Cargando…

Molecular Characterization of Bacterial Isolates from Soil Samples and Evaluation of their Antibacterial Potential against MDRS

Some soil microbes, with their diverse inhabitance, biologically active metabolites, and endospore formation, gave them characteristic predominance and recognition among other microbial communities. The present study collected ten soil samples from green land, agricultural and marshy soil sites of K...

Descripción completa

Detalles Bibliográficos
Autores principales: Sadiqi, Shahida, Hamza, Muhammad, Ali, Farooq, Alam, Sadia, Shakeela, Qismat, Ahmed, Shehzad, Ayaz, Asma, Ali, Sajid, Saqib, Saddam, Ullah, Fazal, Zaman, Wajid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9573682/
https://www.ncbi.nlm.nih.gov/pubmed/36234819
http://dx.doi.org/10.3390/molecules27196281
_version_ 1784810933848113152
author Sadiqi, Shahida
Hamza, Muhammad
Ali, Farooq
Alam, Sadia
Shakeela, Qismat
Ahmed, Shehzad
Ayaz, Asma
Ali, Sajid
Saqib, Saddam
Ullah, Fazal
Zaman, Wajid
author_facet Sadiqi, Shahida
Hamza, Muhammad
Ali, Farooq
Alam, Sadia
Shakeela, Qismat
Ahmed, Shehzad
Ayaz, Asma
Ali, Sajid
Saqib, Saddam
Ullah, Fazal
Zaman, Wajid
author_sort Sadiqi, Shahida
collection PubMed
description Some soil microbes, with their diverse inhabitance, biologically active metabolites, and endospore formation, gave them characteristic predominance and recognition among other microbial communities. The present study collected ten soil samples from green land, agricultural and marshy soil sites of Khyber Pakhtunkhwa, Pakistan. After culturing on described media, the bacterial isolates were identified through phenotypic, biochemical and phylogenetic analysis. Our phylogenetic analysis revealed three bacterial isolates, A6S7, A1S6, and A1S10, showing 99% nucleotides sequence similarity with Brevibacillus formosus, Bacillus Subtilis and Paenibacillus dendritiformis. The crude extract was prepared from bacterial isolates to assess the anti-bacterial potential against various targeted multidrug-resistant strains (MDRS), including Acinetobacter baumannii (ATCC 19606), Methicillin-resistant Staphylococcus aureus (MRSA) (BAA-1683), Klebsiella pneumoniae (ATCC 13883), Pseudomonas aeruginosa (BAA-2108), Staphylococcus aureus (ATCC 292013), Escherichia coli (ATCC25922) and Salmonella typhi (ATCC 14028). Our analysis revealed that all bacterial extracts possess activity against Gram-negative and Gram-positive bacteria at a concentration of 5 mg/mL, efficiently restricting the growth of E. coli compared with positive control ciprofloxacin. The study concluded that the identified species have the potential to produce antimicrobial compounds which can be used to control different microbial infections, especially MDRS. Moreover, the analysis of the bacterial extracts through GC-MS indicated the presence of different antimicrobial compounds such as propanoic acid, oxalic acid, phenol and hexadecanoic acid.
format Online
Article
Text
id pubmed-9573682
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-95736822022-10-17 Molecular Characterization of Bacterial Isolates from Soil Samples and Evaluation of their Antibacterial Potential against MDRS Sadiqi, Shahida Hamza, Muhammad Ali, Farooq Alam, Sadia Shakeela, Qismat Ahmed, Shehzad Ayaz, Asma Ali, Sajid Saqib, Saddam Ullah, Fazal Zaman, Wajid Molecules Article Some soil microbes, with their diverse inhabitance, biologically active metabolites, and endospore formation, gave them characteristic predominance and recognition among other microbial communities. The present study collected ten soil samples from green land, agricultural and marshy soil sites of Khyber Pakhtunkhwa, Pakistan. After culturing on described media, the bacterial isolates were identified through phenotypic, biochemical and phylogenetic analysis. Our phylogenetic analysis revealed three bacterial isolates, A6S7, A1S6, and A1S10, showing 99% nucleotides sequence similarity with