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Large-Scale Heat-Tolerance Screening and Genetic Diversity of Pea (Pisum sativum L.) Germplasms

Pea (Pisum sativum L.) is an important legume crop. However, the yield of pea is adversely affected by heat stress in China. In this study, heat-tolerant germplasms were screened and evaluated in the field under multi-conditions. The results showed that heat stress could significantly affect pea yie...

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Autores principales: Wang, Dong, Yang, Tao, Liu, Rong, Li, Nana, Ahmad, Naveed, Li, Guan, Ji, Yishan, Wang, Chenyu, Li, Mengwei, Yan, Xin, Ding, Hanfeng, Zong, Xuxiao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9573610/
https://www.ncbi.nlm.nih.gov/pubmed/36235339
http://dx.doi.org/10.3390/plants11192473
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author Wang, Dong
Yang, Tao
Liu, Rong
Li, Nana
Ahmad, Naveed
Li, Guan
Ji, Yishan
Wang, Chenyu
Li, Mengwei
Yan, Xin
Ding, Hanfeng
Zong, Xuxiao
author_facet Wang, Dong
Yang, Tao
Liu, Rong
Li, Nana
Ahmad, Naveed
Li, Guan
Ji, Yishan
Wang, Chenyu
Li, Mengwei
Yan, Xin
Ding, Hanfeng
Zong, Xuxiao
author_sort Wang, Dong
collection PubMed
description Pea (Pisum sativum L.) is an important legume crop. However, the yield of pea is adversely affected by heat stress in China. In this study, heat-tolerant germplasms were screened and evaluated in the field under multi-conditions. The results showed that heat stress could significantly affect pea yield. On the basis of grain weight per plant, 257 heat-tolerant and 175 heat-sensitive accessions were obtained from the first year’s screening, and 26 extremely heat-tolerant and 19 extremely heat-sensitive accessions were finally obtained in this study. Based on SNaPshot technology, two sets of SNP markers, including 46 neutral and 20 heat-tolerance-related markers, were used to evaluate the genetic diversity and population genetic structure of the 432 pea accessions obtained from the first year’s screening. Genetic diversity analysis showed that the average polymorphic information content was lower using heat-tolerance-related markers than neutral markers because of the selective pressure under heat stress. In addition, population genetic structure analysis showed that neutral markers divided the 432 pea accessions into two subpopulations associated with sowing date type and geographical origin, while the heat-tolerance-related markers divided these germplasms into two subpopulations associated with heat tolerance and sowing date type. Overall, we present a comprehensive resource of heat-tolerant and heat-sensitive pea accessions through heat-tolerance screenings in multi-conditions, which could help genetic improvements of pea in the future.
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spelling pubmed-95736102022-10-17 Large-Scale Heat-Tolerance Screening and Genetic Diversity of Pea (Pisum sativum L.) Germplasms Wang, Dong Yang, Tao Liu, Rong Li, Nana Ahmad, Naveed Li, Guan Ji, Yishan Wang, Chenyu Li, Mengwei Yan, Xin Ding, Hanfeng Zong, Xuxiao Plants (Basel) Article Pea (Pisum sativum L.) is an important legume crop. However, the yield of pea is adversely affected by heat stress in China. In this study, heat-tolerant germplasms were screened and evaluated in the field under multi-conditions. The results showed that heat stress could significantly affect pea yield. On the basis of grain weight per plant, 257 heat-tolerant and 175 heat-sensitive accessions were obtained from the first year’s screening, and 26 extremely heat-tolerant and 19 extremely heat-sensitive accessions were finally obtained in this study. Based on SNaPshot technology, two sets of SNP markers, including 46 neutral and 20 heat-tolerance-related markers, were used to evaluate the genetic diversity and population genetic structure of the 432 pea accessions obtained from the first year’s screening. Genetic diversity analysis showed that the average polymorphic information content was lower using heat-tolerance-related markers than neutral markers because of the selective pressure under heat stress. In addition, population genetic structure analysis showed that neutral markers divided the 432 pea accessions into two subpopulations associated with sowing date type and geographical origin, while the heat-tolerance-related markers divided these germplasms into two subpopulations associated with heat tolerance and sowing date type. Overall, we present a comprehensive resource of heat-tolerant and heat-sensitive pea accessions through heat-tolerance screenings in multi-conditions, which could help genetic improvements of pea in the future. MDPI 2022-09-21 /pmc/articles/PMC9573610/ /pubmed/36235339 http://dx.doi.org/10.3390/plants11192473 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Dong
Yang, Tao
Liu, Rong
Li, Nana
Ahmad, Naveed
Li, Guan
Ji, Yishan
Wang, Chenyu
Li, Mengwei
Yan, Xin
Ding, Hanfeng
Zong, Xuxiao
Large-Scale Heat-Tolerance Screening and Genetic Diversity of Pea (Pisum sativum L.) Germplasms
title Large-Scale Heat-Tolerance Screening and Genetic Diversity of Pea (Pisum sativum L.) Germplasms
title_full Large-Scale Heat-Tolerance Screening and Genetic Diversity of Pea (Pisum sativum L.) Germplasms
title_fullStr Large-Scale Heat-Tolerance Screening and Genetic Diversity of Pea (Pisum sativum L.) Germplasms
title_full_unstemmed Large-Scale Heat-Tolerance Screening and Genetic Diversity of Pea (Pisum sativum L.) Germplasms
title_short Large-Scale Heat-Tolerance Screening and Genetic Diversity of Pea (Pisum sativum L.) Germplasms
title_sort large-scale heat-tolerance screening and genetic diversity of pea (pisum sativum l.) germplasms
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9573610/
https://www.ncbi.nlm.nih.gov/pubmed/36235339
http://dx.doi.org/10.3390/plants11192473
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