A New Method for Abrin Detection Based on the Interaction between Target Molecules and Fluorescently Labeled Aptamers on Magnetic Microspheres

A quantitative structure–activity relationship (QSAR) model for the structure and affinity of abrin aptamers was established. A higher affinity abrin aptamer based on the established QSAR model was screened by site-directed mutagenesis. The fluorescence quenching effect between magnetic microspheres...

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Autores principales: Liu, Zhiwei, Tong, Zhaoyang, Wu, Yuting, Liu, Bing, Feng, Shasha, Mu, Xihui, Wang, Jiang, Du, Bin, Xu, Jianjie, Liu, Shuai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9573059/
https://www.ncbi.nlm.nih.gov/pubmed/36234322
http://dx.doi.org/10.3390/ma15196977
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author Liu, Zhiwei
Tong, Zhaoyang
Wu, Yuting
Liu, Bing
Feng, Shasha
Mu, Xihui
Wang, Jiang
Du, Bin
Xu, Jianjie
Liu, Shuai
author_facet Liu, Zhiwei
Tong, Zhaoyang
Wu, Yuting
Liu, Bing
Feng, Shasha
Mu, Xihui
Wang, Jiang
Du, Bin
Xu, Jianjie
Liu, Shuai
author_sort Liu, Zhiwei
collection PubMed
description A quantitative structure–activity relationship (QSAR) model for the structure and affinity of abrin aptamers was established. A higher affinity abrin aptamer based on the established QSAR model was screened by site-directed mutagenesis. The fluorescence quenching effect between magnetic microspheres and fluorescent molecules was studied for the first time. A new method for abrin detection based on the interaction between target molecules and fluorescently labeled aptamers on magnetic microspheres was developed, with the detection limit of 5 ng mL(−1). This method can overcome the influence of complex environmental interferents in abrin detection and can meet the analysis requirements for simulated samples such as water, soil, and food.
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spelling pubmed-95730592022-10-17 A New Method for Abrin Detection Based on the Interaction between Target Molecules and Fluorescently Labeled Aptamers on Magnetic Microspheres Liu, Zhiwei Tong, Zhaoyang Wu, Yuting Liu, Bing Feng, Shasha Mu, Xihui Wang, Jiang Du, Bin Xu, Jianjie Liu, Shuai Materials (Basel) Article A quantitative structure–activity relationship (QSAR) model for the structure and affinity of abrin aptamers was established. A higher affinity abrin aptamer based on the established QSAR model was screened by site-directed mutagenesis. The fluorescence quenching effect between magnetic microspheres and fluorescent molecules was studied for the first time. A new method for abrin detection based on the interaction between target molecules and fluorescently labeled aptamers on magnetic microspheres was developed, with the detection limit of 5 ng mL(−1). This method can overcome the influence of complex environmental interferents in abrin detection and can meet the analysis requirements for simulated samples such as water, soil, and food. MDPI 2022-10-08 /pmc/articles/PMC9573059/ /pubmed/36234322 http://dx.doi.org/10.3390/ma15196977 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Zhiwei
Tong, Zhaoyang
Wu, Yuting
Liu, Bing
Feng, Shasha
Mu, Xihui
Wang, Jiang
Du, Bin
Xu, Jianjie
Liu, Shuai
A New Method for Abrin Detection Based on the Interaction between Target Molecules and Fluorescently Labeled Aptamers on Magnetic Microspheres
title A New Method for Abrin Detection Based on the Interaction between Target Molecules and Fluorescently Labeled Aptamers on Magnetic Microspheres
title_full A New Method for Abrin Detection Based on the Interaction between Target Molecules and Fluorescently Labeled Aptamers on Magnetic Microspheres
title_fullStr A New Method for Abrin Detection Based on the Interaction between Target Molecules and Fluorescently Labeled Aptamers on Magnetic Microspheres
title_full_unstemmed A New Method for Abrin Detection Based on the Interaction between Target Molecules and Fluorescently Labeled Aptamers on Magnetic Microspheres
title_short A New Method for Abrin Detection Based on the Interaction between Target Molecules and Fluorescently Labeled Aptamers on Magnetic Microspheres
title_sort new method for abrin detection based on the interaction between target molecules and fluorescently labeled aptamers on magnetic microspheres
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9573059/
https://www.ncbi.nlm.nih.gov/pubmed/36234322
http://dx.doi.org/10.3390/ma15196977
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