Cargando…
Comparison of vaccine-induced antibody neutralization against SARS-CoV-2 variants of concern following primary and booster doses of COVID-19 vaccines
The SARS-CoV-2 pandemic has, as of July 2022, infected more than 550 million people and caused over 6 million deaths across the world. COVID-19 vaccines were quickly developed to protect against severe disease, hospitalization and death. In the present study, we performed a direct comparative analys...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9574042/ https://www.ncbi.nlm.nih.gov/pubmed/36262278 http://dx.doi.org/10.3389/fmed.2022.994160 |
_version_ | 1784811015171473408 |
---|---|
author | Hvidt, Astrid K. Baerends, Eva A. M. Søgaard, Ole S. Stærke, Nina B. Raben, Dorthe Reekie, Joanne Nielsen, Henrik Johansen, Isik S. Wiese, Lothar Benfield, Thomas L. Iversen, Kasper K. Mustafa, Ahmed B. Juhl, Maria R. Petersen, Kristine T. Ostrowski, Sisse R. Lindvig, Susan O. Rasmussen, Line D. Schleimann, Marianne H. Andersen, Sidsel D. Juhl, Anna K. Dietz, Lisa L. Andreasen, Signe R. Lundgren, Jens Østergaard, Lars Tolstrup, Martin |
author_facet | Hvidt, Astrid K. Baerends, Eva A. M. Søgaard, Ole S. Stærke, Nina B. Raben, Dorthe Reekie, Joanne Nielsen, Henrik Johansen, Isik S. Wiese, Lothar Benfield, Thomas L. Iversen, Kasper K. Mustafa, Ahmed B. Juhl, Maria R. Petersen, Kristine T. Ostrowski, Sisse R. Lindvig, Susan O. Rasmussen, Line D. Schleimann, Marianne H. Andersen, Sidsel D. Juhl, Anna K. Dietz, Lisa L. Andreasen, Signe R. Lundgren, Jens Østergaard, Lars Tolstrup, Martin |
author_sort | Hvidt, Astrid K. |
collection | PubMed |
description | The SARS-CoV-2 pandemic has, as of July 2022, infected more than 550 million people and caused over 6 million deaths across the world. COVID-19 vaccines were quickly developed to protect against severe disease, hospitalization and death. In the present study, we performed a direct comparative analysis of four COVID-19 vaccines: BNT162b2 (Pfizer/BioNTech), mRNA-1273 (Moderna), ChAdOx1 (Oxford/AstraZeneca) and Ad26.COV2.S (Johnson & Johnson/Janssen), following primary and booster vaccination. We focused on the vaccine-induced antibody-mediated immune response against multiple SARS-CoV-2 variants: wildtype, B.1.1.7 (Alpha), B.1.351 (Beta), B.1.617.2 (Delta) and B.1.1.529 (Omicron). The analysis included the quantification of total IgG levels against SARS-CoV-2 Spike, as well as the quantification of antibody neutralization titers. Furthermore, the study assessed the high-throughput ACE2 competition assay as a surrogate for the traditional pseudovirus neutralization assay. The results demonstrated marked differences in antibody-mediated immune responses. The lowest Spike-specific IgG levels and antibody neutralization titers were induced by one dose of the Ad26.COV2.S vaccine, intermediate levels by two doses of the BNT162b2 vaccine, and the highest levels by two doses of the mRNA-1273 vaccine or heterologous vaccination of one dose of the ChAdOx1 vaccine and a subsequent mRNA vaccine. The study also demonstrated that accumulation of SARS-CoV-2 Spike protein mutations was accompanied by a marked decline in antibody neutralization capacity, especially for B.1.1.529. Administration of a booster dose was shown to significantly increase Spike-specific IgG levels and antibody neutralization titers, erasing the differences between the vaccine-induced antibody-mediated immune response between the four vaccines. The findings of this study highlight the importance of booster vaccines and the potential inclusion of future heterologous vaccination strategies for broad protection against current and emerging SARS-CoV-2 variants. |
format | Online Article Text |
