Cargando…
Resveratrol in the Hypothalamic Paraventricular Nucleus Attenuates Hypertension by Regulation of ROS and Neurotransmitters
Background: The hypothalamic paraventricular nucleus (PVN) is an important nucleus in the brain that plays a key role in regulating sympathetic nerve activity (SNA) and blood pressure. Silent mating-type information regulation 2 homolog-1 (sirtuin1, SIRT1) not only protects cardiovascular function b...
Autores principales: | , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9573276/ https://www.ncbi.nlm.nih.gov/pubmed/36235829 http://dx.doi.org/10.3390/nu14194177 |
_version_ | 1784810828425330688 |
---|---|
author | Qi, Jie Fu, Li-Yan Liu, Kai-Li Li, Rui-Juan Qiao, Jin-An Yu, Xiao-Jing Yu, Jia-Yue Li, Ying Feng, Zhi-Peng Yi, Qiu-Yue Jia, Hong Gao, Hong-Li Tan, Hong Kang, Yu-Ming |
author_facet | Qi, Jie Fu, Li-Yan Liu, Kai-Li Li, Rui-Juan Qiao, Jin-An Yu, Xiao-Jing Yu, Jia-Yue Li, Ying Feng, Zhi-Peng Yi, Qiu-Yue Jia, Hong Gao, Hong-Li Tan, Hong Kang, Yu-Ming |
author_sort | Qi, Jie |
collection | PubMed |
description | Background: The hypothalamic paraventricular nucleus (PVN) is an important nucleus in the brain that plays a key role in regulating sympathetic nerve activity (SNA) and blood pressure. Silent mating-type information regulation 2 homolog-1 (sirtuin1, SIRT1) not only protects cardiovascular function but also reduces inflammation and oxidative stress in the periphery. However, its role in the central regulation of hypertension remains unknown. It is hypothesized that SIRT1 activation by resveratrol may reduce SNA and lower blood pressure through the regulation of intracellular reactive oxygen species (ROS) and neurotransmitters in the PVN. Methods: The two-kidney one-clip (2K1C) method was used to induce renovascular hypertension in male Sprague-Dawley rats. Then, bilaterally injections of vehicle (artificial cerebrospinal fluid, aCSF, 0.4 μL) or resveratrol (a SIRT1 agonist, 160 μmol/L, 0.4 μL) into rat PVN were performed for four weeks. Results: PVN SIRT1 expression was lower in the hypertension group than the sham surgery (SHAM) group. Activated SIRT1 within the PVN lowered systolic blood pressure and plasma norepinephrine (NE) levels. It was found that PVN of 2K1C animals injected with resveratrol exhibited increased expression of SIRT1, copper-zinc superoxide dismutase (SOD1), and glutamic acid decarboxylase (GAD67), as well as decreased activity of nuclear factor-kappa B (NF-κB) p65 and NAD(P)H oxidase (NOX), particularly NOX4. Treatment with resveratrol also decreased expression of ROS and tyrosine hydroxylase (TH). Conclusion: Resveratrol within the PVN attenuates hypertension via the SIRT1/NF-κB pathway to decrease ROS and restore the balance of excitatory and inhibitory neurotransmitters. |
format | Online Article Text |
id | pubmed-9573276 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-95732762022-10-17 Resveratrol in the Hypothalamic Paraventricular Nucleus Attenuates Hypertension by Regulation of ROS and Neurotransmitters Qi, Jie Fu, Li-Yan Liu, Kai-Li Li, Rui-Juan Qiao, Jin-An Yu, Xiao-Jing Yu, Jia-Yue Li, Ying Feng, Zhi-Peng Yi, Qiu-Yue Jia, Hong Gao, Hong-Li Tan, Hong Kang, Yu-Ming Nutrients Article Background: The hypothalamic paraventricular nucleus (PVN) is an important nucleus in the brain that plays a key role in regulating sympathetic nerve activity (SNA) and blood pressure. Silent mating-type information regulation 2 homolog-1 (sirtuin1, SIRT1) not only protects cardiovascular function but also reduces inflammation and oxidative stress in the periphery. However, its role in the central regulation of hypertension remains unknown. It is hypothesized that SIRT1 activation by resveratrol may reduce SNA and lower blood pressure through the regulation of intracellular reactive oxygen species (ROS) and neurotransmitters in the PVN. Methods: The two-kidney one-clip (2K1C) method was used to induce renovascular hypertension in male Sprague-Dawley rats. Then, bilaterally injections of vehicle (artificial