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Research of Water Molecules Cluster Structuring during Haberlea rhodopensis Friv. Hydration

Gesneriaceae plant family is comprised of resurrection species, namely Boea hygrometrica and Paraboea rufescens, that are native to the Southeast Asia and Haberlea rhodopensis, Ramonda myconi, and Ramonda serbica, which are mainly found in the Balkan Peninsula. Haberlea rhodopensis is known to be ab...

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Autores principales: Ignatov, Ignat, Huether, Fabio, Neshev, Nikolai, Kiselova-Kaneva, Yoana, Popova, Teodora P., Bankova, Ralitsa, Valcheva, Nedyalka, Ignatov, Alexander I., Angelcheva, Mariana, Angushev, Ivan, Baiti, Sadek
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9572004/
https://www.ncbi.nlm.nih.gov/pubmed/36235522
http://dx.doi.org/10.3390/plants11192655
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author Ignatov, Ignat
Huether, Fabio
Neshev, Nikolai
Kiselova-Kaneva, Yoana
Popova, Teodora P.
Bankova, Ralitsa
Valcheva, Nedyalka
Ignatov, Alexander I.
Angelcheva, Mariana
Angushev, Ivan
Baiti, Sadek
author_facet Ignatov, Ignat
Huether, Fabio
Neshev, Nikolai
Kiselova-Kaneva, Yoana
Popova, Teodora P.
Bankova, Ralitsa
Valcheva, Nedyalka
Ignatov, Alexander I.
Angelcheva, Mariana
Angushev, Ivan
Baiti, Sadek
author_sort Ignatov, Ignat
collection PubMed
description Gesneriaceae plant family is comprised of resurrection species, namely Boea hygrometrica and Paraboea rufescens, that are native to the Southeast Asia and Haberlea rhodopensis, Ramonda myconi, and Ramonda serbica, which are mainly found in the Balkan Peninsula. Haberlea rhodopensis is known to be able to survive extreme and prolonged dehydration. Study was carried out after the dried plant Haberlea rhodopensis Friv. had been hydrated and had reached its fresh state. Two juice samples were collected from the plant blossom: The first sample was prepared with 1% filtered water through a patented EVOdrop device. Then the sample was saturated with hydrogen with EVOdrop booster to a concentration of 1.2 ppm, pH = 7.3, ORP = −390 mV. This first sample was prepared with filtered tap water from Sofia, Bulgaria. The second sample, which was a control one, was developed with tap water from Sofia, Bulgaria, consisting of 1% solutions of Haberlea rhodopensis. A study revealed that during the drying process in H. rhodopensis the number of free water molecules decreases, and water dimers are formed. The aim of our study was to determine the number of water molecules in clusters in 1% solutions of hydrated H. rhodopensis plants. Results were analyzed according to the two types of water used in the experiment. Th EVOdrop device is equipped with an ultranano membrane and rotating jet nozzle to create a vortex water and saturation thanks to a second device EVObooster to obtain hydrogen-rich water. In the current study Hydrogen-rich water is referred to as Hydrogen EVOdrop Water (HEW). Research was conducted using the following methods—spectral methods non-equilibrium energy spectrum (NES) and differential non-equilibrium energy spectrum (DNES), mathematical models, and study of the distribution of water molecules in water clusters. In a licensed Eurotest Laboratory, the research of tap water before and after flowing through the EVOdrop device was proven. Studies have been carried out on the structuring of water molecule clusters after change of hydrogen bond energies. The restructuring comes with rearrangement of water molecules by the energy levels of hydrogen bonds. Local extrema can be observed in the spectrum with largest amount of water molecules. The structural changes were tested using the NES and DNES spectral methods. The conducted research proved that the application of EVOdrop device and EVObooster changes the parameters of water to benefit hydration and health.
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spelling pubmed-95720042022-10-17 Research of Water Molecules Cluster Structuring during Haberlea rhodopensis Friv. Hydration Ignatov, Ignat Huether, Fabio Neshev, Nikolai Kiselova-Kaneva, Yoana Popova, Teodora P. Bankova, Ralitsa Valcheva, Nedyalka Ignatov, Alexander I. Angelcheva, Mariana Angushev, Ivan Baiti, Sadek Plants (Basel) Article Gesneriaceae plant family is comprised of resurrection species, namely Boea hygrometrica and Paraboea rufescens, that are native to the Southeast Asia and Haberlea rhodopensis, Ramonda myconi, and Ramonda serbica, which are mainly found in the Balkan Peninsula. Haberlea rhodopensis is known to be able to survive extreme and prolonged dehydration. Study was carried out after the dried plant Haberlea rhodopensis Friv. had been hydrated and had reached its fresh state. Two juice samples were collected from the plant blossom: The first sample was prepared with 1% filtered water through a patented EVOdrop device. Then the sample was saturated with hydrogen with EVOdrop booster to a concentration of 1.2 ppm, pH = 7.3, ORP = −390 mV. This first sample was prepared with filtered tap water from Sofia, Bulgaria. The second sample, which was a control one, was developed with tap water from Sofia, Bulgaria, consisting of 1% solutions of Haberlea rhodopensis. A study revealed that during the drying process in H. rhodopensis the number of free water molecules decreases, and water dimers are formed. The aim of our study was to determine the number of water molecules in clusters in 1% solutions of hydrated H. rhodopensis plants. Results were analyzed according to the two types of water used in the experiment. Th EVOdrop device is equipped with an ultranano membrane and rotating jet nozzle to create a vortex water and saturation thanks to a second device EVObooster to obtain hydrogen-rich water. In the current study Hydrogen-rich water is referred to as Hydrogen EVOdrop Water (HEW). Research was conducted using the following methods—spectral methods non-equilibrium energy spectrum (NES) and differential non-equilibrium energy spectrum (DNES), mathematical models, and study of the distribution of water molecules in water clusters. In a licensed Eurotest Laboratory, the research of tap water before and after flowing through the EVOdrop device was proven. Studies have been carried out on the structuring of water molecule clusters after change of hydrogen bond energies. The restructuring comes with rearrangement of water molecules by the energy levels of hydrogen bonds. Local extrema can be observed in the spectrum with largest amount of water molecules. The structural changes were tested using the NES and DNES spectral methods. The conducted research proved that the application of EVOdrop device and EVObooster changes the parameters of water to benefit hydration and health. MDPI 2022-10-10 /pmc/articles/PMC9572004/ /pubmed/36235522 http://dx.doi.org/10.3390/plants11192655 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ignatov, Ignat
Huether, Fabio
Neshev, Nikolai
Kiselova-Kaneva, Yoana
Popova, Teodora P.
Bankova, Ralitsa
Valcheva, Nedyalka
Ignatov, Alexander I.
Angelcheva, Mariana
Angushev, Ivan
Baiti, Sadek
Research of Water Molecules Cluster Structuring during Haberlea rhodopensis Friv. Hydration
title Research of Water Molecules Cluster Structuring during Haberlea rhodopensis Friv. Hydration
title_full Research of Water Molecules Cluster Structuring during Haberlea rhodopensis Friv. Hydration
title_fullStr Research of Water Molecules Cluster Structuring during Haberlea rhodopensis Friv. Hydration
title_full_unstemmed Research of Water Molecules Cluster Structuring during Haberlea rhodopensis Friv. Hydration
title_short Research of Water Molecules Cluster Structuring during Haberlea rhodopensis Friv. Hydration
title_sort research of water molecules cluster structuring during haberlea rhodopensis friv. hydration
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9572004/
https://www.ncbi.nlm.nih.gov/pubmed/36235522
http://dx.doi.org/10.3390/plants11192655
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