id | pubmed-9574042 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-95740422022-10-18 Comparison of vaccine-induced antibody neutralization against SARS-CoV-2 variants of concern following primary and booster doses of COVID-19 vaccines Hvidt, Astrid K. Baerends, Eva A. M. Søgaard, Ole S. Stærke, Nina B. Raben, Dorthe Reekie, Joanne Nielsen, Henrik Johansen, Isik S. Wiese, Lothar Benfield, Thomas L. Iversen, Kasper K. Mustafa, Ahmed B. Juhl, Maria R. Petersen, Kristine T. Ostrowski, Sisse R. Lindvig, Susan O. Rasmussen, Line D. Schleimann, Marianne H. Andersen, Sidsel D. Juhl, Anna K. Dietz, Lisa L. Andreasen, Signe R. Lundgren, Jens Østergaard, Lars Tolstrup, Martin Front Med (Lausanne) Medicine The SARS-CoV-2 pandemic has, as of July 2022, infected more than 550 million people and caused over 6 million deaths across the world. COVID-19 vaccines were quickly developed to protect against severe disease, hospitalization and death. In the present study, we performed a direct comparative analysis of four COVID-19 vaccines: BNT162b2 (Pfizer/BioNTech), mRNA-1273 (Moderna), ChAdOx1 (Oxford/AstraZeneca) and Ad26.COV2.S (Johnson & Johnson/Janssen), following primary and booster vaccination. We focused on the vaccine-induced antibody-mediated immune response against multiple SARS-CoV-2 variants: wildtype, B.1.1.7 (Alpha), B.1.351 (Beta), B.1.617.2 (Delta) and B.1.1.529 (Omicron). The analysis included the quantification of total IgG levels against SARS-CoV-2 Spike, as well as the quantification of antibody neutralization titers. Furthermore, the study assessed the high-throughput ACE2 competition assay as a surrogate for the traditional pseudovirus neutralization assay. The results demonstrated marked differences in antibody-mediated immune responses. The lowest Spike-specific IgG levels and antibody neutralization titers were induced by one dose of the Ad26.COV2.S vaccine, intermediate levels by two doses of the BNT162b2 vaccine, and the highest levels by two doses of the mRNA-1273 vaccine or heterologous vaccination of one dose of the ChAdOx1 vaccine and a subsequent mRNA vaccine. The study also demonstrated that accumulation of SARS-CoV-2 Spike protein mutations was accompanied by a marked decline in antibody neutralization capacity, especially for B.1.1.529. Administration of a booster dose was shown to significantly increase Spike-specific IgG levels and antibody neutralization titers, erasing the differences between the vaccine-induced antibody-mediated immune response between the four vaccines. The findings of this study highlight the importance of booster vaccines and the potential inclusion of future heterologous vaccination strategies for broad protection against current and emerging SARS-CoV-2 variants. Frontiers Media S.A. 2022-10-03 /pmc/articles/PMC9574042/ /pubmed/36262278 http://dx.doi.org/10.3389/fmed.2022.994160 Text en Copyright © 2022 Hvidt, Baerends, Søgaard, Stærke, Raben, Reekie, Nielsen, Johansen, Wiese, Benfield, Iversen, Mustafa, Juhl, Petersen, Ostrowski, Lindvig, Rasmussen, Schleimann, Andersen, Juhl, Dietz, Andreasen, Lundgren, Østergaard, Tolstrup and the ENFORCE Study Group. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Medicine Hvidt, Astrid K. Baerends, Eva A. M. Søgaard, Ole S. Stærke, Nina B. Raben, Dorthe Reekie, Joanne Nielsen, Henrik Johansen, Isik S. Wiese, Lothar Benfield, Thomas L. Iversen, Kasper K. Mustafa, Ahmed B. Juhl, Maria R. Petersen, Kristine T. Ostrowski, Sisse R. Lindvig, Susan O. Rasmussen, Line D. Schleimann, Marianne H. Andersen, Sidsel D. Juhl, Anna K. Dietz, Lisa L. Andreasen, Signe R. Lundgren, Jens Østergaard, Lars Tolstrup, Martin Comparison of vaccine-induced antibody neutralization against SARS-CoV-2 variants of concern following primary and booster doses of COVID-19 vaccines |
title | Comparison of vaccine-induced antibody neutralization against SARS-CoV-2 variants of concern following primary and booster doses of COVID-19 vaccines |
title_full | Comparison of vaccine-induced antibody neutralization against SARS-CoV-2 variants of concern following primary and booster doses of COVID-19 vaccines |
title_fullStr | Comparison of vaccine-induced antibody neutralization against SARS-CoV-2 variants of concern following primary and booster doses of COVID-19 vaccines |
title_full_unstemmed | Comparison of vaccine-induced antibody neutralization against SARS-CoV-2 variants of concern following primary and booster doses of COVID-19 vaccines |
title_short | Comparison of vaccine-induced antibody neutralization against SARS-CoV-2 variants of concern following primary and booster doses of COVID-19 vaccines |
