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Sequential Injection Amperometric System Coupling with Bioreactor for In-Line Glucose Monitoring in Cell Culture Application

We proposed a specially designed sequential injection (SI) amperometric system coupling with a bioreactor for in-line glucose monitoring in cell culture. The system is composed of three main parts which are the bioreactor, SI system, and electrochemical detection unit. The bioreactor accommodates si...

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Autores principales: Wongsa, Chanyanut, Udomsom, Suruk, Budwong, Apiwat, Kiwfo, Kanokwan, Grudpan, Kate, Paengnakorn, Pathinan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9573359/
https://www.ncbi.nlm.nih.gov/pubmed/36235202
http://dx.doi.org/10.3390/molecules27196665
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author Wongsa, Chanyanut
Udomsom, Suruk
Budwong, Apiwat
Kiwfo, Kanokwan
Grudpan, Kate
Paengnakorn, Pathinan
author_facet Wongsa, Chanyanut
Udomsom, Suruk
Budwong, Apiwat
Kiwfo, Kanokwan
Grudpan, Kate
Paengnakorn, Pathinan
author_sort Wongsa, Chanyanut
collection PubMed
description We proposed a specially designed sequential injection (SI) amperometric system coupling with a bioreactor for in-line glucose monitoring in cell culture. The system is composed of three main parts which are the bioreactor, SI system, and electrochemical detection unit. The bioreactor accommodates six individual cell culture units which can be operated separately under different conditions. The SI system enables automatic in-line sampling and in-line sample dilution, with a specially designed mixing unit; therefore, it has the benefits of fast analysis time and less contamination risk. The use of 3D-printed microfluidic components, a mixing channel, and a flow cell helped to reduce operational time and sample volume. A disposable screen-printed electrode (SPE), modified with glucose oxidase (GOD), carbon nanotube, and gold nanoparticle, was used for detection. The developed system provided a linear range up to 3.8 mM glucose in cell culture media. In order to work with cell culture in higher glucose media, the in-line sample dilution can be applied. The developed SI system was demonstrated with mouse fibroblast (L929) cell culture. The results show that glucose concentration obtained from the SI system is comparable with that obtained from the conventional colorimetric method. This work can be further developed and applied for in vitro cell-based experiments in biomedical research.
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spelling pubmed-95733592022-10-17 Sequential Injection Amperometric System Coupling with Bioreactor for In-Line Glucose Monitoring in Cell Culture Application Wongsa, Chanyanut Udomsom, Suruk Budwong, Apiwat Kiwfo, Kanokwan Grudpan, Kate Paengnakorn, Pathinan Molecules Article We proposed a specially designed sequential injection (SI) amperometric system coupling with a bioreactor for in-line glucose monitoring in cell culture. The system is composed of three main parts which are the bioreactor, SI system, and electrochemical detection unit. The bioreactor accommodates six individual cell culture units which can be operated separately under different conditions. The SI system enables automatic in-line sampling and in-line sample dilution, with a specially designed mixing unit; therefore, it has the benefits of fast analysis time and less contamination risk. The use of 3D-printed microfluidic components, a mixing channel, and a flow cell helped to reduce operational time and sample volume. A disposable screen-printed electrode (SPE), modified with glucose oxidase (GOD), carbon nanotube, and gold nanoparticle, was used for detection. The developed system provided a linear range up to 3.8 mM glucose in cell culture media. In order to work with cell culture in higher glucose media, the in-line sample dilution can be applied. The developed SI system was demonstrated with mouse fibroblast (L929) cell culture. The results show that glucose concentration obtained from the SI system is comparable with that obtained from the conventional colorimetric method. This work can be further developed and applied for in vitro cell-based experiments in biomedical research. MDPI 2022-10-07 /pmc/articles/PMC9573359/ /pubmed/36235202 http://dx.doi.org/10.3390/molecules27196665 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wongsa, Chanyanut
Udomsom, Suruk
Budwong, Apiwat
Kiwfo, Kanokwan
Grudpan, Kate
Paengnakorn, Pathinan
Sequential Injection Amperometric System Coupling with Bioreactor for In-Line Glucose Monitoring in Cell Culture Application
title Sequential Injection Amperometric System Coupling with Bioreactor for In-Line Glucose Monitoring in Cell Culture Application
title_full Sequential Injection Amperometric System Coupling with Bioreactor for In-Line Glucose Monitoring in Cell Culture Application
title_fullStr Sequential Injection Amperometric System Coupling with Bioreactor for In-Line Glucose Monitoring in Cell Culture Application
title_full_unstemmed Sequential Injection Amperometric System Coupling with Bioreactor for In-Line Glucose Monitoring in Cell Culture Application
title_short Sequential Injection Amperometric System Coupling with Bioreactor for In-Line Glucose Monitoring in Cell Culture Application
title_sort sequential injection amperometric system coupling with bioreactor for in-line glucose monitoring in cell culture application
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9573359/
https://www.ncbi.nlm.nih.gov/pubmed/36235202
http://dx.doi.org/10.3390/molecules27196665
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