ligands should be of value to predict and control their diffusion in natural environments. In the current study, we investigate interactions of tripositive cations of praseodymium, europium, holmium, and thulium with harzianic acid (H(2)L), a secondary metabolite produced by selected strains of fungi belonging to the Trichoderma genus. We applied the same techniques and workflow previously employed in an analogous study concerning lanthanum, neodymium, samarium, and gadolinium tripositive cations. Therefore, in the current study, HPLC-ESI-HRMS experiments, circular dichroism (CD), and UV-Vis spectrophotometric absorption data, as well as accurate pH measurements, were applied to characterize bonding interactions between harzianic acid and Pr(3+), Eu(3+), Ho(3+), and Tm(3+) cations. Problems connected to the low solubility of harzianic acid in water were overcome by employing a 0.1 M NaClO(4)/(CH(3)OH + H(2)O 50/50 w/w) mixed solvent. For Pr(3+), Ho(3+), and Tm(3+), only the mono complexes PrL(+), HoL(+), and TmL(+) were detected and their formation constant determined. Eu(3+) forms almost exclusively the bis complex [Formula: see text] for which the corresponding formation constant is reported; under our experimental conditions, the mono complex EuL(+) is irrelevant. Combining the results of the present and previous studies, a picture of interactions of harzianic acid with rare-earth cations extending over 8 of the 17 REEs can be composed. In order to complement chemical information with toxicological information, a battery of bioassays was applied to evaluate the effects of praseodymium, europium, holmium, and thulium tripositive cations on a suite of bioindicators including Aliivibrio fischeri (Gram-negative bacterium), Raphidocelis subcapitata (green alga), and Daphnia magna (microcrustacean), and median effective concentration (EC50) values of Pr(3+), Eu(3+), Ho(3+), and Tm(3+) for the tested species were assessed. MDPI 2022-10-01 /pmc/articles/PMC9571137/ /pubmed/36235005 http://dx.doi.org/10.3390/molecules27196468 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Salvatore, Maria Michela
Siciliano, Antonietta
Staropoli, Alessia
Vinale, Francesco
Nicoletti, Rosario
DellaGreca, Marina
Guida, Marco
Salvatore, Francesco
Iuliano, Mauro
Andolfi, Anna
De Tommaso, Gaetano
Interaction of the Fungal Metabolite Harzianic Acid with Rare-Earth Cations (Pr(3+), Eu(3+), Ho(3+), Tm(3+))
title Interaction of the Fungal Metabolite Harzianic Acid with Rare-Earth Cations (Pr(3+), Eu(3+), Ho(3+), Tm(3+))
title_full Interaction of the Fungal Metabolite Harzianic Acid with Rare-Earth Cations (Pr(3+), Eu(3+), Ho(3+), Tm(3+))
title_fullStr Interaction of the Fungal Metabolite Harzianic Acid with Rare-Earth Cations (Pr(3+), Eu(3+), Ho(3+), Tm(3+))
title_full_unstemmed Interaction of the Fungal Metabolite Harzianic Acid with Rare-Earth Cations (Pr(3+), Eu(3+), Ho(3+), Tm(3+))
title_short Interaction of the Fungal Metabolite Harzianic Acid with Rare-Earth Cations (Pr(3+), Eu(3+), Ho(3+), Tm(3+))
title_sort interaction of the fungal metabolite harzianic acid with rare-earth cations (pr(3+), eu(3+), ho(3+), tm(3+))
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9571137/
https://www.ncbi.nlm.nih.gov/pubmed/36235005
http://dx.doi.org/10.3390/molecules27196468
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