Characterizing diversity among human oral stem-like cancer cells using flow cytometry

We have developed the protocol of flow cytometry for characterizing diversity among oral stem-like cancer cells (SLCCs) using CD44, CD24, and aldehyde dehydrogenase (ALDH) in oral tumors. We are also reporting the protocol for tumor-derived explant cultures to develop oral cancer cell lines and enri...

Descripción completa

Detalles Bibliográficos
Autores principales: Ghosh, Subhashis, Mitra, Paromita, Saha, Uday, Vipparthi, Kavya, Singh, Sandeep
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9576741/
https://www.ncbi.nlm.nih.gov/pubmed/36223267
http://dx.doi.org/10.1016/j.xpro.2022.101763
Descripción
Sumario:We have developed the protocol of flow cytometry for characterizing diversity among oral stem-like cancer cells (SLCCs) using CD44, CD24, and aldehyde dehydrogenase (ALDH) in oral tumors. We are also reporting the protocol for tumor-derived explant cultures to develop oral cancer cell lines and enriching these diverse hybrid states of cancer cells in 3D spheroids from established cell lines. For complete details on the use and execution of this protocol, please refer to Vipparthi et al. (2022) and Vipparthi et al. (2021).

Ejemplares similares