Isolation, culture, and cryosectioning of primary porcine retinal pigment epithelium on transwell cell culture inserts

Primary culture and long-term maintenance of primary retinal pigment epithelium (RPE) is a useful model system for the study of ocular pathologies such as age-related macular degeneration. Here, we detail the steps for the isolation and long-term culture of primary porcine RPE. We also describe step...

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Detalles Bibliográficos
Autores principales: Hood, Erika M.S., Curcio, Christine A., Lipinski, Daniel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9576630/
https://www.ncbi.nlm.nih.gov/pubmed/36227744
http://dx.doi.org/10.1016/j.xpro.2022.101758

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9576630/
https://www.ncbi.nlm.nih.gov/pubmed/36227744
http://dx.doi.org/10.1016/j.xpro.2022.101758

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