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Mapping of Two Major QTLs Controlling Flowering Time in Brassica napus Using a High-Density Genetic Map

Research on the flowering habit of rapeseed is important for the selection of varieties adapted to specific ecological environments. Here, quantitative trait loci (QTL) for the days-to-flowering trait were identified using a doubled haploid population of 178 lines derived from a cross between the wi...

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Autores principales: Chen, Lei, Lei, Weixia, He, Wangfei, Wang, Yifan, Tian, Jie, Gong, Jihui, Hao, Bing, Cheng, Xinxin, Shu, Yingjie, Fan, Zhixiong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9571212/
https://www.ncbi.nlm.nih.gov/pubmed/36235500
http://dx.doi.org/10.3390/plants11192635
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author Chen, Lei
Lei, Weixia
He, Wangfei
Wang, Yifan
Tian, Jie
Gong, Jihui
Hao, Bing
Cheng, Xinxin
Shu, Yingjie
Fan, Zhixiong
author_facet Chen, Lei
Lei, Weixia
He, Wangfei
Wang, Yifan
Tian, Jie
Gong, Jihui
Hao, Bing
Cheng, Xinxin
Shu, Yingjie
Fan, Zhixiong
author_sort Chen, Lei
collection PubMed
description Research on the flowering habit of rapeseed is important for the selection of varieties adapted to specific ecological environments. Here, quantitative trait loci (QTL) for the days-to-flowering trait were identified using a doubled haploid population of 178 lines derived from a cross between the winter type SGDH284 and the semi-winter type 158A. A linkage map encompassing 3268.01 cM was constructed using 2777 bin markers obtained from next-generation sequencing. The preliminary mapping results revealed 56 QTLs for the days to flowering in the six replicates in the three environments. Twelve consensus QTLs were identified by a QTL meta-analysis, two of which (cqDTF-C02 and cqDTF-C06) were designated as major QTLs. Based on the micro-collinearity of the target regions between B. napus and Arabidopsis, four genes possibly related to flowering time were identified in the cqDTF-C02 interval, and only one gene possibly related to flowering time was identified in the cqDTF-C06 interval. A tightly linked insertion–deletion marker for the cqFT-C02 locus was developed. These findings will aid the breeding of early maturing B. napus varieties.
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spelling pubmed-95712122022-10-17 Mapping of Two Major QTLs Controlling Flowering Time in Brassica napus Using a High-Density Genetic Map Chen, Lei Lei, Weixia He, Wangfei Wang, Yifan Tian, Jie Gong, Jihui Hao, Bing Cheng, Xinxin Shu, Yingjie Fan, Zhixiong Plants (Basel) Article Research on the flowering habit of rapeseed is important for the selection of varieties adapted to specific ecological environments. Here, quantitative trait loci (QTL) for the days-to-flowering trait were identified using a doubled haploid population of 178 lines derived from a cross between the winter type SGDH284 and the semi-winter type 158A. A linkage map encompassing 3268.01 cM was constructed using 2777 bin markers obtained from next-generation sequencing. The preliminary mapping results revealed 56 QTLs for the days to flowering in the six replicates in the three environments. Twelve consensus QTLs were identified by a QTL meta-analysis, two of which (cqDTF-C02 and cqDTF-C06) were designated as major QTLs. Based on the micro-collinearity of the target regions between B. napus and Arabidopsis, four genes possibly related to flowering time were identified in the cqDTF-C02 interval, and only one gene possibly related to flowering time was identified in the cqDTF-C06 interval. A tightly linked insertion–deletion marker for the cqFT-C02 locus was developed. These findings will aid the breeding of early maturing B. napus varieties. MDPI 2022-10-07 /pmc/articles/PMC9571212/ /pubmed/36235500 http://dx.doi.org/10.3390/plants11192635 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chen, Lei
Lei, Weixia
He, Wangfei
Wang, Yifan
Tian, Jie
Gong, Jihui
Hao, Bing
Cheng, Xinxin
Shu, Yingjie
Fan, Zhixiong
Mapping of Two Major QTLs Controlling Flowering Time in Brassica napus Using a High-Density Genetic Map
title Mapping of Two Major QTLs Controlling Flowering Time in Brassica napus Using a High-Density Genetic Map
title_full Mapping of Two Major QTLs Controlling Flowering Time in Brassica napus Using a High-Density Genetic Map
title_fullStr Mapping of Two Major QTLs Controlling Flowering Time in Brassica napus Using a High-Density Genetic Map
title_full_unstemmed Mapping of Two Major QTLs Controlling Flowering Time in Brassica napus Using a High-Density Genetic Map
title_short Mapping of Two Major QTLs Controlling Flowering Time in Brassica napus Using a High-Density Genetic Map
title_sort mapping of two major qtls controlling flowering time in brassica napus using a high-density genetic map
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9571212/
https://www.ncbi.nlm.nih.gov/pubmed/36235500
http://dx.doi.org/10.3390/plants11192635
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