Cargando…

Evaluation of graphene oxide, chitosan and their complex as antibacterial agents and anticancer apoptotic effect on HeLa cell line

Cancer and bacterial infection are the most serious problems threatening people's lives worldwide. However, the overuse of antibiotics as antibacterial and anticancer treatments can cause side effects and lead to drug-resistant bacteria. Therefore, developing natural materials with excellent an...

Descripción completa

Detalles Bibliográficos
Autores principales: Ashry, Noha M., El Bahgy, Halla E. K., Mohamed, Abdelkader, Alsubhi, Nouf H., Alrefaei, Ghadeer I., Binothman, Najat, Alharbi, Mona, Selim, Samy, Almuhayawi, Mohammed S., Alharbi, Mohanned T., Nagshabandi, Mohammed K., Saad, Ahmed M., El-Saadony, Mohamed T., Sitohy, Basel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9577200/
https://www.ncbi.nlm.nih.gov/pubmed/36267179
http://dx.doi.org/10.3389/fmicb.2022.922324
_version_ 1784811705789841408
author Ashry, Noha M.
El Bahgy, Halla E. K.
Mohamed, Abdelkader
Alsubhi, Nouf H.
Alrefaei, Ghadeer I.
Binothman, Najat
Alharbi, Mona
Selim, Samy
Almuhayawi, Mohammed S.
Alharbi, Mohanned T.
Nagshabandi, Mohammed K.
Saad, Ahmed M.
El-Saadony, Mohamed T.
Sitohy, Basel
author_facet Ashry, Noha M.
El Bahgy, Halla E. K.
Mohamed, Abdelkader
Alsubhi, Nouf H.
Alrefaei, Ghadeer I.
Binothman, Najat
Alharbi, Mona
Selim, Samy
Almuhayawi, Mohammed S.
Alharbi, Mohanned T.
Nagshabandi, Mohammed K.
Saad, Ahmed M.
El-Saadony, Mohamed T.
Sitohy, Basel
author_sort Ashry, Noha M.
collection PubMed
description Cancer and bacterial infection are the most serious problems threatening people's lives worldwide. However, the overuse of antibiotics as antibacterial and anticancer treatments can cause side effects and lead to drug-resistant bacteria. Therefore, developing natural materials with excellent antibacterial and anticancer activity is of great importance. In this study, different concentrations of chitosan (CS), graphene oxide (GO), and graphene oxide-chitosan composite (GO-CS) were tested to inhibit the bacterial growth of gram-positive (Bacillus cereus MG257494.1) and gram-negative (Pseudomonas aeruginosa PAO1). Moreover, we used the most efficient natural antibacterial material as an anticancer treatment. The zeta potential is a vital factor for antibacterial and anticancer mechanism, at pH 3–7, the zeta potential of chitosan was positive while at pH 7–12 were negative, however, the zeta potential for GO was negative at all pH values, which (p < 0.05) increased in the GO-CS composite. Chitosan concentrations (0.2 and 1.5%) exhibited antibacterial activity against BC with inhibition zone diameters of 4 and 12 mm, respectively, and against PAO1 with 2 and 10 mm, respectively. Treating BC and PAO1 with GO:CS (1:2) and GO:CS (1:1) gave a larger (p < 0.05) inhibition zone diameter. The viability and proliferation of HeLa cells treated with chitosan were significantly decreased (p < 0.05) from 95.3% at 0% to 12.93%, 10.33%, and 5.93% at 0.2%, 0.4%, and 0.60% concentrations of chitosan, respectively. Furthermore, CS treatment increased the activity of the P53 protein, which serves as a tumor suppressor. This study suggests that chitosan is effective as an antibacterial and may be useful for cancer treatment.
format Online
Article
Text
id pubmed-9577200
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-95772002022-10-19 Evaluation of graphene oxide, chitosan and their complex as antibacterial agents and anticancer apoptotic effect on HeLa cell line Ashry, Noha M. El Bahgy, Halla E. K. Mohamed, Abdelkader Alsubhi, Nouf H. Alrefaei, Ghadeer I. Binothman, Najat Alharbi, Mona Selim, Samy Almuhayawi, Mohammed S. Alharbi, Mohanned T. Nagshabandi, Mohammed K. Saad, Ahmed M. El-Saadony, Mohamed T. Sitohy, Basel Front Microbiol Microbiology Cancer and bacterial infection are the most serious problems threatening people's lives worldwide. However, the overuse of antibiotics as antibacterial and anticancer treatments can cause side effects and lead to drug-resistant bacteria. Therefore, developing natural materials with excellent antibacterial and anticancer activity is of great importance. In this study, different concentrations of chitosan (CS), graphene oxide (GO), and graphene oxide-chitosan composite (GO-CS) were tested to inhibit the bacterial growth of gram-positive (Bacillus cereus MG257494.1) and gram-negative (Pseudomonas aeruginosa PAO1). Moreover, we used the most efficient natural antibacterial material as an anticancer treatment. The zeta potential is