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A therapeutic chimeric IgG/IgA expressed by CHO cells for oral treatment of PED in piglets

Immunoglobulin A (IgA) of sows is critically important for assessing piglets’ protective capacity against porcine epidemic diarrhea virus (PEDV). Here, we report a therapeutic chimeric anti-PEDV IgG/IgA expressed by Chinese hamster ovary (CHO) cells for oral treatment of PED. The chimeric anti-PEDV...

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Autores principales: Xiao, Yan, Zhang, Yunjing, Wang, Zhiyan, Zhao, Wenyin, Xu, Xin, Chen, Xiao, Tan, Feifei, Sun, Zhe, Huang, Baicheng, Tian, Kegong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9574063/
https://www.ncbi.nlm.nih.gov/pubmed/36262323
http://dx.doi.org/10.3389/fmicb.2022.1018748
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author Xiao, Yan
Zhang, Yunjing
Wang, Zhiyan
Zhao, Wenyin
Xu, Xin
Chen, Xiao
Tan, Feifei
Sun, Zhe
Huang, Baicheng
Tian, Kegong
author_facet Xiao, Yan
Zhang, Yunjing
Wang, Zhiyan
Zhao, Wenyin
Xu, Xin
Chen, Xiao
Tan, Feifei
Sun, Zhe
Huang, Baicheng
Tian, Kegong
author_sort Xiao, Yan
collection PubMed
description Immunoglobulin A (IgA) of sows is critically important for assessing piglets’ protective capacity against porcine epidemic diarrhea virus (PEDV). Here, we report a therapeutic chimeric anti-PEDV IgG/IgA expressed by Chinese hamster ovary (CHO) cells for oral treatment of PED. The chimeric anti-PEDV IgG/IgA was produced by the CHO cell lines, in which the heavy chain was constructed by combining the VH, Cγ1 and hinge regions of PEDV IgG mAb 8A3, and the Cα2 and Cα3 domains of a Mus musculus immunoglobulin alpha chain. The chimeric anti-PEDV IgG/IgA could neutralize the strains of CV777 (G1), P014 (G2) and HN1303 (G2) in vitro effectively, showing broad-spectrum neutralization activity. The in vivo challenge experiments demonstrated that chimeric anti-PEDV IgG/IgA (9C4) produced in the CHO cell supernatant could alleviate clinical diarrhea symptoms of the PEDV infection in piglets. In general, our study showed that chimeric anti-PEDV IgG/IgA produced from CHO cell line supernatants effectively alleviates PEDV infection in piglets, which also gives the foundation for the construction of fully functional secretory IgA by the J chain introduction to maximize the antibody therapeutic effect.
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spelling pubmed-95740632022-10-18 A therapeutic chimeric IgG/IgA expressed by CHO cells for oral treatment of PED in piglets Xiao, Yan Zhang, Yunjing Wang, Zhiyan Zhao, Wenyin Xu, Xin Chen, Xiao Tan, Feifei Sun, Zhe Huang, Baicheng Tian, Kegong Front Microbiol Microbiology Immunoglobulin A (IgA) of sows is critically important for assessing piglets’ protective capacity against porcine epidemic diarrhea virus (PEDV). Here, we report a therapeutic chimeric anti-PEDV IgG/IgA expressed by Chinese hamster ovary (CHO) cells for oral treatment of PED. The chimeric anti-PEDV IgG/IgA was produced by the CHO cell lines, in which the heavy chain was constructed by combining the VH, Cγ1 and hinge regions of PEDV IgG mAb 8A3, and the Cα2 and Cα3 domains of a Mus musculus immunoglobulin alpha chain. The chimeric anti-PEDV IgG/IgA could neutralize the strains of CV777 (G1), P014 (G2) and HN1303 (G2) in vitro effectively, showing broad-spectrum neutralization activity. The in vivo challenge experiments demonstrated that chimeric anti-PEDV IgG/IgA (9C4) produced in the CHO cell supernatant could alleviate clinical diarrhea symptoms of the PEDV infection in piglets. In general, our study showed that chimeric anti-PEDV IgG/IgA produced from CHO cell line supernatants effectively alleviates PEDV infection in piglets, which also gives the foundation for the construction of fully functional secretory IgA by the J chain introduction to maximize the antibody therapeutic effect. Frontiers Media S.A. 2022-10-03 /pmc/articles/PMC9574063/ /pubmed/36262323 http://dx.doi.org/10.3389/fmicb.2022.1018748 Text en Copyright © 2022 Xiao, Zhang, Wang, Zhao, Xu, Chen, Tan, Sun, Huang and Tian. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Xiao, Yan
Zhang, Yunjing
Wang, Zhiyan
Zhao, Wenyin
Xu, Xin
Chen, Xiao
Tan, Feifei
Sun, Zhe
Huang, Baicheng
Tian, Kegong
A therapeutic chimeric IgG/IgA expressed by CHO cells for oral treatment of PED in piglets
title A therapeutic chimeric IgG/IgA expressed by CHO cells for oral treatment of PED in piglets
title_full A therapeutic chimeric IgG/IgA expressed by CHO cells for oral treatment of PED in piglets
title_fullStr A therapeutic chimeric IgG/IgA expressed by CHO cells for oral treatment of PED in piglets
title_full_unstemmed A therapeutic chimeric IgG/IgA expressed by CHO cells for oral treatment of PED in piglets
title_short A therapeutic chimeric IgG/IgA expressed by CHO cells for oral treatment of PED in piglets
title_sort therapeutic chimeric igg/iga expressed by cho cells for oral treatment of ped in piglets
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9574063/
https://www.ncbi.nlm.nih.gov/pubmed/36262323
http://dx.doi.org/10.3389/fmicb.2022.1018748
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