Cargando…
Shedding of the Endothelial Glycocalyx Independent of Systemic Tryptase Release during Oncologic Oral Surgery: An Observational Study
The endothelial glycocalyx and endothelial surface layer are crucial for several functions of the vasculature. Damage to the glycocalyx (“shedding”) occurs during diverse clinical conditions, including major surgery. Mast cell tryptase has been proposed as one possible “sheddase”. During oncologic o...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9573529/ https://www.ncbi.nlm.nih.gov/pubmed/36233665 http://dx.doi.org/10.3390/jcm11195797 |
_version_ | 1784810893652000768 |
---|---|
author | Drinhaus, Hendrik Schroeder, Daniel C. Hunzelmann, Nicolas Herff, Holger Annecke, Thorsten Böttiger, Bernd W. Wetsch, Wolfgang A. |
author_facet | Drinhaus, Hendrik Schroeder, Daniel C. Hunzelmann, Nicolas Herff, Holger Annecke, Thorsten Böttiger, Bernd W. Wetsch, Wolfgang A. |
author_sort | Drinhaus, Hendrik |
collection | PubMed |
description | The endothelial glycocalyx and endothelial surface layer are crucial for several functions of the vasculature. Damage to the glycocalyx (“shedding”) occurs during diverse clinical conditions, including major surgery. Mast cell tryptase has been proposed as one possible “sheddase”. During oncologic oral surgery, glycocalyx shedding could be detrimental due to loss of vascular barrier function and consequent oedema in the musculocutaneous flap graft. Concentrations of the glycocalyx components heparan sulphate and syndecan-1, as well as of tryptase in blood serum before and after surgery, were measured in 16 patients undergoing oncologic oral surgery. Secondary measures were the concentrations of these substances on postoperative days 1 and 2. Heparan sulphate rose from 692 (median, interquartile range: 535–845) to 810 (638–963) ng/mL during surgery. Syndecan-1 increased from 35 (22–77) ng/mL to 138 (71–192) ng/mL. Tryptase remained virtually unchanged with 4.2 (3–5.6) before and 4.2 (2.5–5.5) ng/mL after surgery. Concentrations of heparan sulphate and syndecan-1 in serum increased during surgery, indicating glycocalyx shedding. Tryptase concentration remained equal, suggesting other sheddases than systemic tryptase release to be responsible for damage to the glycocalyx. Investigating strategies to protect the glycocalyx during oncologic oral surgery might hold potential to improve flap viability and patient outcome. |
format | Online Article Text |
id | pubmed-9573529 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-95735292022-10-17 Shedding of the Endothelial Glycocalyx Independent of Systemic Tryptase Release during Oncologic Oral Surgery: An Observational Study Drinhaus, Hendrik Schroeder, Daniel C. Hunzelmann, Nicolas Herff, Holger Annecke, Thorsten Böttiger, Bernd W. Wetsch, Wolfgang A. J Clin Med Article The endothelial glycocalyx and endothelial surface layer are crucial for several functions of the vasculature. Damage to the glycocalyx (“shedding”) occurs during diverse clinical conditions, including major surgery. Mast cell tryptase has been proposed as one possible “sheddase”. During oncologic oral surgery, glycocalyx shedding could be detrimental due to loss of vascular barrier function and consequent oedema in the musculocutaneous flap graft. Concentrations of the glycocalyx components heparan sulphate and syndecan-1, as well as of tryptase in blood serum before and after surgery, were measured in 16 patients undergoing oncologic oral surgery. Secondary measures were the concentrations of these substances on postoperative days 1 and 2. Heparan sulphate rose from 692 (median, interquartile range: 535–845) to 810 (638–963) ng/mL during surgery. Syndecan-1 increased from 35 (22–77) ng/mL to 138 (71–192) ng/mL. Tryptase remained virtually unchanged with 4.2 (3–5.6) before and 4.2 (2.5–5.5) ng/mL after surgery. Concentrations of heparan sulphate and syndecan-1 in serum increased during surgery, indicating glycocalyx shedding. Tryptase concentration remained equal, suggesting other sheddases than systemic tryptase release to be responsible for damage to the glycocalyx. Investigating strategies to protect the glycocalyx during oncologic oral surgery might hold potential to improve flap viability and patient outcome. MDPI 2022-09-30 /pmc/articles/PMC9573529/ /pubmed/36233665 http://dx.doi.org/10.3390/jcm11195797 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Drinhaus, Hendrik Schroeder, Daniel C. Hunzelmann, Nicolas Herff, Holger Annecke, Thorsten Böttiger, Bernd W. Wetsch, Wolfgang A. Shedding of the Endothelial Glycocalyx Independent of Systemic Tryptase Release during Oncologic Oral Surgery: An Observational Study |
title | Shedding of the Endothelial Glycocalyx Independent of Systemic Tryptase Release during Oncologic Oral Surgery: An Observational Study |
title_full | Shedding of the Endothelial Glycocalyx Independent of Systemic Tryptase Release during Oncologic Oral Surgery: An Observational Study |
title_fullStr | Shedding of the Endothelial Glycocalyx Independent of Systemic Tryptase Release during Oncologic Oral Surgery: An Observational Study |
title_full_unstemmed | Shedding of the Endothelial Glycocalyx Independent of Systemic Tryptase Release during Oncologic Oral Surgery: An Observational Study |
title_short | Shedding of the Endothelial Glycocalyx Independent of Systemic Tryptase Release during Oncologic Oral Surgery: An Observational Study |
title_sort | shedding of the endothelial glycocalyx independent of systemic tryptase release during oncologic oral surgery: an observational study |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9573529/ https://www.ncbi.nlm.nih.gov/pubmed/36233665 http://dx.doi.org/10.3390/jcm11195797 |
work_keys_str_mv | AT drinhaushendrik sheddingoftheendothelialglycocalyxindependentofsystemictryptasereleaseduringoncologicoralsurgeryanobservationalstudy AT schroederdanielc sheddingoftheendothelialglycocalyxindependentofsystemictryptasereleaseduringoncologicoralsurgeryanobservationalstudy AT hunzelmannnicolas sheddingoftheendothelialglycocalyxindependentofsystemictryptasereleaseduringoncologicoralsurgeryanobservationalstudy AT herffholger sheddingoftheendothelialglycocalyxindependentofsystemictryptasereleaseduringoncologicoralsurgeryanobservationalstudy AT anneckethorsten sheddingoftheendothelialglycocalyxindependentofsystemictryptasereleaseduringoncologicoralsurgeryanobservationalstudy AT bottigerberndw sheddingoftheendothelialglycocalyxindependentofsystemictryptasereleaseduringoncologicoralsurgeryanobservationalstudy AT wetschwolfganga sheddingoftheendothelialglycocalyxindependentofsystemictryptasereleaseduringoncologicoralsurgeryanobservationalstudy |