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Effect of Germination on the Avenanthramide Content of Oats and Their in Vitro Antisensitivity Activities

In this study, a method, based on an ultraperformance liquid chromatography coupled with high-field quadrupole orbitrap high-resolution mass spectrometry (UHPLC-QE-HF-HRMS) platform, was established for the trace determination of three major avenanthramides (AVNs). The MS conditions for determining...

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Autores principales: Feng, Yuchao, Suo, Decheng, Guan, Xin, Wang, Shi, Xiao, Zhiming, Li, Yang, Liu, Xiaolu, Fan, Xia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9573532/
https://www.ncbi.nlm.nih.gov/pubmed/36234703
http://dx.doi.org/10.3390/molecules27196167
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author Feng, Yuchao
Suo, Decheng
Guan, Xin
Wang, Shi
Xiao, Zhiming
Li, Yang
Liu, Xiaolu
Fan, Xia
author_facet Feng, Yuchao
Suo, Decheng
Guan, Xin
Wang, Shi
Xiao, Zhiming
Li, Yang
Liu, Xiaolu
Fan, Xia
author_sort Feng, Yuchao
collection PubMed
description In this study, a method, based on an ultraperformance liquid chromatography coupled with high-field quadrupole orbitrap high-resolution mass spectrometry (UHPLC-QE-HF-HRMS) platform, was established for the trace determination of three major avenanthramides (AVNs). The MS conditions for determining the AVNs were optimized, and the cracking methods of avenanthramides were analyzed. The linear range of the results and the correlation coefficient were 1–2000 μg/L and >0.996, respectively. Further, the established method was employed for the determination of the AVN contents of oats at different germination times, and the results indicated that the AVN contents of Zaohua and Bayou oats increased 19.26 and 6.09 times, respectively, after germination. The total AVN content of both oat varieties reached a maximum on the fifth day of germination (153.51 ± 4.08 and 126.30 ± 3.33 μg/g for the Zaohua and Bayou oats, respectively). Furthermore, this study investigated the antiallergic and antioxidant activities of the germinated oats via hyaluronidase inhibition and 2,2-diphenyl-1-picrylhydrazyl (DPPH)-scavenging assays. The antiallergic and DPPH-scavenging abilities of the ungerminated forms of both oat varieties were weaker. However, on the fifth day of germination, the inhibition rate of anthranilamide hyaluronidase reached 72.7% and 67.3% for the Zaohua and Bayou oat varieties, respectively. The antiallergic abilities of the oats increased significantly on the fifth day of germination in terms of their antiallergic capacities and DPPH clearance (82.67% and 77.64% for the Zaohua and Bayou oats, respectively), and the two indicators exhibited similar trends. These findings demonstrated that AVNs exhibit good antisensitivity and antioxidation properties, and the antisensitivity effect correlated positively with the AVN content.
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spelling pubmed-95735322022-10-17 Effect of Germination on the Avenanthramide Content of Oats and Their in Vitro Antisensitivity Activities Feng, Yuchao Suo, Decheng Guan, Xin Wang, Shi Xiao, Zhiming Li, Yang Liu, Xiaolu Fan, Xia Molecules Article In this study, a method, based on an ultraperformance liquid chromatography coupled with high-field quadrupole orbitrap high-resolution mass spectrometry (UHPLC-QE-HF-HRMS) platform, was established for the trace determination of three major avenanthramides (AVNs). The MS conditions for determining the AVNs were optimized, and the cracking methods of avenanthramides were analyzed. The linear range of the results and the correlation coefficient were 1–2000 μg/L and >0.996, respectively. Further, the established method was employed for the determination of the AVN contents of oats at different germination times, and the results indicated that the AVN contents of Zaohua and Bayou oats increased 19.26 and 6.09 times, respectively, after germination. The total AVN content of both oat varieties reached a maximum on the fifth day of germination (153.51 ± 4.08 and 126.30 ± 3.33 μg/g for the Zaohua and Bayou oats, respectively). Furthermore, this study investigated the antiallergic and antioxidant activities of the germinated oats via hyaluronidase inhibition and 2,2-diphenyl-1-picrylhydrazyl (DPPH)-scavenging assays. The antiallergic and DPPH-scavenging abilities of the ungerminated forms of both oat varieties were weaker. However, on the fifth day of germination, the inhibition rate of anthranilamide hyaluronidase reached 72.7% and 67.3% for the Zaohua and Bayou oat varieties, respectively. The antiallergic abilities of the oats increased significantly on the fifth day of germination in terms of their antiallergic capacities and DPPH clearance (82.67% and 77.64% for the Zaohua and Bayou oats, respectively), and the two indicators exhibited similar trends. These findings demonstrated that AVNs exhibit good antisensitivity and antioxidation properties, and the antisensitivity effect correlated positively with the AVN content. MDPI 2022-09-20 /pmc/articles/PMC9573532/ /pubmed/36234703 http://dx.doi.org/10.3390/molecules27196167 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Feng, Yuchao
Suo, Decheng
Guan, Xin
Wang, Shi
Xiao, Zhiming
Li, Yang
Liu, Xiaolu
Fan, Xia
Effect of Germination on the Avenanthramide Content of Oats and Their in Vitro Antisensitivity Activities
title Effect of Germination on the Avenanthramide Content of Oats and Their in Vitro Antisensitivity Activities
title_full Effect of Germination on the Avenanthramide Content of Oats and Their in Vitro Antisensitivity Activities
title_fullStr Effect of Germination on the Avenanthramide Content of Oats and Their in Vitro Antisensitivity Activities
title_full_unstemmed Effect of Germination on the Avenanthramide Content of Oats and Their in Vitro Antisensitivity Activities
title_short Effect of Germination on the Avenanthramide Content of Oats and Their in Vitro Antisensitivity Activities
title_sort effect of germination on the avenanthramide content of oats and their in vitro antisensitivity activities
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9573532/
https://www.ncbi.nlm.nih.gov/pubmed/36234703
http://dx.doi.org/10.3390/molecules27196167
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