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Effect of serotonin on the cell viability of the bovine mammary alveolar cell-T (MAC-T) cell line
5-Hydroxytryptamine (5-HT), a monoamine, as a local regulator in the mammary gland is a chemical signal produced by the mammary epithelium cell. In cows, studies have shown that 5-HT is associated with epithelial cell apoptosis during the degenerative phase of the mammary gland. However, studies in...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Korean Society of Animal Sciences and Technology
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9574616/ https://www.ncbi.nlm.nih.gov/pubmed/36287778 http://dx.doi.org/10.5187/jast.2022.e50 |
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author | Dong, Xusheng Liu, Chen Miao, Jialin Lin, Xueyan Wang, Yun Wang, Zhonghua Hou, Qiuling |
author_facet | Dong, Xusheng Liu, Chen Miao, Jialin Lin, Xueyan Wang, Yun Wang, Zhonghua Hou, Qiuling |
author_sort | Dong, Xusheng |
collection | PubMed |
description | 5-Hydroxytryptamine (5-HT), a monoamine, as a local regulator in the mammary gland is a chemical signal produced by the mammary epithelium cell. In cows, studies have shown that 5-HT is associated with epithelial cell apoptosis during the degenerative phase of the mammary gland. However, studies in other tissues have shown that 5-HT can effectively promote cell viability. Whether 5-HT could have an effect on mammary cell viability in dairy cows is still unknown. The purpose of this study was to determine: (1) effect of 5-HT on the viability of bovine mammary epithelial cells and its related signaling pathways, (2) interaction between prolactin (PRL) and 5-HT on the cell viability. The bovine mammary alveolar cell-T (MAC-T) were cultured with different concentrations of 5-HT for 12, 24, 48 or 72 hours, and then were assayed using cell counting kit-8, polymerase chain reaction (PCR) and immunobloting. The results suggested that 20 μM 5-HT treatment for 12 or 24 h promote cell viability, which was mainly induced by the activation of 5-HT receptor (5-HTR) 1B and 4, because the increase caused by 5-HT vanished when 5-HTR 1B and 4 was blocked by SB224289 and SB204070. And protein expression of mammalian target of rapamycin (mTOR), eukaryotic translation elongation factor 2 (eEF2), janus kinase 2 (JAK2) and signal transducer and activator of transcription 5 (STAT5) were decreased after blocking 5-HT 1B and 4 receptors. When MAC-T cells were treated with 5-HT and PRL simultaneously for 24 h, both the cell viability and the level of mTOR protein were significantly higher than that cultured with 5-HT or PRL alone. In conclusion, our study suggested that 5-HT promotes the viability of MAC-T cells by 5-HTR 1B and/or 4. Furthermore, there is a reciprocal relationship between PRL and 5-HT. |
format | Online Article Text |
id | pubmed-9574616 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Korean Society of Animal Sciences and Technology |
record_format | MEDLINE/PubMed |
spelling | pubmed-95746162022-10-24 Effect of serotonin on the cell viability of the bovine mammary alveolar cell-T (MAC-T) cell line Dong, Xusheng Liu, Chen Miao, Jialin Lin, Xueyan Wang, Yun Wang, Zhonghua Hou, Qiuling J Anim Sci Technol Research Article 5-Hydroxytryptamine (5-HT), a monoamine, as a local regulator in the mammary gland is a chemical signal produced by the mammary epithelium cell. In cows, studies have shown that 5-HT is associated with epithelial cell apoptosis during the degenerative phase of the mammary gland. However, studies in other tissues have shown that 5-HT can effectively promote cell viability. Whether 5-HT could have an effect on mammary cell viability in dairy cows is still unknown. The purpose of this study was to determine: (1) effect of 5-HT on the viability of bovine mammary epithelial cells and its related signaling pathways, (2) interaction between prolactin (PRL) and 5-HT on the cell viability. The bovine mammary alveolar cell-T (MAC-T) were cultured with different concentrations of 5-HT for 12, 24, 48 or 72 hours, and then were assayed using cell counting kit-8, polymerase chain reaction (PCR) and immunobloting. The results suggested that 20 μM 5-HT treatment for 12 or 24 h promote cell viability, which was mainly induced by the activation of 5-HT receptor (5-HTR) 1B and 4, because the increase caused by 5-HT vanished when 5-HTR 1B and 4 was blocked by SB224289 and SB204070. And protein expression of mammalian target of rapamycin (mTOR), eukaryotic translation elongation factor 2 (eEF2), janus kinase 2 (JAK2) and signal transducer and activator of transcription 5 (STAT5) were decreased after blocking 5-HT 1B and 4 receptors. When MAC-T cells were treated with 5-HT and PRL simultaneously for 24 h, both the cell viability and the level of mTOR protein were significantly higher than that cultured with 5-HT or PRL alone. In conclusion, our study suggested that 5-HT promotes the viability of MAC-T cells by 5-HTR 1B and/or 4. Furthermore, there is a reciprocal relationship between PRL and 5-HT. Korean Society of Animal Sciences and Technology 2022-09 2022-09-30 /pmc/articles/PMC9574616/ /pubmed/36287778 http://dx.doi.org/10.5187/jast.2022.e50 Text en © Copyright 2022 Korean Society of Animal Science and Technology https://creativecommons.org/licenses/by-nc/4.0/This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Dong, Xusheng Liu, Chen Miao, Jialin Lin, Xueyan Wang, Yun Wang, Zhonghua Hou, Qiuling Effect of serotonin on the cell viability of the bovine mammary alveolar cell-T (MAC-T) cell line |
title | Effect of serotonin on the cell viability of the bovine mammary
alveolar cell-T (MAC-T) cell line |
title_full | Effect of serotonin on the cell viability of the bovine mammary
alveolar cell-T (MAC-T) cell line |
title_fullStr | Effect of serotonin on the cell viability of the bovine mammary
alveolar cell-T (MAC-T) cell line |
title_full_unstemmed | Effect of serotonin on the cell viability of the bovine mammary
alveolar cell-T (MAC-T) cell line |
title_short | Effect of serotonin on the cell viability of the bovine mammary
alveolar cell-T (MAC-T) cell line |
title_sort | effect of serotonin on the cell viability of the bovine mammary
alveolar cell-t (mac-t) cell line |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9574616/ https://www.ncbi.nlm.nih.gov/pubmed/36287778 http://dx.doi.org/10.5187/jast.2022.e50 |
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