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Role of Delta/Notch-like epidermal growth factor-related receptor in gastric cancer patients and cells and its clinical significance

Gastric cancer (GC) is a common digestive system malignancy. The aim of this study was to explore the role of Delta/Notch-like epidermal growth factor-related receptor (DNER) in GC patients and cells. Gene expression omnibus data base public databases were used to analyze the DNER expression in GC p...

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Autores principales: Tao, Hanchuan, Wang, Cheng, Zhu, Yifan, Lu, Chongmei, Zhou, Xiaojun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Lippincott Williams & Wilkins 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9575567/
https://www.ncbi.nlm.nih.gov/pubmed/36255071
http://dx.doi.org/10.1097/CAD.0000000000001379
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author Tao, Hanchuan
Wang, Cheng
Zhu, Yifan
Lu, Chongmei
Zhou, Xiaojun
author_facet Tao, Hanchuan
Wang, Cheng
Zhu, Yifan
Lu, Chongmei
Zhou, Xiaojun
author_sort Tao, Hanchuan
collection PubMed
description Gastric cancer (GC) is a common digestive system malignancy. The aim of this study was to explore the role of Delta/Notch-like epidermal growth factor-related receptor (DNER) in GC patients and cells. Gene expression omnibus data base public databases were used to analyze the DNER expression in GC patient. A total of 30 cases of GC and adjacent tissue samples were retrospectively obtained to analyze the DNER expression. MTT assay was conducted to measure the cell viability. The apoptosis rate of GC cells was determined by flow cytometry. The migration and invasion were detected by transwell assay. Real-time polymerase chain reaction and western blot were performed to measure the DNER expression. Bioinformatics tools exhibited that DNER expression is significantly upregulated in the GC, which was also found in GC tissues and cells. The high levels of DNER were closely related the tumor size, sex and lymph node metastasis. Additionally, the survival rate of patients with high DNER expression is decreased. Furthermore, knockdown of DNER inhibits the proliferation, migration and invasion, and induces the apoptosis rate of the GC cells. DNER was upregulated in GC and knockdown of DNER inhibits the growth and metastasis of DNER. DNER may be a potential prognostic biomarker and therapeutic target of GC patients.
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spelling pubmed-95755672022-10-19 Role of Delta/Notch-like epidermal growth factor-related receptor in gastric cancer patients and cells and its clinical significance Tao, Hanchuan Wang, Cheng Zhu, Yifan Lu, Chongmei Zhou, Xiaojun Anticancer Drugs Case Reports Gastric cancer (GC) is a common digestive system malignancy. The aim of this study was to explore the role of Delta/Notch-like epidermal growth factor-related receptor (DNER) in GC patients and cells. Gene expression omnibus data base public databases were used to analyze the DNER expression in GC patient. A total of 30 cases of GC and adjacent tissue samples were retrospectively obtained to analyze the DNER expression. MTT assay was conducted to measure the cell viability. The apoptosis rate of GC cells was determined by flow cytometry. The migration and invasion were detected by transwell assay. Real-time polymerase chain reaction and western blot were performed to measure the DNER expression. Bioinformatics tools exhibited that DNER expression is significantly upregulated in the GC, which was also found in GC tissues and cells. The high levels of DNER were closely related the tumor size, sex and lymph node metastasis. Additionally, the survival rate of patients with high DNER expression is decreased. Furthermore, knockdown of DNER inhibits the proliferation, migration and invasion, and induces the apoptosis rate of the GC cells. DNER was upregulated in GC and knockdown of DNER inhibits the growth and metastasis of DNER. DNER may be a potential prognostic biomarker and therapeutic target of GC patients. Lippincott Williams & Wilkins 2022-09-29 2022-11 /pmc/articles/PMC9575567/ /pubmed/36255071 http://dx.doi.org/10.1097/CAD.0000000000001379 Text en Copyright © 2022 The Author(s). Published by Wolters Kluwer Health, Inc. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/) , where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal.
spellingShingle Case Reports
Tao, Hanchuan
Wang, Cheng
Zhu, Yifan
Lu, Chongmei
Zhou, Xiaojun
Role of Delta/Notch-like epidermal growth factor-related receptor in gastric cancer patients and cells and its clinical significance
title Role of Delta/Notch-like epidermal growth factor-related receptor in gastric cancer patients and cells and its clinical significance
title_full Role of Delta/Notch-like epidermal growth factor-related receptor in gastric cancer patients and cells and its clinical significance
title_fullStr Role of Delta/Notch-like epidermal growth factor-related receptor in gastric cancer patients and cells and its clinical significance
title_full_unstemmed Role of Delta/Notch-like epidermal growth factor-related receptor in gastric cancer patients and cells and its clinical significance
title_short Role of Delta/Notch-like epidermal growth factor-related receptor in gastric cancer patients and cells and its clinical significance
title_sort role of delta/notch-like epidermal growth factor-related receptor in gastric cancer patients and cells and its clinical significance
topic Case Reports
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9575567/
https://www.ncbi.nlm.nih.gov/pubmed/36255071
http://dx.doi.org/10.1097/CAD.0000000000001379
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