Protocols for the social transfer of pain and analgesia in mice

We provide protocols for the social transfer of pain and analgesia in mice. We describe the steps to induce pain or analgesia (pain relief) in bystander mice with a 1-h social interaction with a partner injected with CFA (complete Freund’s adjuvant) or CFA and morphine, respectively. We detail behav...

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Autores principales: Rein, Benjamin, Jones, Erin, Tuy, Sabrena, Boustani, Cali, Johnson, Julia A., Malenka, Robert C., Smith, Monique L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9576629/
https://www.ncbi.nlm.nih.gov/pubmed/36227742
http://dx.doi.org/10.1016/j.xpro.2022.101756
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author Rein, Benjamin
Jones, Erin
Tuy, Sabrena
Boustani, Cali
Johnson, Julia A.
Malenka, Robert C.
Smith, Monique L.
author_facet Rein, Benjamin
Jones, Erin
Tuy, Sabrena
Boustani, Cali
Johnson, Julia A.
Malenka, Robert C.
Smith, Monique L.
author_sort Rein, Benjamin
collection PubMed
description We provide protocols for the social transfer of pain and analgesia in mice. We describe the steps to induce pain or analgesia (pain relief) in bystander mice with a 1-h social interaction with a partner injected with CFA (complete Freund’s adjuvant) or CFA and morphine, respectively. We detail behavioral tests to assess pain or analgesia in the untreated bystander mice. This protocol has been validated in mice and rats and can be used for investigating mechanisms of empathy. For complete details on the use and execution of this protocol, please refer to Smith et al. (2021).
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spelling pubmed-95766292022-10-19 Protocols for the social transfer of pain and analgesia in mice Rein, Benjamin Jones, Erin Tuy, Sabrena Boustani, Cali Johnson, Julia A. Malenka, Robert C. Smith, Monique L. STAR Protoc Protocol We provide protocols for the social transfer of pain and analgesia in mice. We describe the steps to induce pain or analgesia (pain relief) in bystander mice with a 1-h social interaction with a partner injected with CFA (complete Freund’s adjuvant) or CFA and morphine, respectively. We detail behavioral tests to assess pain or analgesia in the untreated bystander mice. This protocol has been validated in mice and rats and can be used for investigating mechanisms of empathy. For complete details on the use and execution of this protocol, please refer to Smith et al. (2021). Elsevier 2022-10-12 /pmc/articles/PMC9576629/ /pubmed/36227742 http://dx.doi.org/10.1016/j.xpro.2022.101756 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Rein, Benjamin
Jones, Erin
Tuy, Sabrena
Boustani, Cali
Johnson, Julia A.
Malenka, Robert C.
Smith, Monique L.
Protocols for the social transfer of pain and analgesia in mice
title Protocols for the social transfer of pain and analgesia in mice
title_full Protocols for the social transfer of pain and analgesia in mice
title_fullStr Protocols for the social transfer of pain and analgesia in mice
title_full_unstemmed Protocols for the social transfer of pain and analgesia in mice
title_short Protocols for the social transfer of pain and analgesia in mice
title_sort protocols for the social transfer of pain and analgesia in mice
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9576629/
https://www.ncbi.nlm.nih.gov/pubmed/36227742
http://dx.doi.org/10.1016/j.xpro.2022.101756
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