Characterizing diversity among human oral stem-like cancer cells using flow cytometry

We have developed the protocol of flow cytometry for characterizing diversity among oral stem-like cancer cells (SLCCs) using CD44, CD24, and aldehyde dehydrogenase (ALDH) in oral tumors. We are also reporting the protocol for tumor-derived explant cultures to develop oral cancer cell lines and enri...

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Autores principales: Ghosh, Subhashis, Mitra, Paromita, Saha, Uday, Vipparthi, Kavya, Singh, Sandeep
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9576741/
https://www.ncbi.nlm.nih.gov/pubmed/36223267
http://dx.doi.org/10.1016/j.xpro.2022.101763
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author Ghosh, Subhashis
Mitra, Paromita
Saha, Uday
Vipparthi, Kavya
Singh, Sandeep
author_facet Ghosh, Subhashis
Mitra, Paromita
Saha, Uday
Vipparthi, Kavya
Singh, Sandeep
author_sort Ghosh, Subhashis
collection PubMed
description We have developed the protocol of flow cytometry for characterizing diversity among oral stem-like cancer cells (SLCCs) using CD44, CD24, and aldehyde dehydrogenase (ALDH) in oral tumors. We are also reporting the protocol for tumor-derived explant cultures to develop oral cancer cell lines and enriching these diverse hybrid states of cancer cells in 3D spheroids from established cell lines. For complete details on the use and execution of this protocol, please refer to Vipparthi et al. (2022) and Vipparthi et al. (2021).
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spelling pubmed-95767412022-10-19 Characterizing diversity among human oral stem-like cancer cells using flow cytometry Ghosh, Subhashis Mitra, Paromita Saha, Uday Vipparthi, Kavya Singh, Sandeep STAR Protoc Protocol We have developed the protocol of flow cytometry for characterizing diversity among oral stem-like cancer cells (SLCCs) using CD44, CD24, and aldehyde dehydrogenase (ALDH) in oral tumors. We are also reporting the protocol for tumor-derived explant cultures to develop oral cancer cell lines and enriching these diverse hybrid states of cancer cells in 3D spheroids from established cell lines. For complete details on the use and execution of this protocol, please refer to Vipparthi et al. (2022) and Vipparthi et al. (2021). Elsevier 2022-10-12 /pmc/articles/PMC9576741/ /pubmed/36223267 http://dx.doi.org/10.1016/j.xpro.2022.101763 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Ghosh, Subhashis
Mitra, Paromita
Saha, Uday
Vipparthi, Kavya
Singh, Sandeep
Characterizing diversity among human oral stem-like cancer cells using flow cytometry
title Characterizing diversity among human oral stem-like cancer cells using flow cytometry
title_full Characterizing diversity among human oral stem-like cancer cells using flow cytometry
title_fullStr Characterizing diversity among human oral stem-like cancer cells using flow cytometry
title_full_unstemmed Characterizing diversity among human oral stem-like cancer cells using flow cytometry
title_short Characterizing diversity among human oral stem-like cancer cells using flow cytometry
title_sort characterizing diversity among human oral stem-like cancer cells using flow cytometry
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9576741/
https://www.ncbi.nlm.nih.gov/pubmed/36223267
http://dx.doi.org/10.1016/j.xpro.2022.101763
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