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Inflammatory Biomarkers of Extracellular Matrix Remodeling and Disease Activity in Crohn’s Disease and Ulcerative Colitis

Extracellular matrix (ECM) homeostasis is highly affected in active inflammatory bowel disease (IBD). The aim of the study was to investigate serological biomarkers of type III, IV, and V collagen degradation and formation, and their association with disease activity in IBD. ECM remodeling serum bio...

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Autores principales: Domislovic, Viktor, Høg Mortensen, Joachim, Lindholm, Majken, Kaarsdal, Morten Asser, Brinar, Marko, Barisic, Ana, Manon-Jensen, Tina, Krznaric, Zeljko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9572110/
https://www.ncbi.nlm.nih.gov/pubmed/36233775
http://dx.doi.org/10.3390/jcm11195907
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author Domislovic, Viktor
Høg Mortensen, Joachim
Lindholm, Majken
Kaarsdal, Morten Asser
Brinar, Marko
Barisic, Ana
Manon-Jensen, Tina
Krznaric, Zeljko
author_facet Domislovic, Viktor
Høg Mortensen, Joachim
Lindholm, Majken
Kaarsdal, Morten Asser
Brinar, Marko
Barisic, Ana
Manon-Jensen, Tina
Krznaric, Zeljko
author_sort Domislovic, Viktor
collection PubMed
description Extracellular matrix (ECM) homeostasis is highly affected in active inflammatory bowel disease (IBD). The aim of the study was to investigate serological biomarkers of type III, IV, and V collagen degradation and formation, and their association with disease activity in IBD. ECM remodeling serum biomarkers were measured in 162 IBD patients, 110 with Crohn’s disease (CD) and 52 with ulcerative colitis (UC), and in 29 healthy donors. Biomarkers of type III collagen degradation (C3M) and formation (PRO-C3), type IV collagen degradation (C4M) and formation (PRO-C4), and type V collagen formation (PRO-C5) were measured using ELISA. Inflammatory activity was assessed using endoscopic, clinical, and biochemical activity indices. The highest diagnostic value was identified in discriminating endoscopically moderate to severe disease in CD (PRO-C3, C3M/PRO-C3, and C4M with AUC of 0.70, 0.73, and 0.69, respectively) and UC (C3M, C3M/PRO-C3, and C4M with AUC of 0.86, 0.80, and 0.76, respectively). C4M and C3M/PRO-C3 in combination yielded AUC of 0.93 (0.66–0.90) in CD and 0.94 (0.65–0.99) in UC. This study confirmed that ECM remodeling reflected disease activity in CD and UC. A combination of C4M, C3M, and PRO-C3 biomarkers may potentially be considered as a biomarker differentiating moderate to severe endoscopic disease.
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spelling pubmed-95721102022-10-17 Inflammatory Biomarkers of Extracellular Matrix Remodeling and Disease Activity in Crohn’s Disease and Ulcerative Colitis Domislovic, Viktor Høg Mortensen, Joachim Lindholm, Majken Kaarsdal, Morten Asser Brinar, Marko Barisic, Ana Manon-Jensen, Tina Krznaric, Zeljko J Clin Med Article Extracellular matrix (ECM) homeostasis is highly affected in active inflammatory bowel disease (IBD). The aim of the study was to investigate serological biomarkers of type III, IV, and V collagen degradation and formation, and their association with disease activity in IBD. ECM remodeling serum biomarkers were measured in 162 IBD patients, 110 with Crohn’s disease (CD) and 52 with ulcerative colitis (UC), and in 29 healthy donors. Biomarkers of type III collagen degradation (C3M) and formation (PRO-C3), type IV collagen degradation (C4M) and formation (PRO-C4), and type V collagen formation (PRO-C5) were measured using ELISA. Inflammatory activity was assessed using endoscopic, clinical, and biochemical activity indices. The highest diagnostic value was identified in discriminating endoscopically moderate to severe disease in CD (PRO-C3, C3M/PRO-C3, and C4M with AUC of 0.70, 0.73, and 0.69, respectively) and UC (C3M, C3M/PRO-C3, and C4M with AUC of 0.86, 0.80, and 0.76, respectively). C4M and C3M/PRO-C3 in combination yielded AUC of 0.93 (0.66–0.90) in CD and 0.94 (0.65–0.99) in UC. This study confirmed that ECM remodeling reflected disease activity in CD and UC. A combination of C4M, C3M, and PRO-C3 biomarkers may potentially be considered as a biomarker differentiating moderate to severe endoscopic disease. MDPI 2022-10-07 /pmc/articles/PMC9572110/ /pubmed/36233775 http://dx.doi.org/10.3390/jcm11195907 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Domislovic, Viktor
Høg Mortensen, Joachim
Lindholm, Majken
Kaarsdal, Morten Asser
Brinar, Marko
Barisic, Ana
Manon-Jensen, Tina
Krznaric, Zeljko
Inflammatory Biomarkers of Extracellular Matrix Remodeling and Disease Activity in Crohn’s Disease and Ulcerative Colitis
title Inflammatory Biomarkers of Extracellular Matrix Remodeling and Disease Activity in Crohn’s Disease and Ulcerative Colitis
title_full Inflammatory Biomarkers of Extracellular Matrix Remodeling and Disease Activity in Crohn’s Disease and Ulcerative Colitis
title_fullStr Inflammatory Biomarkers of Extracellular Matrix Remodeling and Disease Activity in Crohn’s Disease and Ulcerative Colitis
title_full_unstemmed Inflammatory Biomarkers of Extracellular Matrix Remodeling and Disease Activity in Crohn’s Disease and Ulcerative Colitis
title_short Inflammatory Biomarkers of Extracellular Matrix Remodeling and Disease Activity in Crohn’s Disease and Ulcerative Colitis
title_sort inflammatory biomarkers of extracellular matrix remodeling and disease activity in crohn’s disease and ulcerative colitis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9572110/
https://www.ncbi.nlm.nih.gov/pubmed/36233775
http://dx.doi.org/10.3390/jcm11195907
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