Cargando…

Resveratrol in the Hypothalamic Paraventricular Nucleus Attenuates Hypertension by Regulation of ROS and Neurotransmitters

Background: The hypothalamic paraventricular nucleus (PVN) is an important nucleus in the brain that plays a key role in regulating sympathetic nerve activity (SNA) and blood pressure. Silent mating-type information regulation 2 homolog-1 (sirtuin1, SIRT1) not only protects cardiovascular function b...

Descripción completa

Detalles Bibliográficos
Autores principales: Qi, Jie, Fu, Li-Yan, Liu, Kai-Li, Li, Rui-Juan, Qiao, Jin-An, Yu, Xiao-Jing, Yu, Jia-Yue, Li, Ying, Feng, Zhi-Peng, Yi, Qiu-Yue, Jia, Hong, Gao, Hong-Li, Tan, Hong, Kang, Yu-Ming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9573276/
https://www.ncbi.nlm.nih.gov/pubmed/36235829
http://dx.doi.org/10.3390/nu14194177
_version_ 1784810828425330688
author Qi, Jie
Fu, Li-Yan
Liu, Kai-Li
Li, Rui-Juan
Qiao, Jin-An
Yu, Xiao-Jing
Yu, Jia-Yue
Li, Ying
Feng, Zhi-Peng
Yi, Qiu-Yue
Jia, Hong
Gao, Hong-Li
Tan, Hong
Kang, Yu-Ming
author_facet Qi, Jie
Fu, Li-Yan
Liu, Kai-Li
Li, Rui-Juan
Qiao, Jin-An
Yu, Xiao-Jing
Yu, Jia-Yue
Li, Ying
Feng, Zhi-Peng
Yi, Qiu-Yue
Jia, Hong
Gao, Hong-Li
Tan, Hong
Kang, Yu-Ming
author_sort Qi, Jie
collection PubMed
description Background: The hypothalamic paraventricular nucleus (PVN) is an important nucleus in the brain that plays a key role in regulating sympathetic nerve activity (SNA) and blood pressure. Silent mating-type information regulation 2 homolog-1 (sirtuin1, SIRT1) not only protects cardiovascular function but also reduces inflammation and oxidative stress in the periphery. However, its role in the central regulation of hypertension remains unknown. It is hypothesized that SIRT1 activation by resveratrol may reduce SNA and lower blood pressure through the regulation of intracellular reactive oxygen species (ROS) and neurotransmitters in the PVN. Methods: The two-kidney one-clip (2K1C) method was used to induce renovascular hypertension in male Sprague-Dawley rats. Then, bilaterally injections of vehicle (artificial cerebrospinal fluid, aCSF, 0.4 μL) or resveratrol (a SIRT1 agonist, 160 μmol/L, 0.4 μL) into rat PVN were performed for four weeks. Results: PVN SIRT1 expression was lower in the hypertension group than the sham surgery (SHAM) group. Activated SIRT1 within the PVN lowered systolic blood pressure and plasma norepinephrine (NE) levels. It was found that PVN of 2K1C animals injected with resveratrol exhibited increased expression of SIRT1, copper-zinc superoxide dismutase (SOD1), and glutamic acid decarboxylase (GAD67), as well as decreased activity of nuclear factor-kappa B (NF-κB) p65 and NAD(P)H oxidase (NOX), particularly NOX4. Treatment with resveratrol also decreased expression of ROS and tyrosine hydroxylase (TH). Conclusion: Resveratrol within the PVN attenuates hypertension via the SIRT1/NF-κB pathway to decrease ROS and restore the balance of excitatory and inhibitory neurotransmitters.
format Online
Article
Text
id pubmed-9573276
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-95732762022-10-17 Resveratrol in the Hypothalamic Paraventricular Nucleus Attenuates Hypertension by Regulation of ROS and Neurotransmitters Qi, Jie Fu, Li-Yan Liu, Kai-Li Li, Rui-Juan Qiao, Jin-An Yu, Xiao-Jing Yu, Jia-Yue Li, Ying Feng, Zhi-Peng Yi, Qiu-Yue Jia, Hong Gao, Hong-Li Tan, Hong Kang, Yu-Ming Nutrients Article Background: The hypothalamic paraventricular nucleus (PVN) is an important nucleus in the brain that plays a key role in regulating sympathetic nerve activity (SNA) and blood pressure. Silent mating-type information regulation 2 homolog-1 (sirtuin1, SIRT1) not only protects cardiovascular function but also reduces inflammation and oxidative stress in the periphery. However, its role in the central regulation of hypertension remains unknown. It is hypothesized that SIRT1 activation by resveratrol may reduce SNA and lower blood pressure through the regulation of intracellular reactive oxygen species (ROS) and neurotransmitters in the PVN. Methods: The two-kidney one-clip (2K1C) method was used to induce renovascular