a vital factor for antibacterial and anticancer mechanism, at pH 3–7, the zeta potential of chitosan was positive while at pH 7–12 were negative, however, the zeta potential for GO was negative at all pH values, which (p < 0.05) increased in the GO-CS composite. Chitosan concentrations (0.2 and 1.5%) exhibited antibacterial activity against BC with inhibition zone diameters of 4 and 12 mm, respectively, and against PAO1 with 2 and 10 mm, respectively. Treating BC and PAO1 with GO:CS (1:2) and GO:CS (1:1) gave a larger (p < 0.05) inhibition zone diameter. The viability and proliferation of HeLa cells treated with chitosan were significantly decreased (p < 0.05) from 95.3% at 0% to 12.93%, 10.33%, and 5.93% at 0.2%, 0.4%, and 0.60% concentrations of chitosan, respectively. Furthermore, CS treatment increased the activity of the P53 protein, which serves as a tumor suppressor. This study suggests that chitosan is effective as an antibacterial and may be useful for cancer treatment. Frontiers Media S.A. 2022-10-04 /pmc/articles/PMC9577200/ /pubmed/36267179 http://dx.doi.org/10.3389/fmicb.2022.922324 Text en Copyright © 2022 Ashry, El Bahgy, Mohamed, Alsubhi, Alrefaei, Binothman, Alharbi, Selim, Almuhayawi, Alharbi, Nagshabandi, Saad, El-Saadony and Sitohy. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Ashry, Noha M.
El Bahgy, Halla E. K.
Mohamed, Abdelkader
Alsubhi, Nouf H.
Alrefaei, Ghadeer I.
Binothman, Najat
Alharbi, Mona
Selim, Samy
Almuhayawi, Mohammed S.
Alharbi, Mohanned T.
Nagshabandi, Mohammed K.
Saad, Ahmed M.
El-Saadony, Mohamed T.
Sitohy, Basel
Evaluation of graphene oxide, chitosan and their complex as antibacterial agents and anticancer apoptotic effect on HeLa cell line
title Evaluation of graphene oxide, chitosan and their complex as antibacterial agents and anticancer apoptotic effect on HeLa cell line
title_full Evaluation of graphene oxide, chitosan and their complex as antibacterial agents and anticancer apoptotic effect on HeLa cell line
title_fullStr Evaluation of graphene oxide, chitosan and their complex as antibacterial agents and anticancer apoptotic effect on HeLa cell line
title_full_unstemmed Evaluation of graphene oxide, chitosan and their complex as antibacterial agents and anticancer apoptotic effect on HeLa cell line
title_short Evaluation of graphene oxide, chitosan and their complex as antibacterial agents and anticancer apoptotic effect on HeLa cell line
title_sort evaluation of graphene oxide, chitosan and their complex as antibacterial agents and anticancer apoptotic effect on hela cell line
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9577200/
https://www.ncbi.nlm.nih.gov/pubmed/36267179
http://dx.doi.org/10.3389/fmicb.2022.922324
work_keys_str_mv AT ashrynoham evaluationofgrapheneoxidechitosanandtheircomplexasantibacterialagentsandanticancerapoptoticeffectonhelacellline
AT elbahgyhallaek evaluationofgrapheneoxidechitosanandtheircomplexasantibacterialagentsandanticancerapoptoticeffectonhelacellline
AT mohamedabdelkader evaluationofgrapheneoxidechitosanandtheircomplexasantibacterialagentsandanticancerapoptoticeffectonhelacellline
AT alsubhinoufh evaluationofgrapheneoxidechitosanandtheircomplexasantibacterialagentsandanticancerapoptoticeffectonhelacellline
AT alrefaeighadeeri evaluationofgrapheneoxidechitosanandtheircomplexasantibacterialagentsandanticancerapoptoticeffectonhelacellline
AT binothmannajat evaluationofgrapheneoxidechitosanandtheircomplexasantibacterialagentsandanticancerapoptoticeffectonhelacellline
AT alharbimona evaluationofgrapheneoxidechitosanandtheircomplexasantibacterialagentsandanticancerapoptoticeffectonhelacellline
AT selimsamy evaluationofgrapheneoxidechitosanandtheircomplexasantibacterialagentsandanticancerapoptoticeffectonhelacellline
AT almuhayawimohammeds evaluationofgrapheneoxidechitosanandtheircomplexasantibacterialagentsandanticancerapoptoticeffectonhelacellline
AT alharbimohannedt evaluationofgrapheneoxidechitosanandtheircomplexasantibacterialagentsandanticancerapoptoticeffectonhelacellline
AT nagshabandimohammedk evaluationofgrapheneoxidechitosanandtheircomplexasantibacterialagentsandanticancerapoptoticeffectonhelacellline
AT saadahmedm evaluationofgrapheneoxidechitosanandtheircomplexasantibacterialagentsandanticancerapoptoticeffectonhelacellline
AT elsaadonymohamedt evaluationofgrapheneoxidechitosanandtheircomplexasantibacterialagentsandanticancerapoptoticeffectonhelacellline
AT sitohybasel evaluationofgrapheneoxidechitosanandtheircomplexasantibacterialagentsandanticancerapoptoticeffectonhelacellline