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Silver Nanoparticles Formulation of Flower Head’s Polyphenols of Cynara scolymus L.: A Promising Candidate against Prostate (PC-3) Cancer Cell Line through Apoptosis Activation

Cynara scolymus L. (Family: Compositae) or artichoke is a nutritious edible plant widely used for its hepatoprotective effect. Crude extracts of flower, bract, and stem were prepared and evaluated for their in vitro antioxidant activity and phenolic content. The flower crude extract exhibited the hi...

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Autores principales: Khedr, Amgad I. M., Goda, Marwa S., Farrag, Abdelaziz F. S., Nasr, Ali M., Swidan, Shady A., Nafie, Mohamed S., Abdel-Kader, Maged S., Badr, Jihan M., Abdelhameed, Reda F. A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9572662/
https://www.ncbi.nlm.nih.gov/pubmed/36234842
http://dx.doi.org/10.3390/molecules27196304
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author Khedr, Amgad I. M.
Goda, Marwa S.
Farrag, Abdelaziz F. S.
Nasr, Ali M.
Swidan, Shady A.
Nafie, Mohamed S.
Abdel-Kader, Maged S.
Badr, Jihan M.
Abdelhameed, Reda F. A.
author_facet Khedr, Amgad I. M.
Goda, Marwa S.
Farrag, Abdelaziz F. S.
Nasr, Ali M.
Swidan, Shady A.
Nafie, Mohamed S.
Abdel-Kader, Maged S.
Badr, Jihan M.
Abdelhameed, Reda F. A.
author_sort Khedr, Amgad I. M.
collection PubMed
description Cynara scolymus L. (Family: Compositae) or artichoke is a nutritious edible plant widely used for its hepatoprotective effect. Crude extracts of flower, bract, and stem were prepared and evaluated for their in vitro antioxidant activity and phenolic content. The flower crude extract exhibited the highest phenolic content (74.29 mg GAE/gm) as well as the best in vitro antioxidant activity using total antioxidant capacity (TAC), ferric reducing antioxidant power (FEAP), and 1,1-diphenyl-2-picrylhyazyl (DPPH) scavenging assays compared with ascorbic acid. Phenolic fractions of the crude extracts of different parts were separated and identified using high-performance liquid chromatography HPLC-DAD analysis. The silver nanoparticles of these phenolic fractions were established and tested for their cytotoxicity and apoptotic activity. Results showed that silver nanoparticles of a polyphenolic fraction of flower extract (Nano-TP/Flowers) exhibited potent cytotoxicity against prostate (PC-3) and lung (A549) cancer cell lines with IC(50) values of 0.85 μg/mL and 0.94 μg/mL, respectively, compared with doxorubicin as a standard. For apoptosis-induction, Nano-TP/Flowers exhibited apoptosis in PC-3 with a higher ratio than in A549 cells. It induced total prostate apoptotic cell death by 227-fold change while it induced apoptosis in A549 cells by 15.6-fold change. Nano-TP/Flowers upregulated both pro-apoptotic markers and downregulated the antiapoptotic genes using RT-PCR. Hence, this extract may serve as a promising source for anti-prostate cancer candidates.
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spelling pubmed-95726622022-10-17 Silver Nanoparticles Formulation of Flower Head’s Polyphenols of Cynara scolymus L.: A Promising Candidate against Prostate (PC-3) Cancer Cell Line through Apoptosis Activation Khedr, Amgad I. M. Goda, Marwa S. Farrag, Abdelaziz F. S. Nasr, Ali M. Swidan, Shady A. Nafie, Mohamed S. Abdel-Kader, Maged S. Badr, Jihan M. Abdelhameed, Reda F. A. Molecules Article Cynara scolymus L. (Family: Compositae) or artichoke is a nutritious edible plant widely used for its hepatoprotective effect. Crude extracts of flower, bract, and stem were prepared and evaluated for their in vitro antioxidant activity and phenolic content. The flower crude extract exhibited the highest phenolic content (74.29 mg GAE/gm) as well as the best in vitro antioxidant activity using total antioxidant capacity (TAC), ferric reducing antioxidant power (FEAP), and 1,1-diphenyl-2-picrylhyazyl (DPPH) scavenging assays compared with ascorbic acid. Phenolic fractions of the crude extracts of different parts were separated and identified using high-performance liquid chromatography HPLC-DAD analysis. The silver nanoparticles of these phenolic fractions were established and tested for their cytotoxicity and apoptotic activity. Results showed that silver nanoparticles of a polyphenolic fraction of flower extract (Nano-TP/Flowers) exhibited potent cytotoxicity against prostate (PC-3) and lung (A549) cancer cell lines with IC(50) values of 0.85 μg/mL and 0.94 μg/mL, respectively, compared with doxorubicin as a standard. For apoptosis-induction, Nano-TP/Flowers exhibited apoptosis in PC-3 with a higher ratio than in A549 cells. It induced total prostate apoptotic cell death by 227-fold change while it induced apoptosis in A549 cells by 15.6-fold change. Nano-TP/Flowers upregulated both pro-apoptotic markers and downregulated the antiapoptotic genes using RT-PCR. Hence, this extract may serve as a promising source for anti-prostate cancer candidates. MDPI 2022-09-24 /pmc/articles/PMC9572662/ /pubmed/36234842 http://dx.doi.org/10.3390/molecules27196304 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Khedr, Amgad I. M.
Goda, Marwa S.
Farrag, Abdelaziz F. S.
Nasr, Ali M.
Swidan, Shady A.
Nafie, Mohamed S.
Abdel-Kader, Maged S.
Badr, Jihan M.
Abdelhameed, Reda F. A.
Silver Nanoparticles Formulation of Flower Head’s Polyphenols of Cynara scolymus L.: A Promising Candidate against Prostate (PC-3) Cancer Cell Line through Apoptosis Activation
title Silver Nanoparticles Formulation of Flower Head’s Polyphenols of Cynara scolymus L.: A Promising Candidate against Prostate (PC-3) Cancer Cell Line through Apoptosis Activation
title_full Silver Nanoparticles Formulation of Flower Head’s Polyphenols of Cynara scolymus L.: A Promising Candidate against Prostate (PC-3) Cancer Cell Line through Apoptosis Activation
title_fullStr Silver Nanoparticles Formulation of Flower Head’s Polyphenols of Cynara scolymus L.: A Promising Candidate against Prostate (PC-3) Cancer Cell Line through Apoptosis Activation
title_full_unstemmed Silver Nanoparticles Formulation of Flower Head’s Polyphenols of Cynara scolymus L.: A Promising Candidate against Prostate (PC-3) Cancer Cell Line through Apoptosis Activation
title_short Silver Nanoparticles Formulation of Flower Head’s Polyphenols of Cynara scolymus L.: A Promising Candidate against Prostate (PC-3) Cancer Cell Line through Apoptosis Activation
title_sort silver nanoparticles formulation of flower head’s polyphenols of cynara scolymus l.: a promising candidate against prostate (pc-3) cancer cell line through apoptosis activation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9572662/
https://www.ncbi.nlm.nih.gov/pubmed/36234842
http://dx.doi.org/10.3390/molecules27196304
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