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Effect of serotonin on the cell viability of the bovine mammary alveolar cell-T (MAC-T) cell line

5-Hydroxytryptamine (5-HT), a monoamine, as a local regulator in the mammary gland is a chemical signal produced by the mammary epithelium cell. In cows, studies have shown that 5-HT is associated with epithelial cell apoptosis during the degenerative phase of the mammary gland. However, studies in...

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Autores principales: Dong, Xusheng, Liu, Chen, Miao, Jialin, Lin, Xueyan, Wang, Yun, Wang, Zhonghua, Hou, Qiuling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society of Animal Sciences and Technology 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9574616/
https://www.ncbi.nlm.nih.gov/pubmed/36287778
http://dx.doi.org/10.5187/jast.2022.e50
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author Dong, Xusheng
Liu, Chen
Miao, Jialin
Lin, Xueyan
Wang, Yun
Wang, Zhonghua
Hou, Qiuling
author_facet Dong, Xusheng
Liu, Chen
Miao, Jialin
Lin, Xueyan
Wang, Yun
Wang, Zhonghua
Hou, Qiuling
author_sort Dong, Xusheng
collection PubMed
description 5-Hydroxytryptamine (5-HT), a monoamine, as a local regulator in the mammary gland is a chemical signal produced by the mammary epithelium cell. In cows, studies have shown that 5-HT is associated with epithelial cell apoptosis during the degenerative phase of the mammary gland. However, studies in other tissues have shown that 5-HT can effectively promote cell viability. Whether 5-HT could have an effect on mammary cell viability in dairy cows is still unknown. The purpose of this study was to determine: (1) effect of 5-HT on the viability of bovine mammary epithelial cells and its related signaling pathways, (2) interaction between prolactin (PRL) and 5-HT on the cell viability. The bovine mammary alveolar cell-T (MAC-T) were cultured with different concentrations of 5-HT for 12, 24, 48 or 72 hours, and then were assayed using cell counting kit-8, polymerase chain reaction (PCR) and immunobloting. The results suggested that 20 μM 5-HT treatment for 12 or 24 h promote cell viability, which was mainly induced by the activation of 5-HT receptor (5-HTR) 1B and 4, because the increase caused by 5-HT vanished when 5-HTR 1B and 4 was blocked by SB224289 and SB204070. And protein expression of mammalian target of rapamycin (mTOR), eukaryotic translation elongation factor 2 (eEF2), janus kinase 2 (JAK2) and signal transducer and activator of transcription 5 (STAT5) were decreased after blocking 5-HT 1B and 4 receptors. When MAC-T cells were treated with 5-HT and PRL simultaneously for 24 h, both the cell viability and the level of mTOR protein were significantly higher than that cultured with 5-HT or PRL alone. In conclusion, our study suggested that 5-HT promotes the viability of MAC-T cells by 5-HTR 1B and/or 4. Furthermore, there is a reciprocal relationship between PRL and 5-HT.
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spelling pubmed-95746162022-10-24 Effect of serotonin on the cell viability of the bovine mammary alveolar cell-T (MAC-T) cell line Dong, Xusheng Liu, Chen Miao, Jialin Lin, Xueyan Wang, Yun Wang, Zhonghua Hou, Qiuling J Anim Sci Technol Research Article 5-Hydroxytryptamine (5-HT), a monoamine, as a local regulator in the mammary gland is a chemical signal produced by the mammary epithelium cell. In cows, studies have shown that 5-HT is associated with epithelial cell apoptosis during the degenerative phase of the mammary gland. However, studies in other tissues have shown that 5-HT can effectively promote cell viability. Whether 5-HT could have an effect on mammary cell viability in dairy cows is still unknown. The purpose of this study was to determine: (1) effect of 5-HT on the viability of bovine mammary epithelial cells and its related signaling pathways, (2) interaction between prolactin (PRL) and 5-HT on the cell viability. The bovine mammary alveolar cell-T (MAC-T) were cultured with different concentrations of 5-HT for 12, 24, 48 or 72 hours, and then were assayed using cell counting kit-8, polymerase chain reaction (PCR) and immunobloting. The results suggested that 20 μM 5-HT treatment for 12 or 24 h promote cell viability, which was mainly induced by the activation of 5-HT receptor (5-HTR) 1B and 4, because the increase caused by 5-HT vanished when 5-HTR 1B and 4 was blocked by SB224289 and SB204070. And protein expression of mammalian target of rapamycin (mTOR), eukaryotic translation elongation factor 2 (eEF2), janus kinase 2 (JAK2) and signal transducer and activator of transcription 5 (STAT5) were decreased after blocking 5-HT 1B and 4 receptors. When MAC-T cells were treated with 5-HT and PRL simultaneously for 24 h, both the cell viability and the level of mTOR protein were significantly higher than that cultured with 5-HT or PRL alone. In conclusion, our study suggested that 5-HT promotes the viability of MAC-T cells by 5-HTR 1B and/or 4. Furthermore, there is a reciprocal relationship between PRL and 5-HT. Korean Society of Animal Sciences and Technology 2022-09 2022-09-30 /pmc/articles/PMC9574616/ /pubmed/36287778 http://dx.doi.org/10.5187/jast.2022.e50 Text en © Copyright 2022 Korean Society of Animal Science and Technology https://creativecommons.org/licenses/by-nc/4.0/This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Dong, Xusheng
Liu, Chen
Miao, Jialin
Lin, Xueyan
Wang, Yun
Wang, Zhonghua
Hou, Qiuling
Effect of serotonin on the cell viability of the bovine mammary alveolar cell-T (MAC-T) cell line
title Effect of serotonin on the cell viability of the bovine mammary alveolar cell-T (MAC-T) cell line
title_full Effect of serotonin on the cell viability of the bovine mammary alveolar cell-T (MAC-T) cell line
title_fullStr Effect of serotonin on the cell viability of the bovine mammary alveolar cell-T (MAC-T) cell line
title_full_unstemmed Effect of serotonin on the cell viability of the bovine mammary alveolar cell-T (MAC-T) cell line
title_short Effect of serotonin on the cell viability of the bovine mammary alveolar cell-T (MAC-T) cell line
title_sort effect of serotonin on the cell viability of the bovine mammary alveolar cell-t (mac-t) cell line
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9574616/
https://www.ncbi.nlm.nih.gov/pubmed/36287778
http://dx.doi.org/10.5187/jast.2022.e50
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