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Anti-biofilm efficacy of green-synthesized ZnO nanoparticles on oral biofilm: In vitro and in silico study

The development of biofilm on the biotic and abiotic surfaces is the greatest challenge for health care sectors. At present times, oral infection is a common concern among people with an unhealthy lifestyle and most of these biofilms-associated infections are resistant to antibiotics. This has incre...

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Autores principales: Lahiri, Dibyajit, Ray, Rina Rani, Sarkar, Tanmay, Upadhye, Vijay Jagdish, Ghosh, Sujay, Pandit, Soumya, Pati, Siddhartha, Edinur, Hisham Atan, Abdul Kari, Zulhisyam, Nag, Moupriya, Ahmad Mohd Zain, Muhammad Rajaei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9574224/
https://www.ncbi.nlm.nih.gov/pubmed/36262331
http://dx.doi.org/10.3389/fmicb.2022.939390
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author Lahiri, Dibyajit
Ray, Rina Rani
Sarkar, Tanmay
Upadhye, Vijay Jagdish
Ghosh, Sujay
Pandit, Soumya
Pati, Siddhartha
Edinur, Hisham Atan
Abdul Kari, Zulhisyam
Nag, Moupriya
Ahmad Mohd Zain, Muhammad Rajaei
author_facet Lahiri, Dibyajit
Ray, Rina Rani
Sarkar, Tanmay
Upadhye, Vijay Jagdish
Ghosh, Sujay
Pandit, Soumya
Pati, Siddhartha
Edinur, Hisham Atan
Abdul Kari, Zulhisyam
Nag, Moupriya
Ahmad Mohd Zain, Muhammad Rajaei
author_sort Lahiri, Dibyajit
collection PubMed
description The development of biofilm on the biotic and abiotic surfaces is the greatest challenge for health care sectors. At present times, oral infection is a common concern among people with an unhealthy lifestyle and most of these biofilms-associated infections are resistant to antibiotics. This has increased a search for the development of alternate therapeutics for eradicating biofilm-associated infection. Nanobiotechnology being an effective way to combat such oral infections may encourage the use of herbal compounds, such as bio-reducing and capping agents. Green-synthesis of ZnO nanoparticles (ZnO NP) by the use of the floral extract of Clitoria ternatea, a traditionally used medicinal plant, showed stability for a longer period of time. The NPs as depicted by the TEM image with a size of 10 nm showed excitation spectra at 360 nm and were found to remain stable for a considerable period of time. It was observed that the NPs were effective in the eradication of the oral biofilm formed by the major tooth attacking bacterial strains namely Porphyromonsas gingivalis and Alcaligenes faecalis, by bringing a considerable reduction in the extracellular polymeric substances (EPS). It was observed that the viability of the Porphyromonsas gingivalis and Alcaligenes faecalis was reduced by NP treatment to 87.89 ± 0.25% in comparison to that of amoxicillin. The results went in agreement with the findings of modeling performed by the use of response surface methodology (RSM) and artificial neural network (ANN). The microscopic studies and FT-IR analysis revealed that there was a considerable reduction in the biofilm after NP treatment. The in silico studies further confirmed that the ZnO NPs showed considerable interactions with the biofilm-forming proteins. Hence, this study showed that ZnO NPs derived from Clitoria ternatea can be used as an effective alternative therapeutic for the treatment of biofilm associated oral infection.
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spelling pubmed-95742242022-10-18 Anti-biofilm efficacy of green-synthesized ZnO nanoparticles on oral biofilm: In vitro and in silico study Lahiri, Dibyajit Ray, Rina Rani Sarkar, Tanmay Upadhye, Vijay Jagdish Ghosh, Sujay Pandit, Soumya Pati, Siddhartha Edinur, Hisham Atan Abdul Kari, Zulhisyam Nag, Moupriya Ahmad Mohd Zain, Muhammad Rajaei Front Microbiol Microbiology The development of biofilm on the biotic and abiotic surfaces is the greatest challenge for health care sectors. At present times, oral infection is a common concern among people with an unhealthy lifestyle and most of these biofilms-associated infections are resistant to antibiotics. This has increased a search for the development of alternate therapeutics for eradicating biofilm-associated infection. Nanobiotechnology being an effective way to combat such oral infections may encourage the use of herbal compounds, such as bio-reducing and capping agents. Green-synthesis of ZnO nanoparticles (ZnO NP) by the use of the floral extract of Clitoria ternatea, a traditionally used medicinal plant, showed stability for a longer period of time. The NPs as depicted by the TEM image with a size of 10 nm showed excitation spectra at 360 nm and were found to remain stable for a considerable period of time. It was observed that the NPs were effective in the eradication of the oral biofilm formed by the major tooth attacking bacterial strains namely Porphyromonsas gingivalis and Alcaligenes faecalis, by bringing a considerable reduction in the extracellular polymeric substances (EPS). It was observed that the viability of the Porphyromonsas gingivalis and Alcaligenes faecalis was reduced by NP treatment to 87.89 ± 0.25% in comparison to that of amoxicillin. The results went in agreement with the findings of modeling performed by the use of response surface methodology (RSM) and artificial neural network (ANN). The microscopic studies and FT-IR analysis revealed that there was a considerable reduction in the biofilm after NP treatment. The in silico studies further confirmed that the ZnO NPs showed considerable interactions with the biofilm-forming proteins. Hence, this study showed that ZnO NPs derived from Clitoria ternatea can be used as an effective alternative therapeutic for the treatment of biofilm associated oral infection. Frontiers Media S.A. 2022-10-03 /pmc/articles/PMC9574224/ /pubmed/36262331 http://dx.doi.org/10.3389/fmicb.2022.939390 Text en Copyright © 2022 Lahiri, Ray, Sarkar, Upadhye, Ghosh, Pandit, Pati, Edinur, Abdul Kari, Nag and Ahmad Mohd Zain. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Lahiri, Dibyajit
Ray, Rina Rani
Sarkar, Tanmay
Upadhye, Vijay Jagdish
Ghosh, Sujay
Pandit, Soumya
Pati, Siddhartha
Edinur, Hisham Atan
Abdul Kari, Zulhisyam
Nag, Moupriya
Ahmad Mohd Zain, Muhammad Rajaei
Anti-biofilm efficacy of green-synthesized ZnO nanoparticles on oral biofilm: In vitro and in silico study
title Anti-biofilm efficacy of green-synthesized ZnO nanoparticles on oral biofilm: In vitro and in silico study
title_full Anti-biofilm efficacy of green-synthesized ZnO nanoparticles on oral biofilm: In vitro and in silico study
title_fullStr Anti-biofilm efficacy of green-synthesized ZnO nanoparticles on oral biofilm: In vitro and in silico study
title_full_unstemmed Anti-biofilm efficacy of green-synthesized ZnO nanoparticles on oral biofilm: In vitro and in silico study
title_short Anti-biofilm efficacy of green-synthesized ZnO nanoparticles on oral biofilm: In vitro and in silico study
title_sort anti-biofilm efficacy of green-synthesized zno nanoparticles on oral biofilm: in vitro and in silico study
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9574224/
https://www.ncbi.nlm.nih.gov/pubmed/36262331
http://dx.doi.org/10.3389/fmicb.2022.939390
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