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Virulence characteristics of Blumeria graminis f. sp. tritici and its genetic diversity by EST-SSR analyses

Wheat powdery mildew, caused by Blumeria graminis f. sp. tritici (an obligate biotrophic pathogen) is a worldwide threat to wheat production that occurs over a wide geographic area in China. For monitoring genetic variation and virulence structure of Blumeria graminis f. sp. tritici in Liaoning, Hei...

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Autores principales: Zhang, Yazhao, Wu, Xianxin, Wang, Wanlin, Xu, Yiwei, Sun, Huiyan, Cao, Yuanyin, Li, Tianya, Karimi-Jashni, Mansoor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9575677/
https://www.ncbi.nlm.nih.gov/pubmed/36262408
http://dx.doi.org/10.7717/peerj.14118
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author Zhang, Yazhao
Wu, Xianxin
Wang, Wanlin
Xu, Yiwei
Sun, Huiyan
Cao, Yuanyin
Li, Tianya
Karimi-Jashni, Mansoor
author_facet Zhang, Yazhao
Wu, Xianxin
Wang, Wanlin
Xu, Yiwei
Sun, Huiyan
Cao, Yuanyin
Li, Tianya
Karimi-Jashni, Mansoor
author_sort Zhang, Yazhao
collection PubMed
description Wheat powdery mildew, caused by Blumeria graminis f. sp. tritici (an obligate biotrophic pathogen) is a worldwide threat to wheat production that occurs over a wide geographic area in China. For monitoring genetic variation and virulence structure of Blumeria graminis f. sp. tritici in Liaoning, Heilongjiang, and Sichuan in 2015, 31 wheat lines with known Powdery mildew resistance genes and 2 EST-SSR markers were used to characterize the virulence and genetic diversity. Results indicated that 90% of all isolates were virulent on Pm3c, Pm3e, Pm3f, Pm4a, Pm5, Pm6 (Timgalen), Pm7, Pm16, Pm19, and Pm1 + 2 + 9 and 62.6% to 89.9% of isolates were virulent on Pm3a, Pm3b, Pm3d, Pm4b, Pm6 (Coker747), Pm8, Pm17, Pm20, Pm23, Pm30, Pm4 + 8, Pm5 + 6, Pm4b + mli, Pm2 + mld, Pm4 + 2X, Pm2 + 6. The Pm13 and PmXBD genes were effective against most collected isolates from Liaoning and Heilongjiang Provinces. Only Pm21 exhibited an immune infection response to all isolates. Furthermore, closely related isolates within each region were distinguished by cluster analyses using EST-SSR representing some gene exchanges and genetic relationships between the flora in Northeast China (Liaoning, Heilongjiang) and Sichuan. Only 45% of the isolates tested show a clear correlation between EST-SSR genetic polymorphisms and the frequency of virulence gene data. However, the EST-SSR polymorphism of isolated genes did not correspond to the virulence diversity of isolates in the single-gene lineage identification of hosts.
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spelling pubmed-95756772022-10-18 Virulence characteristics of Blumeria graminis f. sp. tritici and its genetic diversity by EST-SSR analyses Zhang, Yazhao Wu, Xianxin Wang, Wanlin Xu, Yiwei Sun, Huiyan Cao, Yuanyin Li, Tianya Karimi-Jashni, Mansoor PeerJ Agricultural Science Wheat powdery mildew, caused by Blumeria graminis f. sp. tritici (an obligate biotrophic pathogen) is a worldwide threat to wheat production that occurs over a wide geographic area in China. For monitoring genetic variation and virulence structure of Blumeria graminis f. sp. tritici in Liaoning, Heilongjiang, and Sichuan in 2015, 31 wheat lines with known Powdery mildew resistance genes and 2 EST-SSR markers were used to characterize the virulence and genetic diversity. Results indicated that 90% of all isolates were virulent on Pm3c, Pm3e, Pm3f, Pm4a, Pm5, Pm6 (Timgalen), Pm7, Pm16, Pm19, and Pm1 + 2 + 9 and 62.6% to 89.9% of isolates were virulent on Pm3a, Pm3b, Pm3d, Pm4b, Pm6 (Coker747), Pm8, Pm17, Pm20, Pm23, Pm30, Pm4 + 8, Pm5 + 6, Pm4b + mli, Pm2 + mld, Pm4 + 2X, Pm2 + 6. The Pm13 and PmXBD genes were effective against most collected isolates from Liaoning and Heilongjiang Provinces. Only Pm21 exhibited an immune infection response to all isolates. Furthermore, closely related isolates within each region were distinguished by cluster analyses using EST-SSR representing some gene exchanges and genetic relationships between the flora in Northeast China (Liaoning, Heilongjiang) and Sichuan. Only 45% of the isolates tested show a clear correlation between EST-SSR genetic polymorphisms and the frequency of virulence gene data. However, the EST-SSR polymorphism of isolated genes did not correspond to the virulence diversity of isolates in the single-gene lineage identification of hosts. PeerJ Inc. 2022-10-14 /pmc/articles/PMC9575677/ /pubmed/36262408 http://dx.doi.org/10.7717/peerj.14118 Text en © 2022 Zhang et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Agricultural Science
Zhang, Yazhao
Wu, Xianxin
Wang, Wanlin
Xu, Yiwei
Sun, Huiyan
Cao, Yuanyin
Li, Tianya
Karimi-Jashni, Mansoor
Virulence characteristics of Blumeria graminis f. sp. tritici and its genetic diversity by EST-SSR analyses
title Virulence characteristics of Blumeria graminis f. sp. tritici and its genetic diversity by EST-SSR analyses
title_full Virulence characteristics of Blumeria graminis f. sp. tritici and its genetic diversity by EST-SSR analyses
title_fullStr Virulence characteristics of Blumeria graminis f. sp. tritici and its genetic diversity by EST-SSR analyses
title_full_unstemmed Virulence characteristics of Blumeria graminis f. sp. tritici and its genetic diversity by EST-SSR analyses
title_short Virulence characteristics of Blumeria graminis f. sp. tritici and its genetic diversity by EST-SSR analyses
title_sort virulence characteristics of blumeria graminis f. sp. tritici and its genetic diversity by est-ssr analyses
topic Agricultural Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9575677/
https://www.ncbi.nlm.nih.gov/pubmed/36262408
http://dx.doi.org/10.7717/peerj.14118
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