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Role of Delta/Notch-like epidermal growth factor-related receptor in gastric cancer patients and cells and its clinical significance
Gastric cancer (GC) is a common digestive system malignancy. The aim of this study was to explore the role of Delta/Notch-like epidermal growth factor-related receptor (DNER) in GC patients and cells. Gene expression omnibus data base public databases were used to analyze the DNER expression in GC p...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Lippincott Williams & Wilkins
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9575567/ https://www.ncbi.nlm.nih.gov/pubmed/36255071 http://dx.doi.org/10.1097/CAD.0000000000001379 |
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author | Tao, Hanchuan Wang, Cheng Zhu, Yifan Lu, Chongmei Zhou, Xiaojun |
author_facet | Tao, Hanchuan Wang, Cheng Zhu, Yifan Lu, Chongmei Zhou, Xiaojun |
author_sort | Tao, Hanchuan |
collection | PubMed |
description | Gastric cancer (GC) is a common digestive system malignancy. The aim of this study was to explore the role of Delta/Notch-like epidermal growth factor-related receptor (DNER) in GC patients and cells. Gene expression omnibus data base public databases were used to analyze the DNER expression in GC patient. A total of 30 cases of GC and adjacent tissue samples were retrospectively obtained to analyze the DNER expression. MTT assay was conducted to measure the cell viability. The apoptosis rate of GC cells was determined by flow cytometry. The migration and invasion were detected by transwell assay. Real-time polymerase chain reaction and western blot were performed to measure the DNER expression. Bioinformatics tools exhibited that DNER expression is significantly upregulated in the GC, which was also found in GC tissues and cells. The high levels of DNER were closely related the tumor size, sex and lymph node metastasis. Additionally, the survival rate of patients with high DNER expression is decreased. Furthermore, knockdown of DNER inhibits the proliferation, migration and invasion, and induces the apoptosis rate of the GC cells. DNER was upregulated in GC and knockdown of DNER inhibits the growth and metastasis of DNER. DNER may be a potential prognostic biomarker and therapeutic target of GC patients. |
format | Online Article Text |
id | pubmed-9575567 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Lippincott Williams & Wilkins |
record_format | MEDLINE/PubMed |
spelling | pubmed-95755672022-10-19 Role of Delta/Notch-like epidermal growth factor-related receptor in gastric cancer patients and cells and its clinical significance Tao, Hanchuan Wang, Cheng Zhu, Yifan Lu, Chongmei Zhou, Xiaojun Anticancer Drugs Case Reports Gastric cancer (GC) is a common digestive system malignancy. The aim of this study was to explore the role of Delta/Notch-like epidermal growth factor-related receptor (DNER) in GC patients and cells. Gene expression omnibus data base public databases were used to analyze the DNER expression in GC patient. A total of 30 cases of GC and adjacent tissue samples were retrospectively obtained to analyze the DNER expression. MTT assay was conducted to measure the cell viability. The apoptosis rate of GC cells was determined by flow cytometry. The migration and invasion were detected by transwell assay. Real-time polymerase chain reaction and western blot were performed to measure the DNER expression. Bioinformatics tools exhibited that DNER expression is significantly upregulated in the GC, which was also found in GC tissues and cells. The high levels of DNER were closely related the tumor size, sex and lymph node metastasis. Additionally, the survival rate of patients with high DNER expression is decreased. Furthermore, knockdown of DNER inhibits the proliferation, migration and invasion, and induces the apoptosis rate of the GC cells. DNER was upregulated in GC and knockdown of DNER inhibits the growth and metastasis of DNER. DNER may be a potential prognostic biomarker and therapeutic target of GC patients. Lippincott Williams & Wilkins 2022-09-29 2022-11 /pmc/articles/PMC9575567/ /pubmed/36255071 http://dx.doi.org/10.1097/CAD.0000000000001379 Text en Copyright © 2022 The Author(s). Published by Wolters Kluwer Health, Inc. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/) , where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal. |
spellingShingle | Case Reports Tao, Hanchuan Wang, Cheng Zhu, Yifan Lu, Chongmei Zhou, Xiaojun Role of Delta/Notch-like epidermal growth factor-related receptor in gastric cancer patients and cells and its clinical significance |
title | Role of Delta/Notch-like epidermal growth factor-related receptor in gastric cancer patients and cells and its clinical significance |
title_full | Role of Delta/Notch-like epidermal growth factor-related receptor in gastric cancer patients and cells and its clinical significance |
title_fullStr | Role of Delta/Notch-like epidermal growth factor-related receptor in gastric cancer patients and cells and its clinical significance |
title_full_unstemmed | Role of Delta/Notch-like epidermal growth factor-related receptor in gastric cancer patients and cells and its clinical significance |
title_short | Role of Delta/Notch-like epidermal growth factor-related receptor in gastric cancer patients and cells and its clinical significance |
title_sort | role of delta/notch-like epidermal growth factor-related receptor in gastric cancer patients and cells and its clinical significance |
topic | Case Reports |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9575567/ https://www.ncbi.nlm.nih.gov/pubmed/36255071 http://dx.doi.org/10.1097/CAD.0000000000001379 |
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