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Dephosphorylation in nuclear reassembly after mitosis
In most animal cell types, the interphase nucleus is largely disassembled during mitotic entry. The nuclear envelope breaks down and chromosomes are compacted into separated masses. Chromatin organization is also mostly lost and kinetochores assemble on centromeres. Mitotic protein kinases play seve...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9576876/ https://www.ncbi.nlm.nih.gov/pubmed/36268509 http://dx.doi.org/10.3389/fcell.2022.1012768 |
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author | Archambault, Vincent Li, Jingjing Emond-Fraser, Virginie Larouche, Myreille |
author_facet | Archambault, Vincent Li, Jingjing Emond-Fraser, Virginie Larouche, Myreille |
author_sort | Archambault, Vincent |
collection | PubMed |
description | In most animal cell types, the interphase nucleus is largely disassembled during mitotic entry. The nuclear envelope breaks down and chromosomes are compacted into separated masses. Chromatin organization is also mostly lost and kinetochores assemble on centromeres. Mitotic protein kinases play several roles in inducing these transformations by phosphorylating multiple effector proteins. In many of these events, the mechanistic consequences of phosphorylation have been characterized. In comparison, how the nucleus reassembles at the end of mitosis is less well understood in mechanistic terms. In recent years, much progress has been made in deciphering how dephosphorylation of several effector proteins promotes nuclear envelope reassembly, chromosome decondensation, kinetochore disassembly and interphase chromatin organization. The precise roles of protein phosphatases in this process, in particular of the PP1 and PP2A groups, are emerging. Moreover, how these enzymes are temporally and spatially regulated to ensure that nuclear reassembly progresses in a coordinated manner has been partly uncovered. This review provides a global view of nuclear reassembly with a focus on the roles of dephosphorylation events. It also identifies important open questions and proposes hypotheses. |
format | Online Article Text |
id | pubmed-9576876 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-95768762022-10-19 Dephosphorylation in nuclear reassembly after mitosis Archambault, Vincent Li, Jingjing Emond-Fraser, Virginie Larouche, Myreille Front Cell Dev Biol Cell and Developmental Biology In most animal cell types, the interphase nucleus is largely disassembled during mitotic entry. The nuclear envelope breaks down and chromosomes are compacted into separated masses. Chromatin organization is also mostly lost and kinetochores assemble on centromeres. Mitotic protein kinases play several roles in inducing these transformations by phosphorylating multiple effector proteins. In many of these events, the mechanistic consequences of phosphorylation have been characterized. In comparison, how the nucleus reassembles at the end of mitosis is less well understood in mechanistic terms. In recent years, much progress has been made in deciphering how dephosphorylation of several effector proteins promotes nuclear envelope reassembly, chromosome decondensation, kinetochore disassembly and interphase chromatin organization. The precise roles of protein phosphatases in this process, in particular of the PP1 and PP2A groups, are emerging. Moreover, how these enzymes are temporally and spatially regulated to ensure that nuclear reassembly progresses in a coordinated manner has been partly uncovered. This review provides a global view of nuclear reassembly with a focus on the roles of dephosphorylation events. It also identifies important open questions and proposes hypotheses. Frontiers Media S.A. 2022-10-04 /pmc/articles/PMC9576876/ /pubmed/36268509 http://dx.doi.org/10.3389/fcell.2022.1012768 Text en Copyright © 2022 Archambault, Li, Emond-Fraser and Larouche. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cell and Developmental Biology Archambault, Vincent Li, Jingjing Emond-Fraser, Virginie Larouche, Myreille Dephosphorylation in nuclear reassembly after mitosis |
title | Dephosphorylation in nuclear reassembly after mitosis |
title_full | Dephosphorylation in nuclear reassembly after mitosis |
title_fullStr | Dephosphorylation in nuclear reassembly after mitosis |
title_full_unstemmed | Dephosphorylation in nuclear reassembly after mitosis |
title_short | Dephosphorylation in nuclear reassembly after mitosis |
title_sort | dephosphorylation in nuclear reassembly after mitosis |
topic | Cell and Developmental Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9576876/ https://www.ncbi.nlm.nih.gov/pubmed/36268509 http://dx.doi.org/10.3389/fcell.2022.1012768 |
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