cerebrospinal fluid, aCSF, 0.4 μL) or resveratrol (a SIRT1 agonist, 160 μmol/L, 0.4 μL) into rat PVN were performed for four weeks. Results: PVN SIRT1 expression was lower in the hypertension group than the sham surgery (SHAM) group. Activated SIRT1 within the PVN lowered systolic blood pressure and plasma norepinephrine (NE) levels. It was found that PVN of 2K1C animals injected with resveratrol exhibited increased expression of SIRT1, copper-zinc superoxide dismutase (SOD1), and glutamic acid decarboxylase (GAD67), as well as decreased activity of nuclear factor-kappa B (NF-κB) p65 and NAD(P)H oxidase (NOX), particularly NOX4. Treatment with resveratrol also decreased expression of ROS and tyrosine hydroxylase (TH). Conclusion: Resveratrol within the PVN attenuates hypertension via the SIRT1/NF-κB pathway to decrease ROS and restore the balance of excitatory and inhibitory neurotransmitters. MDPI 2022-10-07 /pmc/articles/PMC9573276/ /pubmed/36235829 http://dx.doi.org/10.3390/nu14194177 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Qi, Jie Fu, Li-Yan Liu, Kai-Li Li, Rui-Juan Qiao, Jin-An Yu, Xiao-Jing Yu, Jia-Yue Li, Ying Feng, Zhi-Peng Yi, Qiu-Yue Jia, Hong Gao, Hong-Li Tan, Hong Kang, Yu-Ming Resveratrol in the Hypothalamic Paraventricular Nucleus Attenuates Hypertension by Regulation of ROS and Neurotransmitters |
title | Resveratrol in the Hypothalamic Paraventricular Nucleus Attenuates Hypertension by Regulation of ROS and Neurotransmitters |
title_full | Resveratrol in the Hypothalamic Paraventricular Nucleus Attenuates Hypertension by Regulation of ROS and Neurotransmitters |
title_fullStr | Resveratrol in the Hypothalamic Paraventricular Nucleus Attenuates Hypertension by Regulation of ROS and Neurotransmitters |
title_full_unstemmed | Resveratrol in the Hypothalamic Paraventricular Nucleus Attenuates Hypertension by Regulation of ROS and Neurotransmitters |
title_short | Resveratrol in the Hypothalamic Paraventricular Nucleus Attenuates Hypertension by Regulation of ROS and Neurotransmitters |
title_sort | resveratrol in the hypothalamic paraventricular nucleus attenuates hypertension by regulation of ros and neurotransmitters |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9573276/ https://www.ncbi.nlm.nih.gov/pubmed/36235829 http://dx.doi.org/10.3390/nu14194177 |
work_keys_str_mv | AT qijie resveratrolinthehypothalamicparaventricularnucleusattenuateshypertensionbyregulationofrosandneurotransmitters AT fuliyan resveratrolinthehypothalamicparaventricularnucleusattenuateshypertensionbyregulationofrosandneurotransmitters AT liukaili resveratrolinthehypothalamicparaventricularnucleusattenuateshypertensionbyregulationofrosandneurotransmitters AT liruijuan resveratrolinthehypothalamicparaventricularnucleusattenuateshypertensionbyregulationofrosandneurotransmitters AT qiaojinan resveratrolinthehypothalamicparaventricularnucleusattenuateshypertensionbyregulationofrosandneurotransmitters AT yuxiaojing resveratrolinthehypothalamicparaventricularnucleusattenuateshypertensionbyregulationofrosandneurotransmitters AT yujiayue resveratrolinthehypothalamicparaventricularnucleusattenuateshypertensionbyregulationofrosandneurotransmitters AT liying resveratrolinthehypothalamicparaventricularnucleusattenuateshypertensionbyregulationofrosandneurotransmitters AT fengzhipeng resveratrolinthehypothalamicparaventricularnucleusattenuateshypertensionbyregulationofrosandneurotransmitters AT yiqiuyue resveratrolinthehypothalamicparaventricularnucleusattenuateshypertensionbyregulationofrosandneurotransmitters AT jiahong resveratrolinthehypothalamicparaventricularnucleusattenuateshypertensionbyregulationofrosandneurotransmitters AT gaohongli resveratrolinthehypothalamicparaventricularnucleusattenuateshypertensionbyregulationofrosandneurotransmitters AT tanhong resveratrolinthehypothalamicparaventricularnucleusattenuateshypertensionbyregulationofrosandneurotransmitters AT kangyuming resveratrolinthehypothalamicparaventricularnucleusattenuateshypertensionbyregulationofrosandneurotransmitters |