hypertension in male Sprague-Dawley rats. Then, bilaterally injections of vehicle (artificial cerebrospinal fluid, aCSF, 0.4 μL) or resveratrol (a SIRT1 agonist, 160 μmol/L, 0.4 μL) into rat PVN were performed for four weeks. Results: PVN SIRT1 expression was lower in the hypertension group than the sham surgery (SHAM) group. Activated SIRT1 within the PVN lowered systolic blood pressure and plasma norepinephrine (NE) levels. It was found that PVN of 2K1C animals injected with resveratrol exhibited increased expression of SIRT1, copper-zinc superoxide dismutase (SOD1), and glutamic acid decarboxylase (GAD67), as well as decreased activity of nuclear factor-kappa B (NF-κB) p65 and NAD(P)H oxidase (NOX), particularly NOX4. Treatment with resveratrol also decreased expression of ROS and tyrosine hydroxylase (TH). Conclusion: Resveratrol within the PVN attenuates hypertension via the SIRT1/NF-κB pathway to decrease ROS and restore the balance of excitatory and inhibitory neurotransmitters. MDPI 2022-10-07 /pmc/articles/PMC9573276/ /pubmed/36235829 http://dx.doi.org/10.3390/nu14194177 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Qi, Jie
Fu, Li-Yan
Liu, Kai-Li
Li, Rui-Juan
Qiao, Jin-An
Yu, Xiao-Jing
Yu, Jia-Yue
Li, Ying
Feng, Zhi-Peng
Yi, Qiu-Yue
Jia, Hong
Gao, Hong-Li
Tan, Hong
Kang, Yu-Ming
Resveratrol in the Hypothalamic Paraventricular Nucleus Attenuates Hypertension by Regulation of ROS and Neurotransmitters
title Resveratrol in the Hypothalamic Paraventricular Nucleus Attenuates Hypertension by Regulation of ROS and Neurotransmitters
title_full Resveratrol in the Hypothalamic Paraventricular Nucleus Attenuates Hypertension by Regulation of ROS and Neurotransmitters
title_fullStr Resveratrol in the Hypothalamic Paraventricular Nucleus Attenuates Hypertension by Regulation of ROS and Neurotransmitters
title_full_unstemmed Resveratrol in the Hypothalamic Paraventricular Nucleus Attenuates Hypertension by Regulation of ROS and Neurotransmitters
title_short Resveratrol in the Hypothalamic Paraventricular Nucleus Attenuates Hypertension by Regulation of ROS and Neurotransmitters
title_sort resveratrol in the hypothalamic paraventricular nucleus attenuates hypertension by regulation of ros and neurotransmitters
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9573276/
https://www.ncbi.nlm.nih.gov/pubmed/36235829
http://dx.doi.org/10.3390/nu14194177
work_keys_str_mv AT qijie resveratrolinthehypothalamicparaventricularnucleusattenuateshypertensionbyregulationofrosandneurotransmitters
AT fuliyan resveratrolinthehypothalamicparaventricularnucleusattenuateshypertensionbyregulationofrosandneurotransmitters
AT liukaili resveratrolinthehypothalamicparaventricularnucleusattenuateshypertensionbyregulationofrosandneurotransmitters
AT liruijuan resveratrolinthehypothalamicparaventricularnucleusattenuateshypertensionbyregulationofrosandneurotransmitters
AT qiaojinan resveratrolinthehypothalamicparaventricularnucleusattenuateshypertensionbyregulationofrosandneurotransmitters
AT yuxiaojing resveratrolinthehypothalamicparaventricularnucleusattenuateshypertensionbyregulationofrosandneurotransmitters
AT yujiayue resveratrolinthehypothalamicparaventricularnucleusattenuateshypertensionbyregulationofrosandneurotransmitters
AT liying resveratrolinthehypothalamicparaventricularnucleusattenuateshypertensionbyregulationofrosandneurotransmitters
AT fengzhipeng resveratrolinthehypothalamicparaventricularnucleusattenuateshypertensionbyregulationofrosandneurotransmitters
AT yiqiuyue resveratrolinthehypothalamicparaventricularnucleusattenuateshypertensionbyregulationofrosandneurotransmitters
AT jiahong resveratrolinthehypothalamicparaventricularnucleusattenuateshypertensionbyregulationofrosandneurotransmitters
AT gaohongli resveratrolinthehypothalamicparaventricularnucleusattenuateshypertensionbyregulationofrosandneurotransmitters
AT tanhong resveratrolinthehypothalamicparaventricularnucleusattenuateshypertensionbyregulationofrosandneurotransmitters
AT kangyuming resveratrolinthehypothalamicparaventricularnucleusattenuateshypertensionbyregulationofrosandneurotransmitters