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Small Intestine-specific Knockout of CIDEC Improves Obesity and Hepatic Steatosis by Inhibiting Synthesis of Phosphatidic Acid
The small intestine is main site of exogenous lipid digestion and absorption, and it is important for lipid metabolic homeostasis. Cell death-inducing DNA fragmentation-factor like effector C (CIDEC) is active in lipid metabolism in tissues other than those in the intestine. We developed small intes...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Ivyspring International Publisher
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9576510/ https://www.ncbi.nlm.nih.gov/pubmed/36263170 http://dx.doi.org/10.7150/ijbs.74348 |
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author | Huang, Liang Liao, Qichao Pan, Tingli Sun, Yu Aluo, Zhier Xiao, Lianggui Yu, Jingsu Liu, Siqi Xiao, Yang Yang, Yufeng Li, Yixing Zhou, Lei |
author_facet | Huang, Liang Liao, Qichao Pan, Tingli Sun, Yu Aluo, Zhier Xiao, Lianggui Yu, Jingsu Liu, Siqi Xiao, Yang Yang, Yufeng Li, Yixing Zhou, Lei |
author_sort | Huang, Liang |
collection | PubMed |
description | The small intestine is main site of exogenous lipid digestion and absorption, and it is important for lipid metabolic homeostasis. Cell death-inducing DNA fragmentation-factor like effector C (CIDEC) is active in lipid metabolism in tissues other than those in the intestine. We developed small intestine-specific CIDEC (SI-CIDEC(-/-)) knockout C57BL/6J mice by Cre/LoxP recombination to investigate the in vivo effects of intestinal CIDEC on lipid metabolism. Eight-week-old SI-CIDEC(-/-) mice fed a high-fat diet for 14 weeks had 15% lower body weight, 30% less body fat mass, and 79% lower liver triglycerides (TG) than wild-type (WT) mice. In addition, hepatic steatosis and fatty liver inflammation were less severe in knockout mice fed a high-fat diet (HFD) compared with wild-type mice fed an HFD. SI-CIDEC(-/-) mice fed an HFD diet had lower serum TG and higher fecal TG and intestinal lipase activity than wild-type mice. Mechanistic studies showed that CIDEC accelerated phosphatidic acid synthesis by interacting with 1-acylglycerol-3-phosphate-O-acyltransferase to promote TG accumulation. This study identified a new interacting protein and previously unreported CIDEC mechanisms that revealed its activity in lipid metabolism of the small intestine. |
format | Online Article Text |
id | pubmed-9576510 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Ivyspring International Publisher |
record_format | MEDLINE/PubMed |
spelling | pubmed-95765102022-10-18 Small Intestine-specific Knockout of CIDEC Improves Obesity and Hepatic Steatosis by Inhibiting Synthesis of Phosphatidic Acid Huang, Liang Liao, Qichao Pan, Tingli Sun, Yu Aluo, Zhier Xiao, Lianggui Yu, Jingsu Liu, Siqi Xiao, Yang Yang, Yufeng Li, Yixing Zhou, Lei Int J Biol Sci Research Paper The small intestine is main site of exogenous lipid digestion and absorption, and it is important for lipid metabolic homeostasis. Cell death-inducing DNA fragmentation-factor like effector C (CIDEC) is active in lipid metabolism in tissues other than those in the intestine. We developed small intestine-specific CIDEC (SI-CIDEC(-/-)) knockout C57BL/6J mice by Cre/LoxP recombination to investigate the in vivo effects of intestinal CIDEC on lipid metabolism. Eight-week-old SI-CIDEC(-/-) mice fed a high-fat diet for 14 weeks had 15% lower body weight, 30% less body fat mass, and 79% lower liver triglycerides (TG) than wild-type (WT) mice. In addition, hepatic steatosis and fatty liver inflammation were less severe in knockout mice fed a high-fat diet (HFD) compared with wild-type mice fed an HFD. SI-CIDEC(-/-) mice fed an HFD diet had lower serum TG and higher fecal TG and intestinal lipase activity than wild-type mice. Mechanistic studies showed that CIDEC accelerated phosphatidic acid synthesis by interacting with 1-acylglycerol-3-phosphate-O-acyltransferase to promote TG accumulation. This study identified a new interacting protein and previously unreported CIDEC mechanisms that revealed its activity in lipid metabolism of the small intestine. Ivyspring International Publisher 2022-09-11 /pmc/articles/PMC9576510/ /pubmed/36263170 http://dx.doi.org/10.7150/ijbs.74348 Text en © The author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions. |
spellingShingle | Research Paper Huang, Liang Liao, Qichao Pan, Tingli Sun, Yu Aluo, Zhier Xiao, Lianggui Yu, Jingsu Liu, Siqi Xiao, Yang Yang, Yufeng Li, Yixing Zhou, Lei Small Intestine-specific Knockout of CIDEC Improves Obesity and Hepatic Steatosis by Inhibiting Synthesis of Phosphatidic Acid |
title | Small Intestine-specific Knockout of CIDEC Improves Obesity and Hepatic Steatosis by Inhibiting Synthesis of Phosphatidic Acid |
title_full | Small Intestine-specific Knockout of CIDEC Improves Obesity and Hepatic Steatosis by Inhibiting Synthesis of Phosphatidic Acid |
title_fullStr | Small Intestine-specific Knockout of CIDEC Improves Obesity and Hepatic Steatosis by Inhibiting Synthesis of Phosphatidic Acid |
title_full_unstemmed | Small Intestine-specific Knockout of CIDEC Improves Obesity and Hepatic Steatosis by Inhibiting Synthesis of Phosphatidic Acid |
title_short | Small Intestine-specific Knockout of CIDEC Improves Obesity and Hepatic Steatosis by Inhibiting Synthesis of Phosphatidic Acid |
title_sort | small intestine-specific knockout of cidec improves obesity and hepatic steatosis by inhibiting synthesis of phosphatidic acid |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9576510/ https://www.ncbi.nlm.nih.gov/pubmed/36263170 http://dx.doi.org/10.7150/ijbs.74348 |
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