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The Development and Application of Opto-Chemical Tools in the Zebrafish

The zebrafish is one of the most widely adopted animal models in both basic and translational research. This popularity of the zebrafish results from several advantages such as a high degree of similarity to the human genome, the ease of genetic and chemical perturbations, external fertilization wit...

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Autores principales: Feng, Zhiping, Ducos, Bertrand, Scerbo, Pierluigi, Aujard, Isabelle, Jullien, Ludovic, Bensimon, David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9572478/
https://www.ncbi.nlm.nih.gov/pubmed/36234767
http://dx.doi.org/10.3390/molecules27196231
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author Feng, Zhiping
Ducos, Bertrand
Scerbo, Pierluigi
Aujard, Isabelle
Jullien, Ludovic
Bensimon, David
author_facet Feng, Zhiping
Ducos, Bertrand
Scerbo, Pierluigi
Aujard, Isabelle
Jullien, Ludovic
Bensimon, David
author_sort Feng, Zhiping
collection PubMed
description The zebrafish is one of the most widely adopted animal models in both basic and translational research. This popularity of the zebrafish results from several advantages such as a high degree of similarity to the human genome, the ease of genetic and chemical perturbations, external fertilization with high fecundity, transparent and fast-developing embryos, and relatively low cost-effective maintenance. In particular, body translucency is a unique feature of zebrafish that is not adequately obtained with other vertebrate organisms. The animal’s distinctive optical clarity and small size therefore make it a successful model for optical modulation and observation. Furthermore, the convenience of microinjection and high embryonic permeability readily allow for efficient delivery of large and small molecules into live animals. Finally, the numerous number of siblings obtained from a single pair of animals offers large replicates and improved statistical analysis of the results. In this review, we describe the development of opto-chemical tools based on various strategies that control biological activities with unprecedented spatiotemporal resolution. We also discuss the reported applications of these tools in zebrafish and highlight the current challenges and future possibilities of opto-chemical approaches, particularly at the single cell level.
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spelling pubmed-95724782022-10-17 The Development and Application of Opto-Chemical Tools in the Zebrafish Feng, Zhiping Ducos, Bertrand Scerbo, Pierluigi Aujard, Isabelle Jullien, Ludovic Bensimon, David Molecules Review The zebrafish is one of the most widely adopted animal models in both basic and translational research. This popularity of the zebrafish results from several advantages such as a high degree of similarity to the human genome, the ease of genetic and chemical perturbations, external fertilization with high fecundity, transparent and fast-developing embryos, and relatively low cost-effective maintenance. In particular, body translucency is a unique feature of zebrafish that is not adequately obtained with other vertebrate organisms. The animal’s distinctive optical clarity and small size therefore make it a successful model for optical modulation and observation. Furthermore, the convenience of microinjection and high embryonic permeability readily allow for efficient delivery of large and small molecules into live animals. Finally, the numerous number of siblings obtained from a single pair of animals offers large replicates and improved statistical analysis of the results. In this review, we describe the development of opto-chemical tools based on various strategies that control biological activities with unprecedented spatiotemporal resolution. We also discuss the reported applications of these tools in zebrafish and highlight the current challenges and future possibilities of opto-chemical approaches, particularly at the single cell level. MDPI 2022-09-22 /pmc/articles/PMC9572478/ /pubmed/36234767 http://dx.doi.org/10.3390/molecules27196231 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Feng, Zhiping
Ducos, Bertrand
Scerbo, Pierluigi
Aujard, Isabelle
Jullien, Ludovic
Bensimon, David
The Development and Application of Opto-Chemical Tools in the Zebrafish
title The Development and Application of Opto-Chemical Tools in the Zebrafish
title_full The Development and Application of Opto-Chemical Tools in the Zebrafish
title_fullStr The Development and Application of Opto-Chemical Tools in the Zebrafish
title_full_unstemmed The Development and Application of Opto-Chemical Tools in the Zebrafish
title_short The Development and Application of Opto-Chemical Tools in the Zebrafish
title_sort development and application of opto-chemical tools in the zebrafish
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9572478/
https://www.ncbi.nlm.nih.gov/pubmed/36234767
http://dx.doi.org/10.3390/molecules27196231
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