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Quantitative proteomic analysis provides insight into the survival mechanism of Salmonella typhimurium under high-intensity ultrasound treatment

The survival mechanism of Salmonella treated with high-intensity ultrasound (HIU) should be explored to further enhance the bactericidal efficacy of HIU. In this study, culturable Salmonella was reduced by applying HIU. Electron microscope imaging revealed that HIU caused the disintegration of cell...

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Autores principales: Luo, Wei, Wang, Jinqiu, Chen, Yan, Wang, Yixu, Li, Rui, Tang, Jie, Geng, Fang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9576580/
https://www.ncbi.nlm.nih.gov/pubmed/36268134
http://dx.doi.org/10.1016/j.crfs.2022.09.029
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author Luo, Wei
Wang, Jinqiu
Chen, Yan
Wang, Yixu
Li, Rui
Tang, Jie
Geng, Fang
author_facet Luo, Wei
Wang, Jinqiu
Chen, Yan
Wang, Yixu
Li, Rui
Tang, Jie
Geng, Fang
author_sort Luo, Wei
collection PubMed
description The survival mechanism of Salmonella treated with high-intensity ultrasound (HIU) should be explored to further enhance the bactericidal efficacy of HIU. In this study, culturable Salmonella was reduced by applying HIU. Electron microscope imaging revealed that HIU caused the disintegration of cell structure and leakage of intracellular substances. For the Salmonella after the HIU treatment, key enzymes of the tricarboxylic acid [TCA] cycle were significantly downregulated, which led to a reduced ATP content (45.25%–75.00%), although ATPase activity was augmented by 33.82%–60.64% in the Salmonella. Accordingly, surviving Salmonella could have tolerated the stress of HIU by upregulating their environmental sensing (two-component system), chemotaxis (bacterial chemotaxis), substance uptake (ABC transporter), and ATP production (oxidative phosphorylation). Therefore, synergistically blocking the ATP production, signal transduction, or substance intake of Salmonella offer promising potential strategies to improve the bactericidal effect of HIU in industrial food processing.
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spelling pubmed-95765802022-10-19 Quantitative proteomic analysis provides insight into the survival mechanism of Salmonella typhimurium under high-intensity ultrasound treatment Luo, Wei Wang, Jinqiu Chen, Yan Wang, Yixu Li, Rui Tang, Jie Geng, Fang Curr Res Food Sci Research Article The survival mechanism of Salmonella treated with high-intensity ultrasound (HIU) should be explored to further enhance the bactericidal efficacy of HIU. In this study, culturable Salmonella was reduced by applying HIU. Electron microscope imaging revealed that HIU caused the disintegration of cell structure and leakage of intracellular substances. For the Salmonella after the HIU treatment, key enzymes of the tricarboxylic acid [TCA] cycle were significantly downregulated, which led to a reduced ATP content (45.25%–75.00%), although ATPase activity was augmented by 33.82%–60.64% in the Salmonella. Accordingly, surviving Salmonella could have tolerated the stress of HIU by upregulating their environmental sensing (two-component system), chemotaxis (bacterial chemotaxis), substance uptake (ABC transporter), and ATP production (oxidative phosphorylation). Therefore, synergistically blocking the ATP production, signal transduction, or substance intake of Salmonella offer promising potential strategies to improve the bactericidal effect of HIU in industrial food processing. Elsevier 2022-10-09 /pmc/articles/PMC9576580/ /pubmed/36268134 http://dx.doi.org/10.1016/j.crfs.2022.09.029 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Luo, Wei
Wang, Jinqiu
Chen, Yan
Wang, Yixu
Li, Rui
Tang, Jie
Geng, Fang
Quantitative proteomic analysis provides insight into the survival mechanism of Salmonella typhimurium under high-intensity ultrasound treatment
title Quantitative proteomic analysis provides insight into the survival mechanism of Salmonella typhimurium under high-intensity ultrasound treatment
title_full Quantitative proteomic analysis provides insight into the survival mechanism of Salmonella typhimurium under high-intensity ultrasound treatment
title_fullStr Quantitative proteomic analysis provides insight into the survival mechanism of Salmonella typhimurium under high-intensity ultrasound treatment
title_full_unstemmed Quantitative proteomic analysis provides insight into the survival mechanism of Salmonella typhimurium under high-intensity ultrasound treatment
title_short Quantitative proteomic analysis provides insight into the survival mechanism of Salmonella typhimurium under high-intensity ultrasound treatment
title_sort quantitative proteomic analysis provides insight into the survival mechanism of salmonella typhimurium under high-intensity ultrasound treatment
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9576580/
https://www.ncbi.nlm.nih.gov/pubmed/36268134
http://dx.doi.org/10.1016/j.crfs.2022.09.029
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