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Analytical sensitivity of six lateral flow antigen test kits for variant strains of SARS-CoV-2
INTRODUCTION: The lateral flow antigen test is a useful tool for rapid diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. The analytical sensitivity of six lateral flow antigen test kits was compared. METHODS: The limit of detection (LoD) and time to positive result...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Japanese Society of Chemotherapy, Japanese Association for Infectious Diseases, and Japanese Society for Infection Prevention and Control. Published by Elsevier Ltd.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9575572/ https://www.ncbi.nlm.nih.gov/pubmed/36265820 http://dx.doi.org/10.1016/j.jiac.2022.10.004 |
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author | Morinaga, Yoshitomo Yamada, Hiroshi Yoshida, Yoshihiro Kawasuji, Hitoshi Yamamoto, Yoshihiro |
author_facet | Morinaga, Yoshitomo Yamada, Hiroshi Yoshida, Yoshihiro Kawasuji, Hitoshi Yamamoto, Yoshihiro |
author_sort | Morinaga, Yoshitomo |
collection | PubMed |
description | INTRODUCTION: The lateral flow antigen test is a useful tool for rapid diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. The analytical sensitivity of six lateral flow antigen test kits was compared. METHODS: The limit of detection (LoD) and time to positive results were evaluated for six lateral flow tests including ImmunoArrow®, ESPLINE® SARS-CoV-2, QuickNavi™ COVID19 Ag, ImmunoAce® SARS-CoV-2, Panbio™ COVID-19 Ag Rapid Test Device, and SARS-CoV-2 Rapid Antigen Test using the heat-inactivated virus. The LoD of ImmunoArrow® against the Omicron variants was compared with that against the wild-type using recombinant proteins. RESULTS: ImmunoArrow® and ESPLINE® showed the lowest LoD. The time to positive results of all tests except for ESPLINE® was within 200 s in the evaluation at high dose of antigens (2.5 × 10(5) TCID(50)/mL) and 500 s in the evaluation at low dose of antigens (2.5 × 10(4) TCID(50)/mL). The LoD of ImmunoArrow® against the Omicron variants was the same concentration against the wild-type antigen. CONCLUSIONS: ImmunoArrow® detected SARS-CoV-2 antigens including the Omicron variants with good sensitivity among the six lateral flow antigen tests. These finding support that it can support the rapid diagnosis of COVID-19 with the good sensitivity. |
format | Online Article Text |
id | pubmed-9575572 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Japanese Society of Chemotherapy, Japanese Association for Infectious Diseases, and Japanese Society for Infection Prevention and Control. Published by Elsevier Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-95755722022-10-17 Analytical sensitivity of six lateral flow antigen test kits for variant strains of SARS-CoV-2 Morinaga, Yoshitomo Yamada, Hiroshi Yoshida, Yoshihiro Kawasuji, Hitoshi Yamamoto, Yoshihiro J Infect Chemother Original Article INTRODUCTION: The lateral flow antigen test is a useful tool for rapid diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. The analytical sensitivity of six lateral flow antigen test kits was compared. METHODS: The limit of detection (LoD) and time to positive results were evaluated for six lateral flow tests including ImmunoArrow®, ESPLINE® SARS-CoV-2, QuickNavi™ COVID19 Ag, ImmunoAce® SARS-CoV-2, Panbio™ COVID-19 Ag Rapid Test Device, and SARS-CoV-2 Rapid Antigen Test using the heat-inactivated virus. The LoD of ImmunoArrow® against the Omicron variants was compared with that against the wild-type using recombinant proteins. RESULTS: ImmunoArrow® and ESPLINE® showed the lowest LoD. The time to positive results of all tests except for ESPLINE® was within 200 s in the evaluation at high dose of antigens (2.5 × 10(5) TCID(50)/mL) and 500 s in the evaluation at low dose of antigens (2.5 × 10(4) TCID(50)/mL). The LoD of ImmunoArrow® against the Omicron variants was the same concentration against the wild-type antigen. CONCLUSIONS: ImmunoArrow® detected SARS-CoV-2 antigens including the Omicron variants with good sensitivity among the six lateral flow antigen tests. These finding support that it can support the rapid diagnosis of COVID-19 with the good sensitivity. Japanese Society of Chemotherapy, Japanese Association for Infectious Diseases, and Japanese Society for Infection Prevention and Control. Published by Elsevier Ltd. 2023-02 2022-10-17 /pmc/articles/PMC9575572/ /pubmed/36265820 http://dx.doi.org/10.1016/j.jiac.2022.10.004 Text en © 2023 Japanese Society of Chemotherapy, Japanese Association for Infectious Diseases, and Japanese Society for Infection Prevention and Control. Published by Elsevier Ltd. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Original Article Morinaga, Yoshitomo Yamada, Hiroshi Yoshida, Yoshihiro Kawasuji, Hitoshi Yamamoto, Yoshihiro Analytical sensitivity of six lateral flow antigen test kits for variant strains of SARS-CoV-2 |
title | Analytical sensitivity of six lateral flow antigen test kits for variant strains of SARS-CoV-2 |
title_full | Analytical sensitivity of six lateral flow antigen test kits for variant strains of SARS-CoV-2 |
title_fullStr | Analytical sensitivity of six lateral flow antigen test kits for variant strains of SARS-CoV-2 |
title_full_unstemmed | Analytical sensitivity of six lateral flow antigen test kits for variant strains of SARS-CoV-2 |
title_short | Analytical sensitivity of six lateral flow antigen test kits for variant strains of SARS-CoV-2 |
title_sort | analytical sensitivity of six lateral flow antigen test kits for variant strains of sars-cov-2 |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9575572/ https://www.ncbi.nlm.nih.gov/pubmed/36265820 http://dx.doi.org/10.1016/j.jiac.2022.10.004 |
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