Brevibacillus formosus, Bacillus Subtilis and Paenibacillus dendritiformis. The crude extract was prepared from bacterial isolates to assess the anti-bacterial potential against various targeted multidrug-resistant strains (MDRS), including Acinetobacter baumannii (ATCC 19606), Methicillin-resistant Staphylococcus aureus (MRSA) (BAA-1683), Klebsiella pneumoniae (ATCC 13883), Pseudomonas aeruginosa (BAA-2108), Staphylococcus aureus (ATCC 292013), Escherichia coli (ATCC25922) and Salmonella typhi (ATCC 14028). Our analysis revealed that all bacterial extracts possess activity against Gram-negative and Gram-positive bacteria at a concentration of 5 mg/mL, efficiently restricting the growth of E. coli compared with positive control ciprofloxacin. The study concluded that the identified species have the potential to produce antimicrobial compounds which can be used to control different microbial infections, especially MDRS. Moreover, the analysis of the bacterial extracts through GC-MS indicated the presence of different antimicrobial compounds such as propanoic acid, oxalic acid, phenol and hexadecanoic acid. MDPI 2022-09-23 /pmc/articles/PMC9573682/ /pubmed/36234819 http://dx.doi.org/10.3390/molecules27196281 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sadiqi, Shahida
Hamza, Muhammad
Ali, Farooq
Alam, Sadia
Shakeela, Qismat
Ahmed, Shehzad
Ayaz, Asma
Ali, Sajid
Saqib, Saddam
Ullah, Fazal
Zaman, Wajid
Molecular Characterization of Bacterial Isolates from Soil Samples and Evaluation of their Antibacterial Potential against MDRS
title Molecular Characterization of Bacterial Isolates from Soil Samples and Evaluation of their Antibacterial Potential against MDRS
title_full Molecular Characterization of Bacterial Isolates from Soil Samples and Evaluation of their Antibacterial Potential against MDRS
title_fullStr Molecular Characterization of Bacterial Isolates from Soil Samples and Evaluation of their Antibacterial Potential against MDRS
title_full_unstemmed Molecular Characterization of Bacterial Isolates from Soil Samples and Evaluation of their Antibacterial Potential against MDRS
title_short Molecular Characterization of Bacterial Isolates from Soil Samples and Evaluation of their Antibacterial Potential against MDRS
title_sort molecular characterization of bacterial isolates from soil samples and evaluation of their antibacterial potential against mdrs
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9573682/
https://www.ncbi.nlm.nih.gov/pubmed/36234819
http://dx.doi.org/10.3390/molecules27196281
work_keys_str_mv AT sadiqishahida molecularcharacterizationofbacterialisolatesfromsoilsamplesandevaluationoftheirantibacterialpotentialagainstmdrs
AT hamzamuhammad molecularcharacterizationofbacterialisolatesfromsoilsamplesandevaluationoftheirantibacterialpotentialagainstmdrs
AT alifarooq molecularcharacterizationofbacterialisolatesfromsoilsamplesandevaluationoftheirantibacterialpotentialagainstmdrs
AT alamsadia molecularcharacterizationofbacterialisolatesfromsoilsamplesandevaluationoftheirantibacterialpotentialagainstmdrs
AT shakeelaqismat molecularcharacterizationofbacterialisolatesfromsoilsamplesandevaluationoftheirantibacterialpotentialagainstmdrs
AT ahmedshehzad molecularcharacterizationofbacterialisolatesfromsoilsamplesandevaluationoftheirantibacterialpotentialagainstmdrs
AT ayazasma molecularcharacterizationofbacterialisolatesfromsoilsamplesandevaluationoftheirantibacterialpotentialagainstmdrs
AT alisajid molecularcharacterizationofbacterialisolatesfromsoilsamplesandevaluationoftheirantibacterialpotentialagainstmdrs
AT saqibsaddam molecularcharacterizationofbacterialisolatesfromsoilsamplesandevaluationoftheirantibacterialpotentialagainstmdrs
AT ullahfazal molecularcharacterizationofbacterialisolatesfromsoilsamplesandevaluationoftheirantibacterialpotentialagainstmdrs
AT zamanwajid molecularcharacterizationofbacterialisolatesfromsoilsamplesandevaluationoftheirantibacterialpotentialagainstmdrs