Quantification of Dark Protein Populations in Fluorescent Proteins by Two-Color Coincidence Detection and Nanophotonic Manipulation

[Image: see text] Genetically encoded visible fluorescent proteins (VFPs) are a key tool used to visualize cellular processes. However, compared to synthetic fluorophores, VFPs are photophysically complex. This photophysical complexity includes the presence of non-emitting, dark proteins within the...

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Detalles Bibliográficos
Autores principales: Heesink, Gobert, Caron, Cécile, van Leijenhorst-Groener, Kirsten, Molenaar, Robert, Gadella, Theodorus W. J., Claessens, Mireille M. A. E., Blum, Christian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2022
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9574928/
https://www.ncbi.nlm.nih.gov/pubmed/36190918
http://dx.doi.org/10.1021/acs.jpcb.2c04627