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Fermentation Extract of Naringenin Increases the Expression of Estrogenic Receptor β and Modulates Genes Related to the p53 Signalling Pathway, miR-200c and miR-141 in Human Colon Cancer Cells Exposed to BPA

The estrogenic receptor beta (ERβ) protects against carcinogenesis by stimulating apoptosis. Bisphenol A (BPA) is related to promoting cancer, and naringenin has chemoprotective activities both can bind to ERβ. Naringenin in the colon is metabolized by the microbiota. Cancer involves genetic and epi...

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Autores principales: Lozano-Herrera, Sara Julietta, Luna-Bárcenas, Gabriel, Guevara-González, Ramón Gerardo, Campos-Vega, Rocio, Solís-Sáinz, Juan Carlos, Hernández-Puga, Ana Gabriela, Vergara-Castañeda, Haydé Azeneth
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9572342/
https://www.ncbi.nlm.nih.gov/pubmed/36235125
http://dx.doi.org/10.3390/molecules27196588
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author Lozano-Herrera, Sara Julietta
Luna-Bárcenas, Gabriel
Guevara-González, Ramón Gerardo
Campos-Vega, Rocio
Solís-Sáinz, Juan Carlos
Hernández-Puga, Ana Gabriela
Vergara-Castañeda, Haydé Azeneth
author_facet Lozano-Herrera, Sara Julietta
Luna-Bárcenas, Gabriel
Guevara-González, Ramón Gerardo
Campos-Vega, Rocio
Solís-Sáinz, Juan Carlos
Hernández-Puga, Ana Gabriela
Vergara-Castañeda, Haydé Azeneth
author_sort Lozano-Herrera, Sara Julietta
collection PubMed
description The estrogenic receptor beta (ERβ) protects against carcinogenesis by stimulating apoptosis. Bisphenol A (BPA) is related to promoting cancer, and naringenin has chemoprotective activities both can bind to ERβ. Naringenin in the colon is metabolized by the microbiota. Cancer involves genetic and epigenetic mechanisms, including miRNAs. The objective of the present study was to evaluate the co-exposure effect of colonic in vitro fermented extract of naringenin (FEN) and BPA, to elucidate molecular effects in HT-29 colon cancer cell line. For this, we quantified genes related to the p53 signaling pathway as well as ERβ, miR-200c, and miR-141. As an important result, naringenin (IC50 250 µM) and FEN (IC50 37%) promoted intrinsic pathways of apoptosis through phosphatase and tensin homolog (PTEN) (+2.70, +1.72-fold, respectively) and CASP9 (+3.99, +2.03-fold, respectively) expression. BPA decreased the expression of PTEN (−3.46-fold) gene regulated by miR-200. We suggest that once co-exposed, cells undergo a greater stress forcing them to mediate other extrinsic apoptosis mechanisms associated with death domain FASL. In turn, these findings are related to the increase of ERβ (5.3-fold with naringenin and 13.67-fold with FEN) gene expression, important in the inhibition of carcinogenic development.
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spelling pubmed-95723422022-10-17 Fermentation Extract of Naringenin Increases the Expression of Estrogenic Receptor β and Modulates Genes Related to the p53 Signalling Pathway, miR-200c and miR-141 in Human Colon Cancer Cells Exposed to BPA Lozano-Herrera, Sara Julietta Luna-Bárcenas, Gabriel Guevara-González, Ramón Gerardo Campos-Vega, Rocio Solís-Sáinz, Juan Carlos Hernández-Puga, Ana Gabriela Vergara-Castañeda, Haydé Azeneth Molecules Article The estrogenic receptor beta (ERβ) protects against carcinogenesis by stimulating apoptosis. Bisphenol A (BPA) is related to promoting cancer, and naringenin has chemoprotective activities both can bind to ERβ. Naringenin in the colon is metabolized by the microbiota. Cancer involves genetic and epigenetic mechanisms, including miRNAs. The objective of the present study was to evaluate the co-exposure effect of colonic in vitro fermented extract of naringenin (FEN) and BPA, to elucidate molecular effects in HT-29 colon cancer cell line. For this, we quantified genes related to the p53 signaling pathway as well as ERβ, miR-200c, and miR-141. As an important result, naringenin (IC50 250 µM) and FEN (IC50 37%) promoted intrinsic pathways of apoptosis through phosphatase and tensin homolog (PTEN) (+2.70, +1.72-fold, respectively) and CASP9 (+3.99, +2.03-fold, respectively) expression. BPA decreased the expression of PTEN (−3.46-fold) gene regulated by miR-200. We suggest that once co-exposed, cells undergo a greater stress forcing them to mediate other extrinsic apoptosis mechanisms associated with death domain FASL. In turn, these findings are related to the increase of ERβ (5.3-fold with naringenin and 13.67-fold with FEN) gene expression, important in the inhibition of carcinogenic development. MDPI 2022-10-05 /pmc/articles/PMC9572342/ /pubmed/36235125 http://dx.doi.org/10.3390/molecules27196588 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lozano-Herrera, Sara Julietta
Luna-Bárcenas, Gabriel
Guevara-González, Ramón Gerardo
Campos-Vega, Rocio
Solís-Sáinz, Juan Carlos
Hernández-Puga, Ana Gabriela
Vergara-Castañeda, Haydé Azeneth
Fermentation Extract of Naringenin Increases the Expression of Estrogenic Receptor β and Modulates Genes Related to the p53 Signalling Pathway, miR-200c and miR-141 in Human Colon Cancer Cells Exposed to BPA
title Fermentation Extract of Naringenin Increases the Expression of Estrogenic Receptor β and Modulates Genes Related to the p53 Signalling Pathway, miR-200c and miR-141 in Human Colon Cancer Cells Exposed to BPA
title_full Fermentation Extract of Naringenin Increases the Expression of Estrogenic Receptor β and Modulates Genes Related to the p53 Signalling Pathway, miR-200c and miR-141 in Human Colon Cancer Cells Exposed to BPA
title_fullStr Fermentation Extract of Naringenin Increases the Expression of Estrogenic Receptor β and Modulates Genes Related to the p53 Signalling Pathway, miR-200c and miR-141 in Human Colon Cancer Cells Exposed to BPA
title_full_unstemmed Fermentation Extract of Naringenin Increases the Expression of Estrogenic Receptor β and Modulates Genes Related to the p53 Signalling Pathway, miR-200c and miR-141 in Human Colon Cancer Cells Exposed to BPA
title_short Fermentation Extract of Naringenin Increases the Expression of Estrogenic Receptor β and Modulates Genes Related to the p53 Signalling Pathway, miR-200c and miR-141 in Human Colon Cancer Cells Exposed to BPA
title_sort fermentation extract of naringenin increases the expression of estrogenic receptor β and modulates genes related to the p53 signalling pathway, mir-200c and mir-141 in human colon cancer cells exposed to bpa
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9572342/
https://www.ncbi.nlm.nih.gov/pubmed/36235125
http://dx.doi.org/10.3390/molecules27196588
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