title_sort | comparison of vaccine-induced antibody neutralization against sars-cov-2 variants of concern following primary and booster doses of covid-19 vaccines |
topic | Medicine |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9574042/ https://www.ncbi.nlm.nih.gov/pubmed/36262278 http://dx.doi.org/10.3389/fmed.2022.994160 |
work_keys_str_mv | AT hvidtastridk comparisonofvaccineinducedantibodyneutralizationagainstsarscov2variantsofconcernfollowingprimaryandboosterdosesofcovid19vaccines AT baerendsevaam comparisonofvaccineinducedantibodyneutralizationagainstsarscov2variantsofconcernfollowingprimaryandboosterdosesofcovid19vaccines AT søgaardoles comparisonofvaccineinducedantibodyneutralizationagainstsarscov2variantsofconcernfollowingprimaryandboosterdosesofcovid19vaccines AT stærkeninab comparisonofvaccineinducedantibodyneutralizationagainstsarscov2variantsofconcernfollowingprimaryandboosterdosesofcovid19vaccines AT rabendorthe comparisonofvaccineinducedantibodyneutralizationagainstsarscov2variantsofconcernfollowingprimaryandboosterdosesofcovid19vaccines AT reekiejoanne comparisonofvaccineinducedantibodyneutralizationagainstsarscov2variantsofconcernfollowingprimaryandboosterdosesofcovid19vaccines AT nielsenhenrik comparisonofvaccineinducedantibodyneutralizationagainstsarscov2variantsofconcernfollowingprimaryandboosterdosesofcovid19vaccines AT johansenisiks comparisonofvaccineinducedantibodyneutralizationagainstsarscov2variantsofconcernfollowingprimaryandboosterdosesofcovid19vaccines AT wieselothar comparisonofvaccineinducedantibodyneutralizationagainstsarscov2variantsofconcernfollowingprimaryandboosterdosesofcovid19vaccines AT benfieldthomasl comparisonofvaccineinducedantibodyneutralizationagainstsarscov2variantsofconcernfollowingprimaryandboosterdosesofcovid19vaccines AT iversenkasperk comparisonofvaccineinducedantibodyneutralizationagainstsarscov2variantsofconcernfollowingprimaryandboosterdosesofcovid19vaccines AT mustafaahmedb comparisonofvaccineinducedantibodyneutralizationagainstsarscov2variantsofconcernfollowingprimaryandboosterdosesofcovid19vaccines AT juhlmariar comparisonofvaccineinducedantibodyneutralizationagainstsarscov2variantsofconcernfollowingprimaryandboosterdosesofcovid19vaccines AT petersenkristinet comparisonofvaccineinducedantibodyneutralizationagainstsarscov2variantsofconcernfollowingprimaryandboosterdosesofcovid19vaccines AT ostrowskisisser comparisonofvaccineinducedantibodyneutralizationagainstsarscov2variantsofconcernfollowingprimaryandboosterdosesofcovid19vaccines AT lindvigsusano comparisonofvaccineinducedantibodyneutralizationagainstsarscov2variantsofconcernfollowingprimaryandboosterdosesofcovid19vaccines AT rasmussenlined comparisonofvaccineinducedantibodyneutralizationagainstsarscov2variantsofconcernfollowingprimaryandboosterdosesofcovid19vaccines AT schleimannmarianneh comparisonofvaccineinducedantibodyneutralizationagainstsarscov2variantsofconcernfollowingprimaryandboosterdosesofcovid19vaccines AT andersensidseld comparisonofvaccineinducedantibodyneutralizationagainstsarscov2variantsofconcernfollowingprimaryandboosterdosesofcovid19vaccines AT juhlannak comparisonofvaccineinducedantibodyneutralizationagainstsarscov2variantsofconcernfollowingprimaryandboosterdosesofcovid19vaccines AT dietzlisal comparisonofvaccineinducedantibodyneutralizationagainstsarscov2variantsofconcernfollowingprimaryandboosterdosesofcovid19vaccines AT andreasensigner comparisonofvaccineinducedantibodyneutralizationagainstsarscov2variantsofconcernfollowingprimaryandboosterdosesofcovid19vaccines AT lundgrenjens comparisonofvaccineinducedantibodyneutralizationagainstsarscov2variantsofconcernfollowingprimaryandboosterdosesofcovid19vaccines AT østergaardlars comparisonofvaccineinducedantibodyneutralizationagainstsarscov2variantsofconcernfollowingprimaryandboosterdosesofcovid19vaccines AT tolstrupmartin comparisonofvaccineinducedantibodyneutralizationagainstsarscov2variantsofconcernfollowingprimaryandboosterdosesofcovid19vaccines AT comparisonofvaccineinducedantibodyneutralizationagainstsarscov2variantsofconcernfollowingprimaryandboosterdosesofcovid19vaccines |