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Dephosphorylation in nuclear reassembly after mitosis

In most animal cell types, the interphase nucleus is largely disassembled during mitotic entry. The nuclear envelope breaks down and chromosomes are compacted into separated masses. Chromatin organization is also mostly lost and kinetochores assemble on centromeres. Mitotic protein kinases play seve...

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Autores principales: Archambault, Vincent, Li, Jingjing, Emond-Fraser, Virginie, Larouche, Myreille
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9576876/
https://www.ncbi.nlm.nih.gov/pubmed/36268509
http://dx.doi.org/10.3389/fcell.2022.1012768
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author Archambault, Vincent
Li, Jingjing
Emond-Fraser, Virginie
Larouche, Myreille
author_facet Archambault, Vincent
Li, Jingjing
Emond-Fraser, Virginie
Larouche, Myreille
author_sort Archambault, Vincent
collection PubMed
description In most animal cell types, the interphase nucleus is largely disassembled during mitotic entry. The nuclear envelope breaks down and chromosomes are compacted into separated masses. Chromatin organization is also mostly lost and kinetochores assemble on centromeres. Mitotic protein kinases play several roles in inducing these transformations by phosphorylating multiple effector proteins. In many of these events, the mechanistic consequences of phosphorylation have been characterized. In comparison, how the nucleus reassembles at the end of mitosis is less well understood in mechanistic terms. In recent years, much progress has been made in deciphering how dephosphorylation of several effector proteins promotes nuclear envelope reassembly, chromosome decondensation, kinetochore disassembly and interphase chromatin organization. The precise roles of protein phosphatases in this process, in particular of the PP1 and PP2A groups, are emerging. Moreover, how these enzymes are temporally and spatially regulated to ensure that nuclear reassembly progresses in a coordinated manner has been partly uncovered. This review provides a global view of nuclear reassembly with a focus on the roles of dephosphorylation events. It also identifies important open questions and proposes hypotheses.
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spelling pubmed-95768762022-10-19 Dephosphorylation in nuclear reassembly after mitosis Archambault, Vincent Li, Jingjing Emond-Fraser, Virginie Larouche, Myreille Front Cell Dev Biol Cell and Developmental Biology In most animal cell types, the interphase nucleus is largely disassembled during mitotic entry. The nuclear envelope breaks down and chromosomes are compacted into separated masses. Chromatin organization is also mostly lost and kinetochores assemble on centromeres. Mitotic protein kinases play several roles in inducing these transformations by phosphorylating multiple effector proteins. In many of these events, the mechanistic consequences of phosphorylation have been characterized. In comparison, how the nucleus reassembles at the end of mitosis is less well understood in mechanistic terms. In recent years, much progress has been made in deciphering how dephosphorylation of several effector proteins promotes nuclear envelope reassembly, chromosome decondensation, kinetochore disassembly and interphase chromatin organization. The precise roles of protein phosphatases in this process, in particular of the PP1 and PP2A groups, are emerging. Moreover, how these enzymes are temporally and spatially regulated to ensure that nuclear reassembly progresses in a coordinated manner has been partly uncovered. This review provides a global view of nuclear reassembly with a focus on the roles of dephosphorylation events. It also identifies important open questions and proposes hypotheses. Frontiers Media S.A. 2022-10-04 /pmc/articles/PMC9576876/ /pubmed/36268509 http://dx.doi.org/10.3389/fcell.2022.1012768 Text en Copyright © 2022 Archambault, Li, Emond-Fraser and Larouche. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Archambault, Vincent
Li, Jingjing
Emond-Fraser, Virginie
Larouche, Myreille
Dephosphorylation in nuclear reassembly after mitosis
title Dephosphorylation in nuclear reassembly after mitosis
title_full Dephosphorylation in nuclear reassembly after mitosis
title_fullStr Dephosphorylation in nuclear reassembly after mitosis
title_full_unstemmed Dephosphorylation in nuclear reassembly after mitosis
title_short Dephosphorylation in nuclear reassembly after mitosis
title_sort dephosphorylation in nuclear reassembly after mitosis
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9576876/
https://www.ncbi.nlm.nih.gov/pubmed/36268509
http://dx.doi.org/10.3389/fcell.2022.1012768
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