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Isolation, culture, and cryosectioning of primary porcine retinal pigment epithelium on transwell cell culture inserts
Primary culture and long-term maintenance of primary retinal pigment epithelium (RPE) is a useful model system for the study of ocular pathologies such as age-related macular degeneration. Here, we detail the steps for the isolation and long-term culture of primary porcine RPE. We also describe step...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9576630/ https://www.ncbi.nlm.nih.gov/pubmed/36227744 http://dx.doi.org/10.1016/j.xpro.2022.101758 |