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Exploring the mechanism of immediate analgesic effect of 1-time tuina intervention in minor chronic constriction injury rats using RNA-seq

Previous studies have proved and investigated the mechanism of the analgesic effect of tuina treatment on neuropathic pain. The purpose of this study was to analyze changes in gene expression in the dorsal root ganglia (DRG) and spinal dorsal horn (SDH) after 1-time tuina intervention to investigate...

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Autores principales: Wang, Hourong, Liu, Zhifeng, Yu, Tianyuan, Zhang, Yingqi, Xu, Yajing, Jiao, Yi, Guan, Qian, Liu, Di
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9577287/
https://www.ncbi.nlm.nih.gov/pubmed/36267229
http://dx.doi.org/10.3389/fnins.2022.1007432
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author Wang, Hourong
Liu, Zhifeng
Yu, Tianyuan
Zhang, Yingqi
Xu, Yajing
Jiao, Yi
Guan, Qian
Liu, Di
author_facet Wang, Hourong
Liu, Zhifeng
Yu, Tianyuan
Zhang, Yingqi
Xu, Yajing
Jiao, Yi
Guan, Qian
Liu, Di
author_sort Wang, Hourong
collection PubMed
description Previous studies have proved and investigated the mechanism of the analgesic effect of tuina treatment on neuropathic pain. The purpose of this study was to analyze changes in gene expression in the dorsal root ganglia (DRG) and spinal dorsal horn (SDH) after 1-time tuina intervention to investigate the immediate analgesic mechanism by tuina. An improvement in nociceptive behavior in minor chronic constriction injury (CCI) rats after 1-time tuina was observed. 1-time tuina was more effective in the amelioration of thermal hyperalgesia, but no changes were found in the ultrastructure of DRG and SDH. Sixty-five differentially expressed genes (DEGs) modulated by tuina were detected in the DRG and 123 DEGs were detected in the SDH. Potential immediate analgesic mechanisms of tuina were analyzed by the Kyoto Encyclopedia of Genes and Genomes. DEGs were enriched in 75 pathways in DRG, and 107 pathways in SDH. The immediate analgesic mechanism of tuina is related to the calcium signaling pathway, thermogenesis, and regulation of lipolysis in adipocytes.
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spelling pubmed-95772872022-10-19 Exploring the mechanism of immediate analgesic effect of 1-time tuina intervention in minor chronic constriction injury rats using RNA-seq Wang, Hourong Liu, Zhifeng Yu, Tianyuan Zhang, Yingqi Xu, Yajing Jiao, Yi Guan, Qian Liu, Di Front Neurosci Neuroscience Previous studies have proved and investigated the mechanism of the analgesic effect of tuina treatment on neuropathic pain. The purpose of this study was to analyze changes in gene expression in the dorsal root ganglia (DRG) and spinal dorsal horn (SDH) after 1-time tuina intervention to investigate the immediate analgesic mechanism by tuina. An improvement in nociceptive behavior in minor chronic constriction injury (CCI) rats after 1-time tuina was observed. 1-time tuina was more effective in the amelioration of thermal hyperalgesia, but no changes were found in the ultrastructure of DRG and SDH. Sixty-five differentially expressed genes (DEGs) modulated by tuina were detected in the DRG and 123 DEGs were detected in the SDH. Potential immediate analgesic mechanisms of tuina were analyzed by the Kyoto Encyclopedia of Genes and Genomes. DEGs were enriched in 75 pathways in DRG, and 107 pathways in SDH. The immediate analgesic mechanism of tuina is related to the calcium signaling pathway, thermogenesis, and regulation of lipolysis in adipocytes. Frontiers Media S.A. 2022-10-04 /pmc/articles/PMC9577287/ /pubmed/36267229 http://dx.doi.org/10.3389/fnins.2022.1007432 Text en Copyright © 2022 Wang, Liu, Yu, Zhang, Xu, Jiao, Guan and Liu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Wang, Hourong
Liu, Zhifeng
Yu, Tianyuan
Zhang, Yingqi
Xu, Yajing
Jiao, Yi
Guan, Qian
Liu, Di
Exploring the mechanism of immediate analgesic effect of 1-time tuina intervention in minor chronic constriction injury rats using RNA-seq
title Exploring the mechanism of immediate analgesic effect of 1-time tuina intervention in minor chronic constriction injury rats using RNA-seq
title_full Exploring the mechanism of immediate analgesic effect of 1-time tuina intervention in minor chronic constriction injury rats using RNA-seq
title_fullStr Exploring the mechanism of immediate analgesic effect of 1-time tuina intervention in minor chronic constriction injury rats using RNA-seq
title_full_unstemmed Exploring the mechanism of immediate analgesic effect of 1-time tuina intervention in minor chronic constriction injury rats using RNA-seq
title_short Exploring the mechanism of immediate analgesic effect of 1-time tuina intervention in minor chronic constriction injury rats using RNA-seq
title_sort exploring the mechanism of immediate analgesic effect of 1-time tuina intervention in minor chronic constriction injury rats using rna-seq
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9577287/
https://www.ncbi.nlm.nih.gov/pubmed/36267229
http://dx.doi.org/10.3389/fnins.2022.1007432
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