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Ileal and cecal microbiota response to Salmonella Typhimurium challenge in conventional and slow-growing broilers

Despite the negative impacts of Salmonella intestinal colonization on human health, Salmonella is a natural colonizer of the gastrointestinal tract and is not overtly pathogenic to the avian host. It is of interest to understand the impacts and colonization rates of Salmonella across selected geneti...

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Autores principales: Sheets, Tessa R., Wickware, Carmen L., Snyder, Ashlyn M., Weimer, Shawna L., Johnson, Timothy A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9577007/
https://www.ncbi.nlm.nih.gov/pubmed/36267582
http://dx.doi.org/10.3389/fphys.2022.971255
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author Sheets, Tessa R.
Wickware, Carmen L.
Snyder, Ashlyn M.
Weimer, Shawna L.
Johnson, Timothy A.
author_facet Sheets, Tessa R.
Wickware, Carmen L.
Snyder, Ashlyn M.
Weimer, Shawna L.
Johnson, Timothy A.
author_sort Sheets, Tessa R.
collection PubMed
description Despite the negative impacts of Salmonella intestinal colonization on human health, Salmonella is a natural colonizer of the gastrointestinal tract and is not overtly pathogenic to the avian host. It is of interest to understand the impacts and colonization rates of Salmonella across selected genetic lines such as slow-growing (SG) and conventional (CONV) broilers. The objective of this study was to characterize the relationship between Salmonella enterica serovar Typhimurium challenge and selected broiler genetic lines on the ileal and cecal microbiome. Male chicks of two broiler breeds (n = 156/breed) were cohoused in an open floor pen until day 7. On day 13, the chicks were then separated into 12 isolators per breed (4 rooms, 6 isolators/room, 11 chicks/isolator). On day 14, chicks in the 12 treatment isolators (6 isolators/breed, 108 total) were challenged with Salmonella Typhimurium (ST) (1 × [Formula: see text] CFU/ml) via oral gavage while the remaining chicks (n = 108) were given an oral gavage of sterile tryptic soy broth control (C). Ileal and cecal contents were collected on day 7 from 24 chicks of each breed, and on days 13, 17, 21, and 24 from two chicks per isolator. Samples underwent DNA extraction and PCR amplification to obtain 16S rRNA amplicons that were sequenced with Illumina MiSeq. Salmonella Typhimurium colonization in the cecum was not different in the two broiler breeds. The main effect of breed had the greatest impact on the ileum microbiota of broilers 7 days of age where SG broilers had significantly lower diversity and richness compared to CONV broilers (p < 0.05). Salmonella Typhimurium challenge consistently caused a change in beta diversity. Regardless of day or intestinal location, challenged broilers had many amplicon sequence variants (ASVs) with decreased abundance of likely beneficial bacteria such as Mollicutes RF39, Shuttleworthia, Flavonifractor, and Oscillibacter compared to broilers that were unchallenged with Salmonella Typhimurium (p < 0.05). Additionally, there was a difference in the timing of when the microbiota alpha and beta diversity of each breed responded to Salmonella Typhimurium challenge. Thus, both broiler breed and Salmonella Typhimurium can impact the intestinal microbiota.
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spelling pubmed-95770072022-10-19 Ileal and cecal microbiota response to Salmonella Typhimurium challenge in conventional and slow-growing broilers Sheets, Tessa R. Wickware, Carmen L. Snyder, Ashlyn M. Weimer, Shawna L. Johnson, Timothy A. Front Physiol Physiology Despite the negative impacts of Salmonella intestinal colonization on human health, Salmonella is a natural colonizer of the gastrointestinal tract and is not overtly pathogenic to the avian host. It is of interest to understand the impacts and colonization rates of Salmonella across selected genetic lines such as slow-growing (SG) and conventional (CONV) broilers. The objective of this study was to characterize the relationship between Salmonella enterica serovar Typhimurium challenge and selected broiler genetic lines on the ileal and cecal microbiome. Male chicks of two broiler breeds (n = 156/breed) were cohoused in an open floor pen until day 7. On day 13, the chicks were then separated into 12 isolators per breed (4 rooms, 6 isolators/room, 11 chicks/isolator). On day 14, chicks in the 12 treatment isolators (6 isolators/breed, 108 total) were challenged with Salmonella Typhimurium (ST) (1 × [Formula: see text] CFU/ml) via oral gavage while the remaining chicks (n = 108) were given an oral gavage of sterile tryptic soy broth control (C). Ileal and cecal contents were collected on day 7 from 24 chicks of each breed, and on days 13, 17, 21, and 24 from two chicks per isolator. Samples underwent DNA extraction and PCR amplification to obtain 16S rRNA amplicons that were sequenced with Illumina MiSeq. Salmonella Typhimurium colonization in the cecum was not different in the two broiler breeds. The main effect of breed had the greatest impact on the ileum microbiota of broilers 7 days of age where SG broilers had significantly lower diversity and richness compared to CONV broilers (p < 0.05). Salmonella Typhimurium challenge consistently caused a change in beta diversity. Regardless of day or intestinal location, challenged broilers had many amplicon sequence variants (ASVs) with decreased abundance of likely beneficial bacteria such as Mollicutes RF39, Shuttleworthia, Flavonifractor, and Oscillibacter compared to broilers that were unchallenged with Salmonella Typhimurium (p < 0.05). Additionally, there was a difference in the timing of when the microbiota alpha and beta diversity of each breed responded to Salmonella Typhimurium challenge. Thus, both broiler breed and Salmonella Typhimurium can impact the intestinal microbiota. Frontiers Media S.A. 2022-10-04 /pmc/articles/PMC9577007/ /pubmed/36267582 http://dx.doi.org/10.3389/fphys.2022.971255 Text en Copyright © 2022 Sheets, Wickware, Snyder, Weimer and Johnson. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Sheets, Tessa R.
Wickware, Carmen L.
Snyder, Ashlyn M.
Weimer, Shawna L.
Johnson, Timothy A.
Ileal and cecal microbiota response to Salmonella Typhimurium challenge in conventional and slow-growing broilers
title Ileal and cecal microbiota response to Salmonella Typhimurium challenge in conventional and slow-growing broilers
title_full Ileal and cecal microbiota response to Salmonella Typhimurium challenge in conventional and slow-growing broilers
title_fullStr Ileal and cecal microbiota response to Salmonella Typhimurium challenge in conventional and slow-growing broilers
title_full_unstemmed Ileal and cecal microbiota response to Salmonella Typhimurium challenge in conventional and slow-growing broilers
title_short Ileal and cecal microbiota response to Salmonella Typhimurium challenge in conventional and slow-growing broilers
title_sort ileal and cecal microbiota response to salmonella typhimurium challenge in conventional and slow-growing broilers
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9577007/
https://www.ncbi.nlm.nih.gov/pubmed/36267582
http://dx.doi.org/10.3389/fphys.2022.971255
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